中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2008年
9期
1016-1020
,共5页
杨瑞锋%徐国宾%吴煦%李志艳%夏铁安
楊瑞鋒%徐國賓%吳煦%李誌豔%夏鐵安
양서봉%서국빈%오후%리지염%하철안
肾移植%巨细胞病毒%CD8阳性T淋巴细胞%T淋巴细胞,细胞毒性%干扰素Ⅱ型
腎移植%巨細胞病毒%CD8暘性T淋巴細胞%T淋巴細胞,細胞毒性%榦擾素Ⅱ型
신이식%거세포병독%CD8양성T림파세포%T림파세포,세포독성%간우소Ⅱ형
Kidney transplantation%Cytomegalovirus%CD8-positive T-lymphocytes%T-lymphocytes,cytotoxic%Interferon type Ⅱ
目的 研究肾移植患者外周血人巨细胞病毒(HCMV)特异性细胞毒T淋巴细胞(CTL)的频率及其γ干扰素(IFN-γ)分泌功能,以期探讨HCMV活化感染的免疫学机制.方法 选取HCMV血清学阳性的HLA-A*0201肾移植患者和健康对照者,将其分为3组:存在HCMV活化感染的肾移植组(9例)、无HCMV活化感染的肾移植组(29例)和健康对照组(54名);运用HLA-A2-NLVPMVATV-五聚体检测外周血中总的HCMV NLVPMVATV特异性CTL,同时以NLVPMVATV体外刺激PBMC,检测IFN-γ分泌性CTL.结果 在健康对照组、元HCMV活化感染的肾移植组和存在HCMV活化感染组中,HCMV特异性CTL频率中位数分别为1.19%(0～19.42%)、1.20%(0～18.40%)和3.2%(0.51%～18.90%),差异无统计学意义(H=5.34,P＞0.05);IFN-γ分泌细胞频率中位数分别为0.72%(0～0.70%),0.47%(0～5.61%)和0.67%(0.07%～4.00%),差异无统计学意义(H=0.58,P＞0.05),但HCMV特异性CTL中IFN-γ分泌细胞的比例分别为(60.18±19.16)%,(39.19±17.22)%和(20.02±13.26)%,组间比较差异有统计学意义(P＜0.01).结论 肾移植组外周血中HCMV NLVPMVATV特异性CTL频率较健康对照组并无降低,但其分泌IFN-γ的比例降低,出现HCMV活化感染组IFN-γ分泌CTL比例降低更为明显.HCMV特异性CTL的IFN-γ分泌功能受损可能是造成肾移植患者HCMV活化感染的因素之一.
目的 研究腎移植患者外週血人巨細胞病毒(HCMV)特異性細胞毒T淋巴細胞(CTL)的頻率及其γ榦擾素(IFN-γ)分泌功能,以期探討HCMV活化感染的免疫學機製.方法 選取HCMV血清學暘性的HLA-A*0201腎移植患者和健康對照者,將其分為3組:存在HCMV活化感染的腎移植組(9例)、無HCMV活化感染的腎移植組(29例)和健康對照組(54名);運用HLA-A2-NLVPMVATV-五聚體檢測外週血中總的HCMV NLVPMVATV特異性CTL,同時以NLVPMVATV體外刺激PBMC,檢測IFN-γ分泌性CTL.結果 在健康對照組、元HCMV活化感染的腎移植組和存在HCMV活化感染組中,HCMV特異性CTL頻率中位數分彆為1.19%(0～19.42%)、1.20%(0～18.40%)和3.2%(0.51%～18.90%),差異無統計學意義(H=5.34,P＞0.05);IFN-γ分泌細胞頻率中位數分彆為0.72%(0～0.70%),0.47%(0～5.61%)和0.67%(0.07%～4.00%),差異無統計學意義(H=0.58,P＞0.05),但HCMV特異性CTL中IFN-γ分泌細胞的比例分彆為(60.18±19.16)%,(39.19±17.22)%和(20.02±13.26)%,組間比較差異有統計學意義(P＜0.01).結論 腎移植組外週血中HCMV NLVPMVATV特異性CTL頻率較健康對照組併無降低,但其分泌IFN-γ的比例降低,齣現HCMV活化感染組IFN-γ分泌CTL比例降低更為明顯.HCMV特異性CTL的IFN-γ分泌功能受損可能是造成腎移植患者HCMV活化感染的因素之一.
