CAJ | 학술논문

本研究从菌株内生定殖、促生、抑制和诱导防御酶活性4方面探讨了红树内生细菌RS261菌株防治辣椒疫病的机理.结果表明,该菌株能进入辣椒等多种陆生植物体内定殖,在辣椒体内的定殖时间达26 d以上,且对辣椒生长表现有明显的促进作用;抑制作用测定发现,RS261能够分泌产生抗菌物质抑制辣椒疫霉菌丝的生长和游动孢子囊的形成;辣椒体内丙二醛(MDA)含量及防御性酶活测定结果显示,经过RS261培养液处理后,辣椒苗体内丙二醛(MDA)含量和超氧化歧化酶(SOD)、过氧化物酶(POD)以及过氧化氢酶(CAT)的活性等均较仅接种疫霉病菌的处理低,但可显著诱导辣椒体内苯丙氨酸解氨酶(PAL)的活性.
본연구종균주내생정식、촉생、억제화유도방어매활성4방면탐토료홍수내생세균RS261균주방치랄초역병적궤리.결과표명,해균주능진입랄초등다충륙생식물체내정식,재랄초체내적정식시간체26 d이상,차대랄초생장표현유명현적촉진작용;억제작용측정발현,RS261능구분비산생항균물질억제랄초역매균사적생장화유동포자낭적형성;랄초체내병이철(MDA)함량급방어성매활측정결과현시,경과RS261배양액처리후,랄초묘체내병이철(MDA)함량화초양화기화매(SOD)、과양화물매(POD)이급과양화경매(CAT)적활성등균교부접충역매병균적처리저,단가현저유도랄초체내분병안산해안매(PAL)적활성.
The mechanism of biological control of Phytophthora blight by treatment with mangrove endophytic bacterium RS261 was studied.Tagged by the resistance to rifampicin(300 μg/ML),the strain RS261could colonize many plants,such as capsicum,tomato,eggplant,et al.An obvious promotion on pepper growth occurred after watering roots with strain RS261.The metabolites of strain RS261 showed strong inhibitory activity against mycelial growth and sporangia production of P.capsici.Inoculation with P.capsici considerably increased the content of MDA and the activities of SOD,CAT and POD in the capsicum.However,when co-inoculated with strain RS261 and P.capsici at the same time,the MDA content and SOD,POD and CAT activities were all lower than those inoculated with pathogen only.In addition.treatment by co-inoculation increased PAL activity.and the induced PAL activity might enhance ant-disease capability of the plants.