CAJ | 학술논문

目的了解云南省德宏州HIV-1新近感染者中耐药的发生情况.方法收集德宏州2006年7-12月期间所有的HIV-1阳性样品共1048份,使用BED捕获酶免疫试验进行新近感染检测,共检出新近感染样品101份;使用Bayer公司的TruGene方法进行基因型耐药检测,应用所有样品的序列构建系统进化树以确定样品的亚型.结果云南省德宏州HIV.1新近感染者巾的耐药发生率为6.25%(4/64),样品PR区中M36I/V、L63P和H69K等次要耐药突变出现的频率较高,分别占81.25%、70.31%和65.63%;HIV-1流行株以C/CRF07 BC/08_BC为主,占65.63%(42/64).结论德宏州HIV-1新近感染者中耐药的流行处于中等水平;有必要加强HIV-1的耐药监测.
목적 료해운남성덕굉주HIV-1신근감염자중내약적발생정황.방법 수집덕굉주2006년7-12월기간소유적HIV-1양성양품공1048빈,사용BED포획매면역시험진행신근감염검측,공검출신근감염양품101빈;사용Bayer공사적TruGene방법진행기인형내약검측,응용소유양품적서렬구건계통진화수이학정양품적아형.결과운남성덕굉주HIV.1신근감염자건적내약발생솔위6.25%(4/64),양품PR구중M36I/V、L63P화H69K등차요내약돌변출현적빈솔교고,분별점81.25%、70.31%화65.63%;HIV-1류행주이C/CRF07 BC/08_BC위주,점65.63%(42/64).결론덕굉주HIV-1신근감염자중내약적류행처우중등수평;유필요가강HIV-1적내약감측.
Objective To study the HIV-1 drug resistance (DR) situation among newly infected persons in Dehong.Methods 1048 HIV-1 positive blood samples from July to December in 2006 from Dehong prefecture of Yunnan,were collected.HIV drug resistance were tested using TruGene in newly infected people that were distinguished with BED-CEIA,while the subtype were determined with phylogenetic analysis using a set of reference sequences available on the Los Alamos Database.Results Of sixty-four successfully analyzed samples,drug resistance mutations were detected in 4 samples with the resistance rate as 6.25%.Minor mutation in PR region such as M36I/V,L63P and H69K appeared frequently and the rates were 81.25%,70.31%and 65.63%respectively.The predominantly prevalent strains were seen as C/CRF07_BC/08_BC(65.63%,42/64) in this study.Conclusion The prevalence of genotypic drug resistances in HIV-1 recent infections in Dehong prefecture appeared to be at moderate level.Drug-resistance surveillance program among HIV-1 infections should be continued and strengthened.