목적 연구신이식환자외주혈인거세포병독(HCMV)특이성세포독T림파세포(CTL)적빈솔급기γ간우소(IFN-γ)분비공능,이기탐토HCMV활화감염적면역학궤제.방법 선취HCMV혈청학양성적HLA-A*0201신이식환자화건강대조자,장기분위3조:존재HCMV활화감염적신이식조(9례)、무HCMV활화감염적신이식조(29례)화건강대조조(54명);운용HLA-A2-NLVPMVATV-오취체검측외주혈중총적HCMV NLVPMVATV특이성CTL,동시이NLVPMVATV체외자격PBMC,검측IFN-γ분비성CTL.결과 재건강대조조、원HCMV활화감염적신이식조화존재HCMV활화감염조중,HCMV특이성CTL빈솔중위수분별위1.19%(0～19.42%)、1.20%(0～18.40%)화3.2%(0.51%～18.90%),차이무통계학의의(H=5.34,P＞0.05);IFN-γ분비세포빈솔중위수분별위0.72%(0～0.70%),0.47%(0～5.61%)화0.67%(0.07%～4.00%),차이무통계학의의(H=0.58,P＞0.05),단HCMV특이성CTL중IFN-γ분비세포적비례분별위(60.18±19.16)%,(39.19±17.22)%화(20.02±13.26)%,조간비교차이유통계학의의(P＜0.01).결론 신이식조외주혈중HCMV NLVPMVATV특이성CTL빈솔교건강대조조병무강저,단기분비IFN-γ적비례강저,출현HCMV활화감염조IFN-γ분비CTL비례강저경위명현.HCMV특이성CTL적IFN-γ분비공능수손가능시조성신이식환자HCMV활화감염적인소지일.
Objective To investigate the level of human cytomegalovirus(HCMV)specific CD8+ T lymphoeytes in peripheral blood and the immune reaponae of HCMV reactivation after kidney transplantation.Methods Thirty-eight HCMV seropesitive HLA-A*0201 kidney transplant recipients(9 with HCMV infection and 29 without HCMV infection)and 54 healthy individuals were enrolled.The levels of total HCMV specific CD8+ T cells were measured using HLA-A2 pentamer folded with HCMV-peptide NLVPMVATV.The levels of IFN-γ secreting CD8+ T cells were measured by intracelluhr IEN-γ staining pulsed with the same peptide.Results The median levels of pentamer stained CD8+ T cells were 1.19%(0-19.42%),1.20%(0-18.40%)and 3.2%(0.51%-18.90%)in healthy group,negative HCMV group and positive HCMV group(H=5.34,P＞0.05),respectively.The median levels of IFN-γ secreting CD8+ T cells were 0.72%(0-0.70%),0.47%(0-5.61%)and 0.67%(0.07%-4.00%),respectively(H=0.58,P＞0.05).However,the mean proportions of IFN-γ secreting pentamer stained T cells relative to total HCMV specifc CIL were(60.18±19.16)%,(39.19±17.22)% and(20.02±13.26)%,respectively.There were significant differences among the groups(P＜0.01).Condusiorm There was no significant difference of levels of HCMV specific CD8+ T lymphocytes in peripheral blood between the kidney transplant recipients and healthy individuals.However,the proportion of HCMV-specific IFN-γ producing CD8+ T cells in pentamer stained cells was reduced in the kidney transplant recipients especially in those with active HCMV infection,which may contribute to the inability to control HCMV reactivation.
