CAJ | 학술논문

为探明稻鸭共养模式抑制水稻纹枯病发生的原因, 以稻鸭共养模式的鸭粪提取物为研究对象, 对其抑菌活性及有效成分进行了测定和分析, 以期为水稻纹枯病生物防治提供新的途径.结果表明: 鸭粪乙醇提取物对水稻纹枯病菌有抑制作用, 24 h内最高菌丝生长抑制率和EC_(50)分别为88.64%、26.11 mg·mL~(-1); 鸭粪乙醇提取物经萃取分离得到石油醚和乙酸乙酯两种组分, 其中石油醚组分抑菌效果较弱, 乙酸乙酯组分抑菌能力较强, 其对水稻纹枯病菌抑制率为89.70%, EC_(50)为15.52 mg·mL~(-1); 对乙酸乙酯组分进行硅胶柱层析分离得到5种流份, 其中第2流份有显著抑菌活性, 在24 h和48 h内对水稻纹枯病菌的抑制率分别为89.58%和80.36%.GC-MS分析表明, 鸭粪的抑菌物质主要为酚酸类和含硫醇有机物质, 进一步纯化和鉴定有待研究.
위탐명도압공양모식억제수도문고병발생적원인, 이도압공양모식적압분제취물위연구대상, 대기억균활성급유효성분진행료측정화분석, 이기위수도문고병생물방치제공신적도경.결과표명: 압분을순제취물대수도문고병균유억제작용, 24 h내최고균사생장억제솔화EC_(50)분별위88.64%、26.11 mg·mL~(-1); 압분을순제취물경췌취분리득도석유미화을산을지량충조분, 기중석유미조분억균효과교약, 을산을지조분억균능력교강, 기대수도문고병균억제솔위89.70%, EC_(50)위15.52 mg·mL~(-1); 대을산을지조분진행규효주층석분리득도5충류빈, 기중제2류빈유현저억균활성, 재24 h화48 h내대수도문고병균적억제솔분별위89.58%화80.36%.GC-MS분석표명, 압분적억균물질주요위분산류화함류순유궤물질, 진일보순화화감정유대연구.
Duck manure from rice-duck eco-farming plays a crucial role in controlling Rhizoctonia solani. In this study, the anti-bacterial activity and effective components of duck manure extracts were analyzed for R. solani. Duck manure extracts via ethanol respectively has highest inhibition rate of 88.64% and EC_(50) of 26.11 mg·mL~(-1) against R. solani. The extract was further separated into two parts and one part separated by ethyl acetate while the other was separated by petroleum ether. Based on the inhibition ex-periment against R. solani, ethly acetate extract has a rather strong effect on R. solani with 89.70% inhibition rate and 15.52 mg·mL~(-1) EC_(50). The effect of petroleum ether extract on R. solani is relatively weak. The former was again divided into five parts in silica gel column, of which the second part significantly inhibits R. solani. During the periods of 24 and 48 hrs, the inhibition rate of the second part on R. solan is respectively 89.58% and 80.36%. GC-MS analysis suggests that the second part contains phenol sour and sulfur alcohol, which best suits further purification and identification.