CAJ | 학술논문

摘要采用均匀设计安排实验，考察各影响因素与一组色谱分离评价函数间的综合关联程度。将关联度分析用于RP-HPLC同时测定多种头孢菌素的析因试验，从6个影响因素中确定主要影响因素。结果表明，乙腈配比、pH值、磷酸盐缓冲液浓度为主要影响因素。RP-HPLC同时测定头孢菌素时，关联度分析能更科学地确定色谱条件中的优化因子。
적요채용균균설계안배실험，고찰각영향인소여일조색보분리평개함수간적종합관련정도。장관련도분석용우RP-HPLC동시측정다충두포균소적석인시험，종6개영향인소중학정주요영향인소。결과표명，을정배비、pH치、린산염완충액농도위주요영향인소。RP-HPLC동시측정두포균소시，관련도분석능경과학지학정색보조건중적우화인자。
Correspondence analysis was used for determining the important factors from the six factors influencing analysis of cephalosporin in reversed phase-high performance liquid chromatography (RP-HPLC). Experiments were planed by U7（76）, then the synthetical correspondence between a series of optimizations and the factors was calculated. Result indicated that that proportion of organic solvent, pH and concentration of buffer were three most important factors. It showed that the correspondence analysis was a better method to determining the important factors in RP-HPLC