中华创伤骨科杂志
中華創傷骨科雜誌
중화창상골과잡지
CHINESE JOURNAL OF ORTHOPAEDIC TRAUMA
2008年
3期
260-265
,共6页
王新涛%闫景龙%杨显声%麻松%周长龙%奚春阳
王新濤%閆景龍%楊顯聲%痳鬆%週長龍%奚春暘
왕신도%염경룡%양현성%마송%주장룡%해춘양
骨生成%移植%骨细胞
骨生成%移植%骨細胞
골생성%이식%골세포
Osteocytes%Grafting%Osteocyte
目的 观察微小颗粒骨在移植修复骨缺损过程中的骨细胞存活情况和生物活性. 方法 建立大鼠桡骨骨缺损模型,近交系DA大鼠88只,其中雄性大鼠28只,作为供体;雌性大鼠60只,作为受体.将受体随机分为块状骨组(n=56)、微小颗粒骨组(n=56)和空白对照组(n=4),取雄性大鼠髂骨为供体骨,分别制成直径为2mm的骨块和直径为300~500 μm的微小颗粒骨,植入骨缺损,于术后1 d、4 d、1周、2周、4周、6周、10周取材,采用原位杂交的方法观察受体内Y染色体性别决定基因(Sry)的表达情况,应用免疫组化法观察各组骨形态发生蛋白-2(BMP~2)、转化生长因子-β1(TGF-β1)、碱性磷酸酶(ALP)和I型胶原的表达情况. 结果 块状骨组在移植早期Srv的表达逐渐减少,至1周消失,4周后再次出现,并且随时间延长表达逐渐增多;微小颗粒骨组各时间段均有Sry的表达,在同一时间点,微小颗粒骨组Sry的阳性细胞数多于块状骨组(P<0.05),两种骨移植物中参与修复骨缺损的细胞类型不同.微小颗粒骨内和周围组织中BMP-2、TGF-β1、ALP和Ⅰ型胶原的阳性细胞数在术后2周内多于块状骨组(P<0.05). 结论 微小颗粒骨与块状骨修复骨缺损时均有供体骨细胞参与,但微小颗粒骨内有更多的骨细胞存活.微小颗粒骨内存活的骨细胞具有生物学活性,合成并分泌骨生长因子和骨基质蛋白,可以加速骨缺损的修复.
目的 觀察微小顆粒骨在移植脩複骨缺損過程中的骨細胞存活情況和生物活性. 方法 建立大鼠橈骨骨缺損模型,近交繫DA大鼠88隻,其中雄性大鼠28隻,作為供體;雌性大鼠60隻,作為受體.將受體隨機分為塊狀骨組(n=56)、微小顆粒骨組(n=56)和空白對照組(n=4),取雄性大鼠髂骨為供體骨,分彆製成直徑為2mm的骨塊和直徑為300~500 μm的微小顆粒骨,植入骨缺損,于術後1 d、4 d、1週、2週、4週、6週、10週取材,採用原位雜交的方法觀察受體內Y染色體性彆決定基因(Sry)的錶達情況,應用免疫組化法觀察各組骨形態髮生蛋白-2(BMP~2)、轉化生長因子-β1(TGF-β1)、堿性燐痠酶(ALP)和I型膠原的錶達情況. 結果 塊狀骨組在移植早期Srv的錶達逐漸減少,至1週消失,4週後再次齣現,併且隨時間延長錶達逐漸增多;微小顆粒骨組各時間段均有Sry的錶達,在同一時間點,微小顆粒骨組Sry的暘性細胞數多于塊狀骨組(P<0.05),兩種骨移植物中參與脩複骨缺損的細胞類型不同.微小顆粒骨內和週圍組織中BMP-2、TGF-β1、ALP和Ⅰ型膠原的暘性細胞數在術後2週內多于塊狀骨組(P<0.05). 結論 微小顆粒骨與塊狀骨脩複骨缺損時均有供體骨細胞參與,但微小顆粒骨內有更多的骨細胞存活.微小顆粒骨內存活的骨細胞具有生物學活性,閤成併分泌骨生長因子和骨基質蛋白,可以加速骨缺損的脩複.
목적 관찰미소과립골재이식수복골결손과정중적골세포존활정황화생물활성. 방법 건립대서뇨골골결손모형,근교계DA대서88지,기중웅성대서28지,작위공체;자성대서60지,작위수체.장수체수궤분위괴상골조(n=56)、미소과립골조(n=56)화공백대조조(n=4),취웅성대서가골위공체골,분별제성직경위2mm적골괴화직경위300~500 μm적미소과립골,식입골결손,우술후1 d、4 d、1주、2주、4주、6주、10주취재,채용원위잡교적방법관찰수체내Y염색체성별결정기인(Sry)적표체정황,응용면역조화법관찰각조골형태발생단백-2(BMP~2)、전화생장인자-β1(TGF-β1)、감성린산매(ALP)화I형효원적표체정황. 결과 괴상골조재이식조기Srv적표체축점감소,지1주소실,4주후재차출현,병차수시간연장표체축점증다;미소과립골조각시간단균유Sry적표체,재동일시간점,미소과립골조Sry적양성세포수다우괴상골조(P<0.05),량충골이식물중삼여수복골결손적세포류형불동.미소과립골내화주위조직중BMP-2、TGF-β1、ALP화Ⅰ형효원적양성세포수재술후2주내다우괴상골조(P<0.05). 결론 미소과립골여괴상골수복골결손시균유공체골세포삼여,단미소과립골내유경다적골세포존활.미소과립골내존활적골세포구유생물학활성,합성병분비골생장인자화골기질단백,가이가속골결손적수복.
Objective To investigate the bioactivity of osteocytes in tiny morselized bone grafts for bone defect repair. Methods Models of radial defect were established on 60 female svngeneic inbred DA rats which were divided into 3 groups randomly:structural bone grafting group(n=56),tiny morselized bone grafting group(n=56)and control group(without bone grafting, n=4).The ilia of 28 male inbred DA rats were harvested as donors and made into structural bone grafts 2mm in diameter and tiny morselized bone grafts 300~500 μm in diameter to be transplanted into the radial defects of the female receptors.Samples were harvested on 1 d,4 d, 1 w,2 w,4 w,6 w and 10 w after transplantation.The presence and relative amounts of genes specific to the sex-determining region of the Y-chromosome(Sty)originating from the bone grafts were measured by in situ hybridization(ISH).The expressions of bone morphogenetic proteins-2(BMP-2),transforming growth factor-betal (TGF-β1),alkaline phosphatase (ALP)and Collagen I were semiquantmed by immunohistochemistry. Results In structural bone grafting group,the expression of Sry decreased early and disappeared at 1 w.But after 4 w,Sry appeared again and its expression increased with lapse of time.In tiny morselized bone grafting group,Sry was detected all the time.At each time point.its expression was more than in Group I(P<0.05).Donor bone cells were different during repair in both groups.Tiny morselized bone grafts were absorbed quickly,and the expressions of BMP-2 and TGF-β1 reached the peak faster than in structural bone grafting group.The positive cells of BMP-2,TGF-β1,ALP and Collagen I in tiny morselized bone grafting group were much more than in structural bone grafting group during the first 2 w(P<0.05).Conclusions Both bone grafts can provide donor cells to repair bone defects.Compared with bulk bone grafts,tiny morselized bone grafts contain more living bioactive osteocytes which can secrete bone growth factors and bone matrix proteins,so they may accelerate healing of bone defects.