CAJ | 학술논문

目的利用TEM观察经Nonidet P-40处理的A型流感病毒表面形态变化,研究不同表面活性剂-病毒表面相互作用情况,以提供一种较为温和的病毒表面裂解条件,并考察表面活性剂存在条件下对病毒表面抗体结合作用的影响,为利用病毒内部抗体结合病毒下层结构提供免疫诊断学理论基础.方法通过用不同浓度的非离子型表面活性剂NP-40对完整的A型流感病毒进行处理,经透射电子显微镜成像,观察两种试剂对病毒表面形态的破坏程度,并考察病毒抗体结合情况.结果 NP-40各浓度对病毒表面破坏程度不一,病毒随浓度增高降解程度增大,但在0.1%NP-40及以下浓度上对抗体结合作用影响不大.结论通过表面活性剂优化处理病毒颗粒,对抗体的结合会产生影响,本研究为利用流感病毒内层抗体结合包膜病毒奠定了样本处理的理论基础.
목적 이용TEM관찰경Nonidet P-40처리적A형류감병독표면형태변화,연구불동표면활성제-병독표면상호작용정황,이제공일충교위온화적병독표면렬해조건,병고찰표면활성제존재조건하대병독표면항체결합작용적영향,위이용병독내부항체결합병독하층결구제공면역진단학이론기출.방법 통과용불동농도적비리자형표면활성제NP-40대완정적A형류감병독진행처리,경투사전자현미경성상,관찰량충시제대병독표면형태적파배정도,병고찰병독항체결합정황.결과 NP-40각농도대병독표면파배정도불일,병독수농도증고강해정도증대,단재0.1%NP-40급이하농도상대항체결합작용영향불대.결론 통과표면활성제우화처리병독과립,대항체적결합회산생영향,본연구위이용류감병독내층항체결합포막병독전정료양본처리적이론기출.
Objective Through observing the morphological changes of the prepared influenza viruses (H1N1) treated with the different concentration of Nonidet P-40 solutions and added with antibody against the influenza virus using transmission electron microscopy (TEM), to explore the principle of application of the internal antibody for immunoassay of influenza virus. Methods Through treating the virus samples with serial diluted Nonidet P-40 solutions from 0.01% to 0. 2% and/or not adding antibody against the influenza virus, then investigating the samples by TEM to obtain the morphological changes of the virions. Results The serial images show that the denudation degree of the virions is proportional with the rise of NP-40 concentration, and partly denuded virion image appeared at 0. 1% NP-40 treatment, also under this condition no influence was observed on antibody binding to the virus. Conclusion This work demonstrated the interaction between influenza virion and its antibody under nonionic surfactants existing,which supports the advantages of sample preparation for immunoassay enveloped virus using internal antibody theoretically.