CAJ | 학술논문

目的通过检测Wnt-1诱导分泌蛋白-1 (Wnt-1 induced secreted protein-1,WISP-1)在人乳腺癌中的表达,探讨其在乳腺癌发生发展中的作用.方法采用实时荧光定量RT-PCR和免疫组化法检测WISP-1 mRNA和蛋白表达水平, 定量分析其表达与临床病理特征的关系.结果 WISP-1在乳腺癌组织中的表达水平高于癌旁正常乳腺组织,差异有统计学意义(P=0.001).WISP-1 mRNA表达与肿瘤大小、分期、组织学类型、是否有淋巴结转移和表皮生长因子受体-2(HER-2)表达有关(P<0.05),与年龄和雌、孕激素受体无关(P>0.05).WISP-1蛋白表达与肿瘤组织学类型、是否有淋巴结转移和HER-2表达有关(P<0.05),与年龄,肿瘤大小,分期和雌、孕激素受体无关(P>0.05).结论乳腺癌组织中WISP-1表达明显升高, 在乳腺癌的浸润与转移中具有重要作用.
목적 통과검측Wnt-1유도분비단백-1 (Wnt-1 induced secreted protein-1,WISP-1)재인유선암중적표체,탐토기재유선암발생발전중적작용.방법 채용실시형광정량RT-PCR화면역조화법검측WISP-1 mRNA화단백표체수평, 정량분석기표체여림상병리특정적관계.결과 WISP-1재유선암조직중적표체수평고우암방정상유선조직,차이유통계학의의(P=0.001).WISP-1 mRNA표체여종류대소、분기、조직학류형、시부유림파결전이화표피생장인자수체-2(HER-2)표체유관(P<0.05),여년령화자、잉격소수체무관(P>0.05).WISP-1단백표체여종류조직학류형、시부유림파결전이화HER-2표체유관(P<0.05),여년령,종류대소,분기화자、잉격소수체무관(P>0.05).결론 유선암조직중WISP-1표체명현승고, 재유선암적침윤여전이중구유중요작용.
Objective To investigate the expression of Wnt-1 induced secreted protein-1(WISP-1) between breast cancer and paired normal breast tissues and to explore the significance of WISP-1 in breast cancer tumorigenesis. Methods The mRNA and protein expressions of WISP-1 in human breast cancer were measured by Quantitative Real-Time RT-PCR and immunohistochemical staining and further analyzed the relationship between WISP-1 expression and clinic pathologic characters. Results WISP-1 expression in breast cancer was higher than that in normal breast tissue (P=0.001). The mRNA expression level of WISP-1 was correlated with tumor size, staging, lymph node status, differentiated degree and HER-2 status (P<0.05). WISP-1 protein expression level was correlated with lymph node status, differentiated degree and HER-2 status (P<0.05). Conclusion WISP-1 expression in human breast cancer increases significantly and may play a key role in the invasion and metastasis of human breast cancer.