中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2011年
11期
928-931
,共4页
胰高血糖素样肽-2%梗阻性黄疸%肠道屏障功能
胰高血糖素樣肽-2%梗阻性黃疸%腸道屏障功能
이고혈당소양태-2%경조성황달%장도병장공능
Glucagon-like peptide-2%Obstructive jaundice%Barrier function
目的 探讨胰高血糖素样肽-2(GLP-2)对梗阻性黄疸模型大鼠肠道屏障功能的保护作用机制.方法 将72只SD大鼠随机分为3组:实验组(T组,n=24)、手术对照组(C组,n=24)和假手术组(SO组,n=24).T组和C组双重结扎胆总管,建立梗阻性黄疸模型；SO组开腹但不结扎胆总管.结扎后,T组腹腔注射GLP-2 (250 μg·kg-1·d-1),C组和SO组注射等体积0.01 mol/L的PBS溶液.于手术后第1、3、7天分别分批处死动物.应用RT-PCR技术半定量检测空肠黏膜GLP-2R mRNA的表达,应用免疫组织化学方法检测肠黏膜B细胞淋巴瘤/白血病基因-2(bcl-2)的表达.结果 T组肠黏膜GLP-2R mRNA的表达量比C组高,差异有统计学意义(P＜0.05),比SO组表达量稍低,差异无统计学意义(P＞0.05);C组肠黏膜bcl-2的表达于术后逐渐减少,差异有统计学意义(P＜0.05),且其表达量较SO组和T组少(P＜0.05),特别是第3、7天时,比SO组和T组降低更明显(P＜0.05).结论 GLP-2可以增加实验性梗阻性黄疸时肠黏膜细胞GLP-2R的表达,阻止肠黏膜细胞的凋亡,从而发挥保护肠道屏障功能的作用.
目的 探討胰高血糖素樣肽-2(GLP-2)對梗阻性黃疸模型大鼠腸道屏障功能的保護作用機製.方法 將72隻SD大鼠隨機分為3組:實驗組(T組,n=24)、手術對照組(C組,n=24)和假手術組(SO組,n=24).T組和C組雙重結扎膽總管,建立梗阻性黃疸模型；SO組開腹但不結扎膽總管.結扎後,T組腹腔註射GLP-2 (250 μg·kg-1·d-1),C組和SO組註射等體積0.01 mol/L的PBS溶液.于手術後第1、3、7天分彆分批處死動物.應用RT-PCR技術半定量檢測空腸黏膜GLP-2R mRNA的錶達,應用免疫組織化學方法檢測腸黏膜B細胞淋巴瘤/白血病基因-2(bcl-2)的錶達.結果 T組腸黏膜GLP-2R mRNA的錶達量比C組高,差異有統計學意義(P＜0.05),比SO組錶達量稍低,差異無統計學意義(P＞0.05);C組腸黏膜bcl-2的錶達于術後逐漸減少,差異有統計學意義(P＜0.05),且其錶達量較SO組和T組少(P＜0.05),特彆是第3、7天時,比SO組和T組降低更明顯(P＜0.05).結論 GLP-2可以增加實驗性梗阻性黃疸時腸黏膜細胞GLP-2R的錶達,阻止腸黏膜細胞的凋亡,從而髮揮保護腸道屏障功能的作用.
목적 탐토이고혈당소양태-2(GLP-2)대경조성황달모형대서장도병장공능적보호작용궤제.방법 장72지SD대서수궤분위3조:실험조(T조,n=24)、수술대조조(C조,n=24)화가수술조(SO조,n=24).T조화C조쌍중결찰담총관,건립경조성황달모형；SO조개복단불결찰담총관.결찰후,T조복강주사GLP-2 (250 μg·kg-1·d-1),C조화SO조주사등체적0.01 mol/L적PBS용액.우수술후제1、3、7천분별분비처사동물.응용RT-PCR기술반정량검측공장점막GLP-2R mRNA적표체,응용면역조직화학방법검측장점막B세포림파류/백혈병기인-2(bcl-2)적표체.결과 T조장점막GLP-2R mRNA적표체량비C조고,차이유통계학의의(P＜0.05),비SO조표체량초저,차이무통계학의의(P＞0.05);C조장점막bcl-2적표체우술후축점감소,차이유통계학의의(P＜0.05),차기표체량교SO조화T조소(P＜0.05),특별시제3、7천시,비SO조화T조강저경명현(P＜0.05).결론 GLP-2가이증가실험성경조성황달시장점막세포GLP-2R적표체,조지장점막세포적조망,종이발휘보호장도병장공능적작용.
Objective To investigate the effect of glucagon-like peptide-2(GLP-2) on intestinal barrier function in the bile duct ligated rats.Methods Seventy-two SD rats were randomly divided into three groups:GLP-2 treated group(T group),obstructive jaundice control group (C group) and sham operation group (SO group).The mRNA expression of GLP-2R was measured by semi-quantified reverse transcription polymerase chain reaction (RT-PCR)and the bcl-2 expression in the intestinal mucosa was measured by immunohistochemistry staining equipped image analyzing systems (Image proplus Version 4.5).Results The mRNA expression of GLP-2 in intestinal mucosa in T group was higher than that in C group (P＜0.05) but lower than that in SO group (P＞0.05).The expression of bcl-2 in the intestinal villi of rats in C group showed more significant decrease (P＜0.05) than those in the SO and T groups especially on day 3 and 7 after operation (P＜0.05).Conclusions GLP-2 may increase the mRNA expression of GLP-2R,stimulate the growth of intestinal mucosa,diminish the number of the apoptosis cells,and protect the intestinal barrier function in obstructive jaundice rats.
