中华泌尿外科杂志
中華泌尿外科雜誌
중화비뇨외과잡지
CHINESE JOURNAL OF UROLOGY
2010年
2期
88-91
,共4页
关晓峰%邓耀良%黎承扬%孙丙华
關曉峰%鄧耀良%黎承颺%孫丙華
관효봉%산요량%려승양%손병화
肾结石%高草酸尿症%巨噬细胞
腎結石%高草痠尿癥%巨噬細胞
신결석%고초산뇨증%거서세포
Kidney calculi%Hyperotreti%Macrophage
目的 探讨巨噬细胞转运肾结石晶体形成Randall斑的机制.方法 雄性SD鼠25只,随机分为5组,每组5只.A组为对照组,B～E组分别以含1%乙二醇和1%氯化铵的单蒸水加标准颗粒饲料喂养3、5、7、14 d建立高草酸尿症大鼠模型.收集各组大鼠24 h尿液,离子色谱法测定尿液草酸浓度.免疫组化法检测大鼠肾骨桥蛋白和巨噬细胞的表达,结果 以吸光度(A)值表示.透射电镜观察肾小管上皮细胞基底膜和间质处的巨噬细胞和结石晶体. 结果 A～E 5组大鼠尿液草酸浓度分别为(0.22±0.13)、(0.29±0.08)、(0.50±0.26)、(0.41±0.22)、(0.25±0.12)ng/ml;肾髓袢处骨桥蛋白的表达量分别为0.16±0.04、0.25±0.09、0.37±0.10、0.23±0.08、0.19±0.02,骨桥蛋白表达量与尿草酸浓度呈正相关(r=0.887,P<0.05).肾小管上皮细胞基底膜处巨噬细胞表达量分别为0.12±0.08、0.19±0.06、0.27±0.04、0.16±0.03、0.18±0.03,与骨桥蛋白表达量呈正相关(r=0.596,P<0.05).透射电镜下见巨噬细胞趋化至肾髓袢基底膜处,并裂解释放结石晶体.结论 巨噬细胞能在肾骨桥蛋白的趋化作用下将结石晶体转运至髓袢基底膜处,从而成为Randall斑的起源.
目的 探討巨噬細胞轉運腎結石晶體形成Randall斑的機製.方法 雄性SD鼠25隻,隨機分為5組,每組5隻.A組為對照組,B～E組分彆以含1%乙二醇和1%氯化銨的單蒸水加標準顆粒飼料餵養3、5、7、14 d建立高草痠尿癥大鼠模型.收集各組大鼠24 h尿液,離子色譜法測定尿液草痠濃度.免疫組化法檢測大鼠腎骨橋蛋白和巨噬細胞的錶達,結果 以吸光度(A)值錶示.透射電鏡觀察腎小管上皮細胞基底膜和間質處的巨噬細胞和結石晶體. 結果 A～E 5組大鼠尿液草痠濃度分彆為(0.22±0.13)、(0.29±0.08)、(0.50±0.26)、(0.41±0.22)、(0.25±0.12)ng/ml;腎髓袢處骨橋蛋白的錶達量分彆為0.16±0.04、0.25±0.09、0.37±0.10、0.23±0.08、0.19±0.02,骨橋蛋白錶達量與尿草痠濃度呈正相關(r=0.887,P<0.05).腎小管上皮細胞基底膜處巨噬細胞錶達量分彆為0.12±0.08、0.19±0.06、0.27±0.04、0.16±0.03、0.18±0.03,與骨橋蛋白錶達量呈正相關(r=0.596,P<0.05).透射電鏡下見巨噬細胞趨化至腎髓袢基底膜處,併裂解釋放結石晶體.結論 巨噬細胞能在腎骨橋蛋白的趨化作用下將結石晶體轉運至髓袢基底膜處,從而成為Randall斑的起源.
목적 탐토거서세포전운신결석정체형성Randall반적궤제.방법 웅성SD서25지,수궤분위5조,매조5지.A조위대조조,B～E조분별이함1%을이순화1%록화안적단증수가표준과립사료위양3、5、7、14 d건립고초산뇨증대서모형.수집각조대서24 h뇨액,리자색보법측정뇨액초산농도.면역조화법검측대서신골교단백화거서세포적표체,결과 이흡광도(A)치표시.투사전경관찰신소관상피세포기저막화간질처적거서세포화결석정체. 결과 A～E 5조대서뇨액초산농도분별위(0.22±0.13)、(0.29±0.08)、(0.50±0.26)、(0.41±0.22)、(0.25±0.12)ng/ml;신수번처골교단백적표체량분별위0.16±0.04、0.25±0.09、0.37±0.10、0.23±0.08、0.19±0.02,골교단백표체량여뇨초산농도정정상관(r=0.887,P<0.05).신소관상피세포기저막처거서세포표체량분별위0.12±0.08、0.19±0.06、0.27±0.04、0.16±0.03、0.18±0.03,여골교단백표체량정정상관(r=0.596,P<0.05).투사전경하견거서세포추화지신수번기저막처,병렬해석방결석정체.결론 거서세포능재신골교단백적추화작용하장결석정체전운지수번기저막처,종이성위Randall반적기원.
Objective To investigate the mechanisms of calculus crystal transport by macro-phage in kidney. Methods Hyperoxaluria rat model was established by administration of 1% ethyl-ene glycol and 1% ammonium chloride in drinking water. 24 h rat urine was collected, urinary oxalate were analyzed by ion chromatography. The expression and location of osteopontin and macrophage in kidney were observed by immunohistochemistry. Macrophage and calculus crystal at the basement membrane of renal tubular epithelial cells and interstitium were observed. Results The urinary ox-slate concentration were (0.22±0.13), (0.29±0.08), (0. 50±0.26), (0. 41±0. 22), (0.25±0. 12) ng/ml among these 5 groups. The osteoponitin expression was 0.16±0.04, 0.25±0.09, 0.37±0.10, 0.23±0.08, 0.19±0.02 respectively. The expression of osteopontin was positively correlated with urinary oxlate concentration(r=0.887, P<0.05). The macrophage at the basement membrane of renal tubular epithelial cells was 0.12±0.08, 0.19±0.06, 0.27±0.04, 0.16±0.03, 0.18±0.03 respectively. The macrophage distribution was positively correlated with the expression of osteopontin (r= 0.596, P<0.05). The macrophage moved from vessel to the basement membrane of loops of Henle, then disrupted and released the calculus crystal. Conclusions The macrophage might take part in the calculus crystal transport in kidney at the basement membrane of loops of Henle, which may be the source of Randall plaque. This process may be mediated by osteopontin.
