CAJ | 학술논문

对多年生黑麦草进行冷胁迫处理后,提取叶片总RNA,采用RT-PCR法获得了LpAFP成熟蛋白的cDNA克隆.序列分析结果表明,该成熟蛋白的开放读码框(ORF)长度为357bp,编码一条由118个氨基酸组成的多肽.生物信息学分析结果表明,黑麦草成熟抗冻蛋白与其他植物抗冻蛋白的同源性较低,与鱼类抗冻蛋白和昆虫抗冻蛋白则没有同源性.
대다년생흑맥초진행랭협박처리후,제취협편총RNA,채용RT-PCR법획득료LpAFP성숙단백적cDNA극륭.서렬분석결과표명,해성숙단백적개방독마광(ORF)장도위357bp,편마일조유118개안기산조성적다태.생물신식학분석결과표명,흑맥초성숙항동단백여기타식물항동단백적동원성교저,여어류항동단백화곤충항동단백칙몰유동원성.
Ryegrass (Lolium perenne) is an evergreen grass with strong cold resistance due to high active antifreeze protein' (LpAFP). To clone the sequences of LpAFP, the total RNA of perennial ryegrass treated with cold stress was extracted. Then, the cDNA of mature LpAFP was obtained by RT-PCR. The sequence analysis result show that mature LpAFP contained 357 bp, which encoded 118 amino acids. The result of bioinformatics analysis reveales that ryegrass antifreeze protein has low homology to other plant antifreeze proteins, and no homology to fish antifreeze proteins and insect antifreeze proteins.