CAJ | 학술논문

目的探讨细胞色素P450(CYP1A1)基因异亮氨酸(Ile)-缬氨酸(Val)位点和Msp1位点多态性和肺癌易感性的相关关系.方法以病例对照的研究方法,采用PCR-RFLP和ASA-PCR技术检测82例原发性肺癌和91例对照的CYP1A1基因Ile-Val位点和Msp1位点多态性.结果Ile-Val三种多态基因型在肺癌组和对照组分布差异有显著性(P<0.05),Ile/Val、Val/Val基因型在肺癌组的分布频率明显高于对照组;logistic回归分析结果显示Ile/Val、Val/Val基因型患肺癌的危险分别是Ile/Ile基因型的1.969倍和3.150倍;当按吸烟分层后(将Ile/Val、Val/Val基因型合并分析),吸烟组中Ile/Val、Val/Val合并基因型患肺癌的危险是Ile/Ile基因型的3.059倍,而在不吸烟组其OR为1.687;Msp1位点多态性在肺癌组和对照组差异无统计学意义.结论 CYP1A1第7外显子的Ile/Val、Val/Val基因型与肺癌的易感性有关,可望作为肺癌易感人群筛选的重要指标;尚不能认为Msp1多态性与肺癌的易感性有关.
목적탐토세포색소P450(CYP1A1)기인이량안산(Ile)-힐안산(Val)위점화Msp1위점다태성화폐암역감성적상관관계.방법이병례대조적연구방법,채용PCR-RFLP화ASA-PCR기술검측82례원발성폐암화91례대조적CYP1A1기인Ile-Val위점화Msp1위점다태성.결과Ile-Val삼충다태기인형재폐암조화대조조분포차이유현저성(P<0.05),Ile/Val、Val/Val기인형재폐암조적분포빈솔명현고우대조조;logistic회귀분석결과현시Ile/Val、Val/Val기인형환폐암적위험분별시Ile/Ile기인형적1.969배화3.150배;당안흡연분층후(장Ile/Val、Val/Val기인형합병분석),흡연조중Ile/Val、Val/Val합병기인형환폐암적위험시Ile/Ile기인형적3.059배,이재불흡연조기OR위1.687;Msp1위점다태성재폐암조화대조조차이무통계학의의.결론 CYP1A1제7외현자적Ile/Val、Val/Val기인형여폐암적역감성유관,가망작위폐암역감인군사선적중요지표;상불능인위Msp1다태성여폐암적역감성유관.