CAJ | 학술논문

Objective: To clone and analyze the structure of a novel gene, named EST-1 (endoplasmic reticulum-localized seven-span transmembrane protein-1) and to analyze the expression pattern and intracellular location of EST-1. Methods: The cDNA library was screened to isolate novel cDNA fragment. The structure of novel gene was analysed by computer software. Expression of EST-1 was analyzed by dot blot and Northern blotting. Intracellular localization was observed after EST-1-enhanced green fluorescence protein (EGFP) fusion gene was transfected into mammalian cells. Results: The full-length cDNA of mouse EST-1 was 1 802 bp, with a 1 293 bp open reading frame encoding 431 amino acids. It was predicated that protein encoded by EST-1 contained a signal peptide sequence at the N-terminus, seven putative transmembrane domains, and an ER-retaining signal at the C-terminus. EST-1-EGFP fusion protein showed an ER-like intracellular distribution in mammalian cells. Expression pattern analysis showed that EST-1 is expressed in all tissues examined. Conclusion: EST-1 is encoding a putative seven-span transmembrane protein localized in endoplasmic reticulum. EST-1 was expressed in all tissues examined, suggesting an essential function of EST-1 in cells.