中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2010年
7期
967-969
,共3页
王珏%黄宗强%王义生%刘宏建
王玨%黃宗彊%王義生%劉宏建
왕각%황종강%왕의생%류굉건
椎间盘%Sox9基因%基因治疗
椎間盤%Sox9基因%基因治療
추간반%Sox9기인%기인치료
Intervertral disc%Sox9 gene%Gene therapy
目的 观察腺病毒介导的Sox9基因(AdSox9)对兔腰椎间盘退变模型的疗效.方法 25只新西兰大白兔用针刺法制作腰椎间盘退变模型,24周后,将AdSox9注射到退变椎间盘组织中,6周后用MRI观察椎间盘变化,免疫组织化学法观察椎间盘组织Ⅱ型胶原(Col2al)的表达,逆转录-聚合酶链反应(RT-PCR)观察Col2al mRNA的变化.结果 MRI示:治疗后L2～3、L3～4、L4～5的椎间盘信号值分别为:580.43±76.43、612.74±56.73、605.87±76.53,模型对照组为:238.43±43.65、217.65±38.95、236.98±76.53;Col2al表达的标记指数:治疗后L2～3、L3～4、L4～5的椎间盘分别为:42.48±5.53、45.34±15.43、42.47±25.45,模型对照组为:11.68±10.08、12.68±13.75、13.38±26.53;Col2al mRNA的表达:治疗后L2～3、L3～4、L4～5的椎间盘分别为6570.43±174.53、7653.75±158.93、6905.83±119.23,模型对照组为:1256.76±89.76、1217.65±18.65、1132.97±47.65.3种方法均示,治疗组与模型对照组之间比较差异有统计学意义(P<0.01).结论 AdSox9对退变椎间盘动物模型有治疗作用.
目的 觀察腺病毒介導的Sox9基因(AdSox9)對兔腰椎間盤退變模型的療效.方法 25隻新西蘭大白兔用針刺法製作腰椎間盤退變模型,24週後,將AdSox9註射到退變椎間盤組織中,6週後用MRI觀察椎間盤變化,免疫組織化學法觀察椎間盤組織Ⅱ型膠原(Col2al)的錶達,逆轉錄-聚閤酶鏈反應(RT-PCR)觀察Col2al mRNA的變化.結果 MRI示:治療後L2～3、L3～4、L4～5的椎間盤信號值分彆為:580.43±76.43、612.74±56.73、605.87±76.53,模型對照組為:238.43±43.65、217.65±38.95、236.98±76.53;Col2al錶達的標記指數:治療後L2～3、L3～4、L4～5的椎間盤分彆為:42.48±5.53、45.34±15.43、42.47±25.45,模型對照組為:11.68±10.08、12.68±13.75、13.38±26.53;Col2al mRNA的錶達:治療後L2～3、L3～4、L4～5的椎間盤分彆為6570.43±174.53、7653.75±158.93、6905.83±119.23,模型對照組為:1256.76±89.76、1217.65±18.65、1132.97±47.65.3種方法均示,治療組與模型對照組之間比較差異有統計學意義(P<0.01).結論 AdSox9對退變椎間盤動物模型有治療作用.
목적 관찰선병독개도적Sox9기인(AdSox9)대토요추간반퇴변모형적료효.방법 25지신서란대백토용침자법제작요추간반퇴변모형,24주후,장AdSox9주사도퇴변추간반조직중,6주후용MRI관찰추간반변화,면역조직화학법관찰추간반조직Ⅱ형효원(Col2al)적표체,역전록-취합매련반응(RT-PCR)관찰Col2al mRNA적변화.결과 MRI시:치료후L2～3、L3～4、L4～5적추간반신호치분별위:580.43±76.43、612.74±56.73、605.87±76.53,모형대조조위:238.43±43.65、217.65±38.95、236.98±76.53;Col2al표체적표기지수:치료후L2～3、L3～4、L4～5적추간반분별위:42.48±5.53、45.34±15.43、42.47±25.45,모형대조조위:11.68±10.08、12.68±13.75、13.38±26.53;Col2al mRNA적표체:치료후L2～3、L3～4、L4～5적추간반분별위6570.43±174.53、7653.75±158.93、6905.83±119.23,모형대조조위:1256.76±89.76、1217.65±18.65、1132.97±47.65.3충방법균시,치료조여모형대조조지간비교차이유통계학의의(P<0.01).결론 AdSox9대퇴변추간반동물모형유치료작용.
Objective To assess adenoviral delivery vectors expressing Sox9 gene ( AdSox9) therapy for intervertral degenerative disc of an animal model. Methods The model of intervertebral degenerative disc in 25 rabbits was established by stabbing into the L2-3, L3-4 and L4-5 discs to produce a puncture injury and after 24 weeks, the intervertebral generative discs were injected with adSox9. After 30 weeks (6 weeks after injecting adSox9) the change of the intervertebral generative discs was observed by MRI, and the intervertebral generative discs were removed for detection of type 2 collagen ( Col2al) by immunohisto-chemistry and Col2al mRNA by reverse transcription-polymerase chain reaction ( RT-PCR) respectively. Results MRI showed after AdSox9 therapy the signal values of L2-3 , L3-4, L4-5 were 580.43±76.43, 612. 74 ± 56. 73, 605. 87 ± 76.53 respectively, and those in model control group were 238. 43 ± 43.65, 217. 65 ±38. 95, 236. 98 ±76. 53 respectively. The labeling index of Col2al expression in L2-3, L3-4, L4-5 after AdSox9 therapy was 42.48 ± 5.53, 45. 34 ± 15.43, 42.47 ± 25.45 respectively, and that in model control group was 11.68 ± 10.08, 12.68 ± 13.75, 13.38 ±26.53 respectively. The labeling index of Col2al mRNA expression in hyperhidrosis was 6570.43 ± 174. 53, 7653.75 ± 158. 93, 6905. 83 ±119.23 respectively, and that in the model control group was 1256. 76 ± 89. 76, 1217.65 ± 18. 65, 1132.97 ± 47. 65 respectively. All showed significant difference between the therapy group and the model control group (P< 0.01). Conclusion adSox9 therapy for intervertral degenerative disc of the animal model is effective.
