中国临床康复
中國臨床康複
중국림상강복
CHINESE JOURNAL OF CLINICAL REHABILITATION
2005年
16期
254-256
,共3页
GM1 神经节苷酯%颅脑照射%学习障碍%记忆障碍%迷宫学习
GM1 神經節苷酯%顱腦照射%學習障礙%記憶障礙%迷宮學習
GM1 신경절감지%로뇌조사%학습장애%기억장애%미궁학습
背景:记忆力下降是放射性脑病早期的主要临床表现,已有许多人认为神经节苷酯在神经修复中对记忆功能具有改善作用.目的:研究神经节苷酯对鼠脑放射性损伤后空间学习记忆能力减退的影响.设计:以动物为观察对象的随机对照实验.单位:中山大学第二附属医院的神经科和放射科.材料:实验于2001-03/2002-05在中山大学附属第二医院实验室完成.选取SD大鼠80只,分为空白对照组、神经节苷酯治疗组、生理盐水治疗组、未干预组,20只/组.干预:神经节苷酯治疗组、生理盐水治疗组、未干预组大鼠麻醉后头部接受60Coγ射线照射,7 Gy/次,1次/d,连续照射6 d,总剂量42 Gy.空白对照组麻醉后不予照射.接受照射的3组每天照射后隔1 h给药,神经节苷酯治疗组腹腔注射神经节苷酯30 mg/kg;生理盐水治疗组注射等量的生理盐水,1次/d,连续6 d;空白对照组和未干预组不给药.评估:①照射结束后采用Morris水迷宫的定位航行实验,通过记录大鼠寻找平台所需时间(潜伏期)来测定大鼠对水迷宫的学习记忆能力.②采用空间搜索实验,通过记录大鼠在120s内搜索平台的路线图,测量其平台象限的游泳距离占总距离的百分比,从而测定大鼠学会寻找平台后,对平台空间位置记忆的能力.③迷宫试验结束后将神经节苷酯治疗组、生理盐水治疗组、未干预组大鼠断头取脑,观察各组大鼠的脑组织病理改变.结果:①各组第4天起潜伏期渐趋平稳,第5天时神经节苷酯治疗组寻找平台潜伏期(13.6±1.4)s,短于生理盐水治疗组和未干预组[(17.1±2.9),(15.8±2.2)s,P＜O.05].②神经节苷酯治疗组和空白对照组能依靠空间搜索寻找平台,运动轨迹多位于原平台所在象限,而生理盐水治疗组及未干预组多绕池壁游泳,运动轨迹呈随机分布于各象限中.神经节苷酯治疗组平台象限游泳距离百分比高于生理盐水治疗组及未干预组,但较空白对照组低.③组织学检查显示,生理盐水治疗组神经元轻度变性,部分细胞呈空泡变性,出现细胞皱缩,染色质浓缩,核边聚,星形细胞数量减少;未干预组病理改变与生理盐水治疗组基本相似;神经节苷酯治疗组部分神经元出现胞体变小,胞浆红染等变性改变,但数量、核皱缩、核边聚、空泡现象较前两组少.结论:放射性脑损伤使大鼠的学习记忆能力明显下降,神经节苷酯治疗可减轻照射后脑组织病理改变,对放射所致的空间学习记忆能力减退有明显改善作用.
