CAJ | 학술논문

利用从蚕沙中提取的叶绿素(CHL)制得了锌叶绿素(Zn-CHL)配合物.采用紫外-可见光谱、CD光谱和黏度法研究了Zn-CHL与小牛胸腺DNA(ct-DNA)的相互作用,并采用琼脂糖凝胶电泳研究了光照条件下Zn-CHL对pBR322 DNA的切割能力.结果表明,Zn-CHL与ct-DNA的作用方式属于外部结合模式,其结合常数为2.32×10~5 L/mol,Zn-CHL在可见光照射下具有切割DNA的能力.
이용종잠사중제취적협록소(CHL)제득료자협록소(Zn-CHL)배합물.채용자외-가견광보、CD광보화점도법연구료Zn-CHL여소우흉선DNA(ct-DNA)적상호작용,병채용경지당응효전영연구료광조조건하Zn-CHL대pBR322 DNA적절할능력.결과표명,Zn-CHL여ct-DNA적작용방식속우외부결합모식,기결합상수위2.32×10~5 L/mol,Zn-CHL재가견광조사하구유절할DNA적능력.
Zinc chlorophyll(Zn-CHL) complex was prepared using chlorophyll extacted from silkworm faeces. Relative viscosity, UV-Visible and CD spectra were used to study the interaction between Zn-CHL and ct-DNA. Agarose gel electrophoresis was also carried out to investigate the photocleavage ability of Zn-CHL to pBR 322 DNA. The results suggested that the binding mode of Zn-CHL and ct-DNA was outside binding, the binding constant was 2 32×10~5 L/mol. Agarose gel electrophoresis picture showed that Zn-CHL could induce DNA cleavage upon irradiation.