中国医药
中國醫藥
중국의약
CHINA MEDICINE
2011年
4期
415-417
,共3页
糖尿病%银杏叶提取物%基质金属蛋白酶类
糖尿病%銀杏葉提取物%基質金屬蛋白酶類
당뇨병%은행협제취물%기질금속단백매류
Diabetes%Ginkgo biloba extract%Matrix metalloproteinases
目的 观察银杏叶提取物(GbE)对雄性SD糖尿病大鼠肾脏基质金属蛋白酶2(MMP-2)和Ⅳ型胶原表达的影响,从而探讨银杏叶对糖尿病肾病的保护机制.方法 24只链脲佐菌素诱导的SD大鼠完全随机分成糖尿病对照组(DM组)和GbE组,并以12只正常雄性SD大鼠作为正常对照组(NC组).分别于4、8周后,观察各组大鼠体重、肾重、随机血糖、尿蛋白排泄率等生化指标,并用免疫组化和逆转录聚合酶链反应方法 观察糖尿病大鼠肾脏MMP-2及Ⅳ型胶原的表达.结果 糖尿病大鼠的随机血糖、体重、肾脏/体重明显下降,尿白蛋白排泄率明显升高[DM组(31.59±4.59)mg/24 h比NC组(8.74±2.08)mg/24 h,P＜0.05],GbE组糖尿病大鼠尿白蛋白排泄率[(21.38±3.86)mg/24 h]较DM组有所下降(P＜0.05).8周时,与正常对照组相比,DM组大鼠肾小球MMP-2蛋白的表达水平明显下降[(0.49±0.07)mg/24 h比(0.15±0.04)mg/24 h,P＜0.05],而GbE组DM组大鼠肾小球MMP-2蛋白的表达水平[(0.48±0.07)mg/24 h]较DM组无明显下降(P＞0.05);DM组大鼠Ⅳ型胶原的表达水平较NC组明显升高[(3.48±0.54)mg/24h比(1.68±0.51)mg/24 h],而GbE组糖尿病大鼠较NC组无明显升高[(2.55±0.50)mg/24 h比(1.68±0.51)mg/24 h].结论 GbE可通过抑制糖尿病大鼠MMP-2过表达,进而对糖尿病肾病发挥保护作用.
目的 觀察銀杏葉提取物(GbE)對雄性SD糖尿病大鼠腎髒基質金屬蛋白酶2(MMP-2)和Ⅳ型膠原錶達的影響,從而探討銀杏葉對糖尿病腎病的保護機製.方法 24隻鏈脲佐菌素誘導的SD大鼠完全隨機分成糖尿病對照組(DM組)和GbE組,併以12隻正常雄性SD大鼠作為正常對照組(NC組).分彆于4、8週後,觀察各組大鼠體重、腎重、隨機血糖、尿蛋白排洩率等生化指標,併用免疫組化和逆轉錄聚閤酶鏈反應方法 觀察糖尿病大鼠腎髒MMP-2及Ⅳ型膠原的錶達.結果 糖尿病大鼠的隨機血糖、體重、腎髒/體重明顯下降,尿白蛋白排洩率明顯升高[DM組(31.59±4.59)mg/24 h比NC組(8.74±2.08)mg/24 h,P＜0.05],GbE組糖尿病大鼠尿白蛋白排洩率[(21.38±3.86)mg/24 h]較DM組有所下降(P＜0.05).8週時,與正常對照組相比,DM組大鼠腎小毬MMP-2蛋白的錶達水平明顯下降[(0.49±0.07)mg/24 h比(0.15±0.04)mg/24 h,P＜0.05],而GbE組DM組大鼠腎小毬MMP-2蛋白的錶達水平[(0.48±0.07)mg/24 h]較DM組無明顯下降(P＞0.05);DM組大鼠Ⅳ型膠原的錶達水平較NC組明顯升高[(3.48±0.54)mg/24h比(1.68±0.51)mg/24 h],而GbE組糖尿病大鼠較NC組無明顯升高[(2.55±0.50)mg/24 h比(1.68±0.51)mg/24 h].結論 GbE可通過抑製糖尿病大鼠MMP-2過錶達,進而對糖尿病腎病髮揮保護作用.
목적 관찰은행협제취물(GbE)대웅성SD당뇨병대서신장기질금속단백매2(MMP-2)화Ⅳ형효원표체적영향,종이탐토은행협대당뇨병신병적보호궤제.방법 24지련뇨좌균소유도적SD대서완전수궤분성당뇨병대조조(DM조)화GbE조,병이12지정상웅성SD대서작위정상대조조(NC조).분별우4、8주후,관찰각조대서체중、신중、수궤혈당、뇨단백배설솔등생화지표,병용면역조화화역전록취합매련반응방법 관찰당뇨병대서신장MMP-2급Ⅳ형효원적표체.결과 당뇨병대서적수궤혈당、체중、신장/체중명현하강,뇨백단백배설솔명현승고[DM조(31.59±4.59)mg/24 h비NC조(8.74±2.08)mg/24 h,P＜0.05],GbE조당뇨병대서뇨백단백배설솔[(21.38±3.86)mg/24 h]교DM조유소하강(P＜0.05).8주시,여정상대조조상비,DM조대서신소구MMP-2단백적표체수평명현하강[(0.49±0.07)mg/24 h비(0.15±0.04)mg/24 h,P＜0.05],이GbE조DM조대서신소구MMP-2단백적표체수평[(0.48±0.07)mg/24 h]교DM조무명현하강(P＞0.05);DM조대서Ⅳ형효원적표체수평교NC조명현승고[(3.48±0.54)mg/24h비(1.68±0.51)mg/24 h],이GbE조당뇨병대서교NC조무명현승고[(2.55±0.50)mg/24 h비(1.68±0.51)mg/24 h].결론 GbE가통과억제당뇨병대서MMP-2과표체,진이대당뇨병신병발휘보호작용.
Objective To study the effect of ginkgo biloba extract (GbE) on the expression of matrix metalloproteinase (MMP)-2 in kidneys of diabetic rates and to analyze the protecting mechanism of GbE on diabetic nephropathy. Methods Totally 24 SD rats of diabetic nephropathy induced by streptozotocin were randomly divided into two groups: diabetes group and treatment group with GbE, and 12 rats with placebo therapy were enrolled as the normal control group. Rats were killed after 4 ～ 8 weeks treatment and the expression of MMP-2 and type Ⅳ collagen were studies by immunohistochemistry and RT-PCR. Results In diabetic rats, blood sugar, weight and kidney/body weight ratio were decreased significantly and the excretion of 24 hour urinary protein was increased significantly compared to the diabetes group. But the excretion of 24 hour urinary protein was improved in GbE group ( P ＜0.05 ). The expression level of MMP-2 protein and mRNA was decreased significantly in renal glomduri of diabetic rats ( P ＜ 0.05 ) but showed no changes in GbE treatment group compared to diabetes group( P ＞ 0.05 ). The expression level of type Ⅳ collagen was significantly increased in diabetic rats( P ＜0.05 ) but showed no changes in GbE group( P ＞0.05). Conclusion GbE can improve diabetic nephropathy by inhibiting the expression of MMP-2 in diabetic Rat.
