CAJ | 학술논문

为研究硝酸铈对盾叶薯蓣快速繁殖的影响,采用以盾叶薯蓣茎段为外植体,在其愈伤诱导培养基、丛生芽培养基及生根培养基中添加不同浓度的Ce~(3+).结果表明,含Ce~(3+)为5mg/L的培养基可显著提高愈伤组织诱导率, 含Ce~(3+)1mg/L的培养基丛生芽诱导率最高;1～15mg/L的Ce~(3+) 对盾叶薯蓣组培苗生根有明显的促进作用,5mg/L的Ce~(3+)显示出最强的促进效应.可以认为,一定浓度Ce~(3+)对诱导愈伤组织生长、丛生芽萌发及不定根生长有促进作用,但高浓度的Ce~(3+)均对其呈现抑制效应.
위연구초산시대순협서여쾌속번식적영향,채용이순협서여경단위외식체,재기유상유도배양기、총생아배양기급생근배양기중첨가불동농도적Ce~(3+).결과표명,함Ce~(3+)위5mg/L적배양기가현저제고유상조직유도솔, 함Ce~(3+)1mg/L적배양기총생아유도솔최고;1～15mg/L적Ce~(3+) 대순협서여조배묘생근유명현적촉진작용,5mg/L적Ce~(3+)현시출최강적촉진효응.가이인위,일정농도Ce~(3+)대유도유상조직생장、총생아맹발급불정근생장유촉진작용,단고농도적Ce~(3+)균대기정현억제효응.
To study the effect of rare earth Ce(NO_3)_3 on rapid clonal propagation of Dioscorea zingiberensis. Using stems of Dioscorea zingiberensis as explants, different concentrations of Ce (NO_3)_3 were supplemented to the medium (1/2MS+0. 5μmol/L IBA) to study the effect of Ce~(3+) on the callus induction medium, adventitious bud initial medium and adventitious root medium. The callus induction was stimulated by 5.0mg/L Ce~(3+); 1.0m g/L Ce~(3+) stimulated the bud initial induction; while 1～15mg/L stimulated adventitious root. The callus induction adventitious bud initial and rooting was stimulated by the low concentrations of rare earth elements, but higher concentration inhibited them.