揹景:記憶力下降是放射性腦病早期的主要臨床錶現,已有許多人認為神經節苷酯在神經脩複中對記憶功能具有改善作用.目的:研究神經節苷酯對鼠腦放射性損傷後空間學習記憶能力減退的影響.設計:以動物為觀察對象的隨機對照實驗.單位:中山大學第二附屬醫院的神經科和放射科.材料:實驗于2001-03/2002-05在中山大學附屬第二醫院實驗室完成.選取SD大鼠80隻,分為空白對照組、神經節苷酯治療組、生理鹽水治療組、未榦預組,20隻/組.榦預:神經節苷酯治療組、生理鹽水治療組、未榦預組大鼠痳醉後頭部接受60Coγ射線照射,7 Gy/次,1次/d,連續照射6 d,總劑量42 Gy.空白對照組痳醉後不予照射.接受照射的3組每天照射後隔1 h給藥,神經節苷酯治療組腹腔註射神經節苷酯30 mg/kg;生理鹽水治療組註射等量的生理鹽水,1次/d,連續6 d;空白對照組和未榦預組不給藥.評估:①照射結束後採用Morris水迷宮的定位航行實驗,通過記錄大鼠尋找平檯所需時間(潛伏期)來測定大鼠對水迷宮的學習記憶能力.②採用空間搜索實驗,通過記錄大鼠在120s內搜索平檯的路線圖,測量其平檯象限的遊泳距離佔總距離的百分比,從而測定大鼠學會尋找平檯後,對平檯空間位置記憶的能力.③迷宮試驗結束後將神經節苷酯治療組、生理鹽水治療組、未榦預組大鼠斷頭取腦,觀察各組大鼠的腦組織病理改變.結果:①各組第4天起潛伏期漸趨平穩,第5天時神經節苷酯治療組尋找平檯潛伏期(13.6±1.4)s,短于生理鹽水治療組和未榦預組[(17.1±2.9),(15.8±2.2)s,P＜O.05].②神經節苷酯治療組和空白對照組能依靠空間搜索尋找平檯,運動軌跡多位于原平檯所在象限,而生理鹽水治療組及未榦預組多繞池壁遊泳,運動軌跡呈隨機分佈于各象限中.神經節苷酯治療組平檯象限遊泳距離百分比高于生理鹽水治療組及未榦預組,但較空白對照組低.③組織學檢查顯示,生理鹽水治療組神經元輕度變性,部分細胞呈空泡變性,齣現細胞皺縮,染色質濃縮,覈邊聚,星形細胞數量減少;未榦預組病理改變與生理鹽水治療組基本相似;神經節苷酯治療組部分神經元齣現胞體變小,胞漿紅染等變性改變,但數量、覈皺縮、覈邊聚、空泡現象較前兩組少.結論:放射性腦損傷使大鼠的學習記憶能力明顯下降,神經節苷酯治療可減輕照射後腦組織病理改變,對放射所緻的空間學習記憶能力減退有明顯改善作用.
배경:기억력하강시방사성뇌병조기적주요림상표현,이유허다인인위신경절감지재신경수복중대기억공능구유개선작용.목적:연구신경절감지대서뇌방사성손상후공간학습기억능력감퇴적영향.설계:이동물위관찰대상적수궤대조실험.단위:중산대학제이부속의원적신경과화방사과.재료:실험우2001-03/2002-05재중산대학부속제이의원실험실완성.선취SD대서80지,분위공백대조조、신경절감지치료조、생리염수치료조、미간예조,20지/조.간예:신경절감지치료조、생리염수치료조、미간예조대서마취후두부접수60Coγ사선조사,7 Gy/차,1차/d,련속조사6 d,총제량42 Gy.공백대조조마취후불여조사.접수조사적3조매천조사후격1 h급약,신경절감지치료조복강주사신경절감지30 mg/kg;생리염수치료조주사등량적생리염수,1차/d,련속6 d;공백대조조화미간예조불급약.평고:①조사결속후채용Morris수미궁적정위항행실험,통과기록대서심조평태소수시간(잠복기)래측정대서대수미궁적학습기억능력.②채용공간수색실험,통과기록대서재120s내수색평태적로선도,측량기평태상한적유영거리점총거리적백분비,종이측정대서학회심조평태후,대평태공간위치기억적능력.③미궁시험결속후장신경절감지치료조、생리염수치료조、미간예조대서단두취뇌,관찰각조대서적뇌조직병리개변.결과:①각조제4천기잠복기점추평은,제5천시신경절감지치료조심조평태잠복기(13.6±1.4)s,단우생리염수치료조화미간예조[(17.1±2.9),(15.8±2.2)s,P＜O.05].②신경절감지치료조화공백대조조능의고공간수색심조평태,운동궤적다위우원평태소재상한,이생리염수치료조급미간예조다요지벽유영,운동궤적정수궤분포우각상한중.신경절감지치료조평태상한유영거리백분비고우생리염수치료조급미간예조,단교공백대조조저.③조직학검사현시,생리염수치료조신경원경도변성,부분세포정공포변성,출현세포추축,염색질농축,핵변취,성형세포수량감소;미간예조병리개변여생리염수치료조기본상사;신경절감지치료조부분신경원출현포체변소,포장홍염등변성개변,단수량、핵추축、핵변취、공포현상교전량조소.결론:방사성뇌손상사대서적학습기억능력명현하강,신경절감지치료가감경조사후뇌조직병리개변,대방사소치적공간학습기억능력감퇴유명현개선작용.
