中国医药
中國醫藥
중국의약
CHINA MEDICINE
2012年
6期
744-745
,共2页
凡德他尼%色谱法,高压液相%大鼠
凡德他尼%色譜法,高壓液相%大鼠
범덕타니%색보법,고압액상%대서
Vandetanib%Chromatography,high pressure liquid%Rat
目的 建立大鼠血浆中凡德他尼的高效液相色谱测定方法,为凡德他尼药代动力学研究提供参考.方法 血浆样品经处理后,采用高效液相色谱法进行分析.Agilent HC-C18柱分离,流动相采用乙腈-0.02 mol/L磷酸二氢钠溶液(80∶20),流速为1 ml/min；利用光电二极管阵列检测器对流份进行检测,柱温30℃.结果 内源性物质不干扰测定,凡德他尼的线性范围为0.5 ～ 10 mg/L(R =0.997),最低定量限值为0.5 mg/L,基质效应为95.2％～103.7％,加样回收率为73.1％ ～87.9％.结论 建立的高效液相色谱方法简便、灵敏、准确、所需样本量小,可为凡德他尼血药浓度检测提供依据.
目的 建立大鼠血漿中凡德他尼的高效液相色譜測定方法,為凡德他尼藥代動力學研究提供參攷.方法 血漿樣品經處理後,採用高效液相色譜法進行分析.Agilent HC-C18柱分離,流動相採用乙腈-0.02 mol/L燐痠二氫鈉溶液(80∶20),流速為1 ml/min；利用光電二極管陣列檢測器對流份進行檢測,柱溫30℃.結果 內源性物質不榦擾測定,凡德他尼的線性範圍為0.5 ～ 10 mg/L(R =0.997),最低定量限值為0.5 mg/L,基質效應為95.2％～103.7％,加樣迴收率為73.1％ ～87.9％.結論 建立的高效液相色譜方法簡便、靈敏、準確、所需樣本量小,可為凡德他尼血藥濃度檢測提供依據.
목적 건립대서혈장중범덕타니적고효액상색보측정방법,위범덕타니약대동역학연구제공삼고.방법 혈장양품경처리후,채용고효액상색보법진행분석.Agilent HC-C18주분리,류동상채용을정-0.02 mol/L린산이경납용액(80∶20),류속위1 ml/min；이용광전이겁관진렬검측기대류빈진행검측,주온30℃.결과 내원성물질불간우측정,범덕타니적선성범위위0.5 ～ 10 mg/L(R =0.997),최저정량한치위0.5 mg/L,기질효응위95.2％～103.7％,가양회수솔위73.1％ ～87.9％.결론 건립적고효액상색보방법간편、령민、준학、소수양본량소,가위범덕타니혈약농도검측제공의거.
Objective To develop a high performance liquid chromatography(HPLC)method for the determination of vandetanib in rat plasma.Methods The plasma samples were extracted and separated by an Agilent HC-C18 column,using methanol-0.02 mol/L sodium dihydrogen phosphate (80∶20 )as the mobile phase.The flow rate was 1.0 ml/min.The fractions were simultaneously detected by DAD with wavelength at 270 nm.Results The method was linear for vandetanib in the range of 0.5-10 mg/L.The correlation coefficient was 0.997 and the lower limit of quantification was 0.5 mg/L.The matrix effect was from 95.2％ to 103.7％.The extraction recovery was in the range of 73.1％-87.9％.Conclusions The HPLC method is simple,sensitive and accurate.It is suitable for the determination of vandetanib in rat plasma.
