CAJ | 학술논문

目的探讨原发性胆汁性肝硬化(PBC)患者肝脏雌激素受体(ER-α)与外周血T淋巴细胞亚群细胞因子和自身抗体的相关性.方法采取横断面研究方法,设未治疗的80例女性PBC患者为PBC组;健康对照组为10例基线匹配的健康女性志愿者；非PBC对照组为20例非自身免疫性肝病患者,ELISA法检测各组外周细胞因子:白细胞介素6(IL-6)、IL-8、IL-22、肿瘤坏死因子α(TNFα)、干扰素γ(IFNY);自身抗体:M2型抗线粒体抗体(AMA-M2)、抗-Sp100及抗-gp210水平;免疫组织化学法检测PBC组及非PBC对照组肝组织ER-α的表达,指标相关性采用Spearman相关性检验,数值资料比较采用秩和检验.结果与健康对照组相比,PBC组和非PBC对照组的细胞因子水平显著升高;而PBC组和非PBC对照组比较,差异无统计学意义.PBC组肝组织ER-α阳性率为(50.7±8.9)％显著高于非PBC对照组(10.2±2.8)％,Z=4.82,P＜0.05,差异有统计学意义.PBC组肝组织ER-α阳性率与血清AMA-M2、抗-SP100、抗-gp210的阳性率呈正相关,相关系数r分别为0.898、0.819和0.814,P值均＜0.05,差异有统计学意义;ER-α与血清细胞因子IL-22、TNFα、IFNγ的含量也呈正相关,相关系数r分别为0.71、0.89和0.82,P＜0.05,差异有统计学意义.非PBC对照组血清AMA-M2、抗-Sp100、抗-gp210为阴性,肝组织ER-α表达与血清细胞因子水平无相关性(P＞0.05).结论 PBC患者血清细胞因子水平和ER-α阳性率显著升高,与自身抗体阳性率呈正相关,提示PBC患者肝组织高表达ER-α,可能是PBC的发病原因之一.
목적 탐토원발성담즙성간경화(PBC)환자간장자격소수체(ER-α)여외주혈T림파세포아군세포인자화자신항체적상관성.방법 채취횡단면연구방법,설미치료적80례녀성PBC환자위PBC조;건강대조조위10례기선필배적건강녀성지원자；비PBC대조조위20례비자신면역성간병환자,ELISA법검측각조외주세포인자:백세포개소6(IL-6)、IL-8、IL-22、종류배사인자α(TNFα)、간우소γ(IFNY);자신항체:M2형항선립체항체(AMA-M2)、항-Sp100급항-gp210수평;면역조직화학법검측PBC조급비PBC대조조간조직ER-α적표체,지표상관성채용Spearman상관성검험,수치자료비교채용질화검험.결과 여건강대조조상비,PBC조화비PBC대조조적세포인자수평현저승고;이PBC조화비PBC대조조비교,차이무통계학의의.PBC조간조직ER-α양성솔위(50.7±8.9)％현저고우비PBC대조조(10.2±2.8)％,Z=4.82,P＜0.05,차이유통계학의의.PBC조간조직ER-α양성솔여혈청AMA-M2、항-SP100、항-gp210적양성솔정정상관,상관계수r분별위0.898、0.819화0.814,P치균＜0.05,차이유통계학의의;ER-α여혈청세포인자IL-22、TNFα、IFNγ적함량야정정상관,상관계수r분별위0.71、0.89화0.82,P＜0.05,차이유통계학의의.비PBC대조조혈청AMA-M2、항-Sp100、항-gp210위음성,간조직ER-α표체여혈청세포인자수평무상관성(P＞0.05).결론 PBC환자혈청세포인자수평화ER-α양성솔현저승고,여자신항체양성솔정정상관,제시PBC환자간조직고표체ER-α,가능시PBC적발병원인지일.
Objective To investigate the correlation between ER-α in the liver and cytokines of T lymphocytes subsets and serum signatures in PBC patients.Methods The research is performed with crosssectional study.80 PBC women patients without treatment were enrolled in PBC group,10 healthy women as baseline-matched in healthy-control group,and 20 patients with non-autoimmune liver disease in non-PBC control group.The expression of IL-6,IL-8,IL-22,TNFα,IFNγ,AMA-M2,Sp100 and gp210 were analyzed in Peripheral Blood using ELISA in all groups,and ER-α of patients were performed on tissues from liver biopsies in PBC gruop and non-PBC control group with immunohistochemistry.Spearman correlation test were performed on the indices to identified the association of all Parameters.numerical data were compared with Wilcoxon rank-sum test.Results Compared with healthy-control group,expression of serum cytokines are significantly higher in PBC and non-PBC groups (P＜0.01),while no significant difference were observed between PBC and non-PBC groups.The positive rate of ER-α in PBC patients liver tissues in PBC group is higher than that in non-PBC group (Z=4.82,P＜0.01).Expression of ER-α is positively correlated with positive rates of AMA-M2 antibody,Sp100 and gP210 of tissues of PBC patients (r=0.898,0.819,0.814,P＜0.01).ER-a is positive correlated with the expression of cytokines,among which the coefficient of correlation of IL-22,TNFα,IFNγ is more than 0.7(r=0.71,0.89,0.82,P＜0.01),AMA-M2,Sp100,gp210 is negative in serum of non-PBC control group.No obviously correlations were indicated between the expression of ER-α and cytokines.Conclusion A high level of expression of cytokines in the serum might be one of the factors of etiopathogensis of PBC.