CAJ | 학술논문

目的探讨人眼巩膜组织中亮蛋白聚糖基因mRNA水平与高度近视及青光眼的相关性.方法 2009年10月至2010年12月在首都医科大学附属同仁医院住院行抗青光眼手术的原发性开角型青光眼患者21例及自愿捐献角膜的健康成年人12例(正视眼).11例行抗青光眼手术的原发性开角型青光眼患者(不伴有高度近视)及10例行抗青光眼手术的原发性开角型青光眼患者(伴有高度近视)术中切除的眼前节巩膜组织.提取巩膜组织中总RNA,针对亮蛋白聚糖基因设计特异性引物进行逆转录多聚酶链反应(RT-PCR)检查,用凝胶分析系统测定各样本β肌动蛋白和亮蛋白聚糖基因PCR反应产物电泳条带灰度值,采用单因素方差分析进行比值(β肌动蛋白电泳条带灰度值/亮蛋白聚糖电泳条带灰度值)的统计分析.结果 β肌动蛋白/亮蛋白聚糖比值在青光眼伴高度近视组[0.29±0.10]和青光眼组[0.29±0.10]之间比较,差异无统计学意义(P＞0.05),而与正常对照组[2.67±1.05]比较,差异有统计学意义(P＜0.01).结论高度近视候选基因亮蛋白聚糖与高度近视可能不相关,但可能与青光眼存在一定相关性,尚有待进一步研究.
목적 탐토인안공막조직중량단백취당기인mRNA수평여고도근시급청광안적상관성.방법 2009년10월지2010년12월재수도의과대학부속동인의원주원행항청광안수술적원발성개각형청광안환자21례급자원연헌각막적건강성년인12례(정시안).11례행항청광안수술적원발성개각형청광안환자(불반유고도근시)급10례행항청광안수술적원발성개각형청광안환자(반유고도근시)술중절제적안전절공막조직.제취공막조직중총RNA,침대량단백취당기인설계특이성인물진행역전록다취매련반응(RT-PCR)검사,용응효분석계통측정각양본β기동단백화량단백취당기인PCR반응산물전영조대회도치,채용단인소방차분석진행비치(β기동단백전영조대회도치/량단백취당전영조대회도치)적통계분석.결과 β기동단백/량단백취당비치재청광안반고도근시조[0.29±0.10]화청광안조[0.29±0.10]지간비교,차이무통계학의의(P＞0.05),이여정상대조조[2.67±1.05]비교,차이유통계학의의(P＜0.01).결론 고도근시후선기인량단백취당여고도근시가능불상관,단가능여청광안존재일정상관성,상유대진일보연구.
Objective To identify differentially expressed lumcian in scleral tissue of eyes with primary open-angle glaucoma (POAG) and high mopia (HM).Methods Total RNA was isolated from scleral tissue of cadaveric eyes derived from normal donors,patient's eyes with diagnosed glaucoma and high mopia who accepted trabeculectomy. RNA was amplified,RT-PCR was used to measure the levels of mRNA.The ratio of the electrophoresis strips'gray scale values of the β-actin over the lumican gene was obtained for ANOVA analysis.Results β-actin/LUM of normal eye was significantly higher than that of POAG and POAG + HM eyes (P ＜ 0.01 ),but there was no significant difference between POAG and POAG + HM eyes (P ＞ 0.05).Conclusion Differentially expressed lumican between POAG and control groups identified in this study have not been previously investigated for their role in the pathogenesis of POAG and thus are novel factors for further study of the mechanism of the disease and for their possible use as diagnostic markers.