中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
7期
1217-1220
,共4页
吴立生%吴仕峰%高万里%裴保安%宋炎成%李延志
吳立生%吳仕峰%高萬裏%裴保安%宋炎成%李延誌
오립생%오사봉%고만리%배보안%송염성%리연지
嗅鞘细胞%骨髓基质干细胞%脊髓损伤%联合移植
嗅鞘細胞%骨髓基質榦細胞%脊髓損傷%聯閤移植
후초세포%골수기질간세포%척수손상%연합이식
Olfactory ensheathing cells%Bone marrow mesenchymal stem cells%Spinal cord injury%Co-transplantation
目的 探讨嗅鞘细胞(OECs)和骨髓基质干细胞(BMSCs)联合移植修复脊髓损伤的疗效.方法 采用改良Allen法,制备大鼠脊髓损伤模型,随机分成5组:手术对照组、正常对照组、单细胞移植组(OECs或BMSCs)和联合移植组.将联合培养的OECs和BMSCs移植到脊髓完全损伤的大鼠体内.评定功能恢复状况,采用荧光技术对标记后的脊髓进行示踪.结果 OECs和BMSCs移植后均可在体内存活.移植4周后与手术对照组(0.88±0.23)比较,OECs组(2.67±0.24)和BMSCs组(2.66±0.14)的大鼠脊髓功能均有改善,联合移植组(3.12±0.52)功能改善最佳(P＜0.01).结论 OECs与BMSCs在体外可联合培养,OECs的培养液对BMSCs具有向神经元分化的趋化作用,并且两者在移植后均可存活,联合移植较单细胞移植的修复作用更佳.
目的 探討嗅鞘細胞(OECs)和骨髓基質榦細胞(BMSCs)聯閤移植脩複脊髓損傷的療效.方法 採用改良Allen法,製備大鼠脊髓損傷模型,隨機分成5組:手術對照組、正常對照組、單細胞移植組(OECs或BMSCs)和聯閤移植組.將聯閤培養的OECs和BMSCs移植到脊髓完全損傷的大鼠體內.評定功能恢複狀況,採用熒光技術對標記後的脊髓進行示蹤.結果 OECs和BMSCs移植後均可在體內存活.移植4週後與手術對照組(0.88±0.23)比較,OECs組(2.67±0.24)和BMSCs組(2.66±0.14)的大鼠脊髓功能均有改善,聯閤移植組(3.12±0.52)功能改善最佳(P＜0.01).結論 OECs與BMSCs在體外可聯閤培養,OECs的培養液對BMSCs具有嚮神經元分化的趨化作用,併且兩者在移植後均可存活,聯閤移植較單細胞移植的脩複作用更佳.
목적 탐토후초세포(OECs)화골수기질간세포(BMSCs)연합이식수복척수손상적료효.방법 채용개량Allen법,제비대서척수손상모형,수궤분성5조:수술대조조、정상대조조、단세포이식조(OECs혹BMSCs)화연합이식조.장연합배양적OECs화BMSCs이식도척수완전손상적대서체내.평정공능회복상황,채용형광기술대표기후적척수진행시종.결과 OECs화BMSCs이식후균가재체내존활.이식4주후여수술대조조(0.88±0.23)비교,OECs조(2.67±0.24)화BMSCs조(2.66±0.14)적대서척수공능균유개선,연합이식조(3.12±0.52)공능개선최가(P＜0.01).결론 OECs여BMSCs재체외가연합배양,OECs적배양액대BMSCs구유향신경원분화적추화작용,병차량자재이식후균가존활,연합이식교단세포이식적수복작용경가.
Objective To investigate effects of co-transplantation of olfactory ensheathing cells (OECs) and bone marrow mesenchymal stem cells (BMSCs) for repair of the injured spine cord.Methods The models of spinal cord injury was established by using the modified Allen method,and randomly divided into 5 groups,including sham operation group,normal control group,OECs or BMScs transplantation group and co-transplantation group.The function recovery was evaluated,and the labeled spine was traced by using fluorescent technique.Results OECs and BMSCs survived after transplantation.At 4th week after transplantation,as compared with sham operation group (0.88 ±0.23),the function of rat spine cord was improved in OECs transplantation group (2.67 ±0.24) or BMSCs transplantation group (2.66 ±0.14),most significantly in the co-transplantation group ( 3.12 ± O.52 ) ( P＜0.01 ).Conclusion It was more significant to repair rat spine cord injure by co-transplantation of OECs and BMSCs than transplantation of OECs or BMSCs alone.