BACKGROUND: Memory loss is the main presentation during the earlier stage of radiative eneephalopathy, and it was reported that ganglioside (GM1) played important role in neural rehabilitation, particular in the improvement of memory.OBJECTIVE: To study the improving effect of GM1 on spatial learning and memory retardation in rats following radiative encepholopathy. DESIGN: Randomized control and comparative observing study based on the experimental animals.SETTING: Department of Neurology and Department of Radiation of Second Affiliated Hospital of Sun Yat-sen University.MATERIALS: This study was carried out at the Laboratory of the Second Hospital Affiliated to Sun Yat-sen University between March 2001 and May 2002. Tctally 80 SD rats were randomly selected and divided into control group, GM1 treatment group, physiological saline group and non-intervention group with 20 rats in each group.INTERVENTIONS: Rats in GM1 group, physiological saline group and non-intervention group subjected to head 60Coγ irradiation of 7Gy each time after anesthesia, once a day for consecutive 6 days, and the total dosage was 42Gy while rats in control group did not receive irradiation after anesthesia. Rats in GM1 and physiological saline(PS) group were given intraperitoneal injection of GM1 and physiological saline of 30 mg/kg respectively at 1 hour after each time of radiation, once a day for consecutive 6 days but not in control group and non-intervention group. Evaluation: ①After irradiation, morris water labyrinth navigation test was used to assess the capability of learning and memory of rats by the time for reaching platform (latency); ② Spatial searching test was used to detect their spatial memory after learning how to reach the platform by recording the way of rats searching the platform in 120 s and calculating the percentage of swimming distance in platform quadrant in the total distance; ③ After labyrinth test, brains were taken out of the rats in GM1 group, PS group and non-intervention group for observing the histological and pathological changes in rat brains.RESULTS: ① The latency become stable form onset of the 4th day in each group. On the 5th day, the searching platform latency in GM1 group was(13.6±1.4) s, shorter than(17.1±2.9) s of PS group and [(15.8±2.2) s, (P＜0.05)] of non-intervention group; ② Rats in GM 1 and control group were found capable of searching platform according to their spatial memory, presented by swimming trail most located in platform quadrant while rats in PS and non-intervention groups were found mostly swimming around the pool with moving trails distributed randomly. The percentage of swimming distance in platform quadrant was found higher in GM 1 treatment group than in the PS group and non-intervention group, but lower than that in the control group; ③ Histological examination revealed slight neuronal degeneration in PS group, part of which was changes of vacuolar degeneration with cell shrank, chromosome concentrated and nuclei gathered aside, and the number of astrocytes also decreased; the pathological changes in non-intervention group and PS group were similar; in GM1 group, part neurons became smaller with peripalsm turning red but the pathological changes, such as the number of cells,neuclei shrank and gathered aside, and vacuolar changes were less than those of the former two groups.ONCLUSION: Radiative encephalopathy would result in obvious learning nd memory impairments in rats but histological and pathological changes due o brain radiation injury can be attenuated with the treatment of GM1, implying that GM1 may play important role in the improvement of radiation-induced spatial learning and memory loss.
