CAJ | 학술논문

背景:目前软骨支架材料种类繁多,随着制备工艺、结构及表面改性技术的提高,材料的性能更加完善,而一体化修复关节软骨及软骨下骨缺损对于软骨替代材料的稳定性十分重要.目的:观察新型生物复合材料聚氨酯/纳米羟基磷灰石+聚酰胺66一体化修复关节软骨及软骨下骨缺损的效果.方法:将多孔聚氨酯,纳米羟基磷灰石+聚酰胺66、致密聚氨酯,纳米羟基磷灰石+聚酰胺66及单纯纳米羟基磷灰石+聚酰胺66材料植入狗膝关节,空白组作为对照,分别于4,12,24周,对动物行大体观察,局部行组织学切片观察,对支架材料植入后24周修复组织进行组织学评分,扫描电镜观察生物材料与周边软骨连接界面的情况.结果与结论:支架材料植入后12,24周,下层材料中可见有骨组织长入,多孔聚氨酯与周边软骨融合较好,周边软骨未见明显退变.植入后24周,扫描电镜见上层材料多孔聚氨酯与周围正常软骨结合牢固,未见明显间隙,周围软骨未见明显退变;致密聚氨酯与周边正常软骨有较明显间隙,周边正常软骨退行性改变,变薄、卷曲.说明新型多孔聚氨酯/纳米羟基磷灰石+聚酰胺66支架材料在结构上更加接近正常软骨及软骨下骨,对软骨及软骨下骨缺损的修复效果更加显著.
배경:목전연골지가재료충류번다,수착제비공예、결구급표면개성기술적제고,재료적성능경가완선,이일체화수복관절연골급연골하골결손대우연골체대재료적은정성십분중요.목적:관찰신형생물복합재료취안지/납미간기린회석+취선알66일체화수복관절연골급연골하골결손적효과.방법:장다공취안지,납미간기린회석+취선알66、치밀취안지,납미간기린회석+취선알66급단순납미간기린회석+취선알66재료식입구슬관절,공백조작위대조,분별우4,12,24주,대동물행대체관찰,국부행조직학절편관찰,대지가재료식입후24주수복조직진행조직학평분,소묘전경관찰생물재료여주변연골련접계면적정황.결과여결론:지가재료식입후12,24주,하층재료중가견유골조직장입,다공취안지여주변연골융합교호,주변연골미견명현퇴변.식입후24주,소묘전경견상층재료다공취안지여주위정상연골결합뢰고,미견명현간극,주위연골미견명현퇴변;치밀취안지여주변정상연골유교명현간극,주변정상연골퇴행성개변,변박、권곡.설명신형다공취안지/납미간기린회석+취선알66지가재료재결구상경가접근정상연골급연골하골,대연골급연골하골결손적수복효과경가현저.
BACKGROUND: Cartilage stent materials were abundant. With the development of preparation technique, structure, and surface modification technique, performance of materials were improved remarkably; thus, Integrated-repaidng defects of articular cartilage and subchondral bone required high stability of cartilage replacement materials.OBJECTIVE: To evaluate the effect of polyurethane/nano-hydroxyapatite+polyamide66 (PU/n-HA+PA66) biological composite material on repairing articular cartilage and subchondral bone.METHODS: Twenty dog models were randomly divided into 4 groups: porous PU/n-HA+PA66 implant group, compact PU/n-HA+PA66 implant group, n-HA+PA66 implant group and blank control group. The macroscopic and histological evaluations were performed at 4, 12, and 24 weeks postoperatively. The interfaces of biomaterial implants with articular cartilage were observed by scanning electron microscope.RESULTS AND CONCLUSION: After 12 and 24 weeks, bone tissue grew into lower layer materials. Porous PU was well fused with peripheral cartilage. At 24 weeks postoperatively, the porous PU was closely connected with surrounding articular cartilage than the compact PU, and the articular cartilage surrounding the compact PU degenerated obviously. PU/n-HA+PA66 had a similar structure with normal cartilage and subchondral bone and had remarkable effects on repairing defects of articular cartilage and subchondral bone.