CAJ | 학술논문

目的研究慢性乙型肝炎患者不同病毒载量与乙肝三系统及前S1抗原(Pre-S1Ag)之间的相关性分析.方法血清HBV DNA检测采用荧光定量PCR法(FQ-PCR),乙肝三系统及前S1抗原(Pre-S1Ag)检测采用ELISA法;对87例慢性乙型肝炎患者血清进行乙型肝炎病毒载量检测,以乙型肝炎病毒载量为标准,分为A组:(103～104)拷贝/mL,B组:(105～106)拷贝/mL,C组:>107拷贝/mL,检测乙肝三系统及Pre-S1Ag.结果 A组:(103～104)拷贝/mL,检出率最高的模式为小三阳、Pre-S1Ag(一).B组:(105～106)拷贝/mL,检出率最高的模式为小三阳、PreS1Ag(+);C组:>107拷贝/mL,检出率最高的模式为大三阳、Pre-S1Ag(+).结论乙肝三系统、前S1抗原与HBV DNA定量水平之间具有良好的相关性,能敏感地反映乙型肝炎病毒复制情况.
목적 연구만성을형간염환자불동병독재량여을간삼계통급전S1항원(Pre-S1Ag)지간적상관성분석.방법 혈청HBV DNA검측채용형광정량PCR법(FQ-PCR),을간삼계통급전S1항원(Pre-S1Ag)검측채용ELISA법;대87례만성을형간염환자혈청진행을형간염병독재량검측,이을형간염병독재량위표준,분위A조:(103～104)고패/mL,B조:(105～106)고패/mL,C조:>107고패/mL,검측을간삼계통급Pre-S1Ag.결과 A조:(103～104)고패/mL,검출솔최고적모식위소삼양、Pre-S1Ag(일).B조:(105～106)고패/mL,검출솔최고적모식위소삼양、PreS1Ag(+);C조:>107고패/mL,검출솔최고적모식위대삼양、Pre-S1Ag(+).결론 을간삼계통、전S1항원여HBV DNA정량수평지간구유량호적상관성,능민감지반영을형간염병독복제정황.
Objective To study the correlation of virus load with Three system of Hepatitis B and Pre-S1 antigen in patients with chronic hepatitis B. Methods The virus load of serum were detected by using FQ-PCR in 87 patients with chronic hepatitis B, while Three system of hepatitis B and Pre-S1 antigen were detected with ELISA. These results were analysed in four groups accod-ing to virus load. Results A group with 103～104 copies/mL,the common model is HBsAg( + ) , HBeAb( + ) ,HBcAb( + ) andPreSK-);B group with 105～106 copies/mL, the common model is HBsAg( + ) , HBeAb(+ ) , HBcAb( +), and PreSl( + );Cgroup with more than 107 copies/ml, the common model is HBsAg( + ),HBeAg( + ) ,HBcAb( + ), and PreSl( + ). Conclusion There are very good correlation among three system of hepatitis B,Pre-S1 antigen and HBV-DNA level, they may reflect the replication of hepatitis B virus sensitively.