中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2010年
11期
777-779
,共3页
钟珊%汪科%赵俊郁%李妍%陈喜雪%闫言%陈金波%徐浩翔%曾跃平%晋红中%朱学骏
鐘珊%汪科%趙俊鬱%李妍%陳喜雪%閆言%陳金波%徐浩翔%曾躍平%晉紅中%硃學駿
종산%왕과%조준욱%리연%진희설%염언%진금파%서호상%증약평%진홍중%주학준
天疱疮%桥粒芯糖蛋白1%酶联免疫吸附测定%荧光抗体技术,间接
天皰瘡%橋粒芯糖蛋白1%酶聯免疫吸附測定%熒光抗體技術,間接
천포창%교립심당단백1%매련면역흡부측정%형광항체기술,간접
Pemphigus%Desmoglein 1%Enzyme-linked immunosorbent assay%Fluorescent antibody technique,indirect
目的 探讨桥粒芯蛋白1(Dsg1)酶联免疫吸附试验(ELISA)检测落叶型天疱疮(PF)血清学抗体的意义.方法 将80例PF患者和132例对照人群的血清标本进行编盲,用ELISA法检测所有标本中抗Dsg1自身抗体,同时应用间接免疫荧光(IIF)法检测所有标本的抗体滴度,操作过程为随机检测,最后将两方法的结果进行比较.结果 75例PF患者和5例对照人群(包括1例不确定值,大疱性类天疱疮、SLE、皮肌炎、湿疹及健康者各1例)Dsg1 ELISA为阳性,71例PF患者和0例对照人群IIF为阳性.Dsg1ELISA的敏感性为93.8%(95%可信区间0.85～0.98),特异性为96.2%(95%可信区间0.91～0.99).IIF的敏感性为88.8%(95%可信区间0.82～0.96),特异性为100%(95%可信区间0.96～1.00).两者相比,敏感性(P=0.289)和特异性(P=1.000)差异均无统计学意义.结论 Dsg1 ELISA是一种简便、敏感、特异的血清学检测方法,可作为诊断PF的一种辅助手段.
目的 探討橋粒芯蛋白1(Dsg1)酶聯免疫吸附試驗(ELISA)檢測落葉型天皰瘡(PF)血清學抗體的意義.方法 將80例PF患者和132例對照人群的血清標本進行編盲,用ELISA法檢測所有標本中抗Dsg1自身抗體,同時應用間接免疫熒光(IIF)法檢測所有標本的抗體滴度,操作過程為隨機檢測,最後將兩方法的結果進行比較.結果 75例PF患者和5例對照人群(包括1例不確定值,大皰性類天皰瘡、SLE、皮肌炎、濕疹及健康者各1例)Dsg1 ELISA為暘性,71例PF患者和0例對照人群IIF為暘性.Dsg1ELISA的敏感性為93.8%(95%可信區間0.85～0.98),特異性為96.2%(95%可信區間0.91～0.99).IIF的敏感性為88.8%(95%可信區間0.82～0.96),特異性為100%(95%可信區間0.96～1.00).兩者相比,敏感性(P=0.289)和特異性(P=1.000)差異均無統計學意義.結論 Dsg1 ELISA是一種簡便、敏感、特異的血清學檢測方法,可作為診斷PF的一種輔助手段.
목적 탐토교립심단백1(Dsg1)매련면역흡부시험(ELISA)검측락협형천포창(PF)혈청학항체적의의.방법 장80례PF환자화132례대조인군적혈청표본진행편맹,용ELISA법검측소유표본중항Dsg1자신항체,동시응용간접면역형광(IIF)법검측소유표본적항체적도,조작과정위수궤검측,최후장량방법적결과진행비교.결과 75례PF환자화5례대조인군(포괄1례불학정치,대포성류천포창、SLE、피기염、습진급건강자각1례)Dsg1 ELISA위양성,71례PF환자화0례대조인군IIF위양성.Dsg1ELISA적민감성위93.8%(95%가신구간0.85～0.98),특이성위96.2%(95%가신구간0.91～0.99).IIF적민감성위88.8%(95%가신구간0.82～0.96),특이성위100%(95%가신구간0.96～1.00).량자상비,민감성(P=0.289)화특이성(P=1.000)차이균무통계학의의.결론 Dsg1 ELISA시일충간편、민감、특이적혈청학검측방법,가작위진단PF적일충보조수단.
Objective To evaluate the performance of desmoglein (Dsg)1 enzyme-linked immunosorbent assay (ELISA) in the detection of serum antibodies in patients with pemphigus foliaceus (PF). Methods Sera were obtained from 80 patients with PF and 132 human controls including 33 patients with bullous pemphigoid, 3 patients with linear IgA bullous dermatosis, 2 patients with acquired bullous epidermolysis, 20 patients with systemic lupus erythematosus (SLE), etc, and subjected to a random and blind test by Dsg1 ELISA and indirect immunofluorescence (IIF) on monkey oesophagus. Results The Dsg1 ELISA was positive in 75 (93.8%) patients with PF and 5 (3.8%) human controls (including 1 case of bullous pemphigoid, 1 case of SLE, 1 case of dermatomyositis, 1 case of eczema and 1 normal human control with indeterminate value), and IIF was positive in 71 (88.8%) patients with PF, but in none of the controls. The sensitivity and specificity was 93.8% (95% CI: 0.85 - 0.98) and 96.2% (95% CI: 0.91 - 0.99) respectively for Dsg1 ELISA in the serodiagnosis of PF, 88.8% (95% CI: 0.82 - 0.96) and 100% (95% CI: 0.96 - 1.00) respectively for IIF. There was no statistical difference in the sensitivities (P= 0.289) or specificities (P= 1.000) between the two test methods.Conclusions Dsg1 ELISA is a simple, sensitive and specific serological detection method, and can serve as an adjunct in the diagnosis of PF.
