CAJ | 학술논문

针对胜利油田孤岛中一区Ng3地层水的性质设计激活剂配方,在物理模拟条件下有效激活内源微生物,同时将硫酸盐还原菌浓度控制在50个/mL以下.微生物经激活后代谢活跃,产乙酸达346 mg/L,产出气体中甲烷含量5%.通过变性梯度凝胶电泳及其条带测序,定性分析了激活后内源微生物群落中的15个不同内源茵属,其中以嗜热厌氧茵为主,部分种属代谢产生的低分子有机酸、CO_2和H_2等,被其它细菌或产甲烷古茵作为代谢底物而利用,这些微生物组成了物理模拟实验条件下的完整代谢体系.
침대성리유전고도중일구Ng3지층수적성질설계격활제배방,재물리모의조건하유효격활내원미생물,동시장류산염환원균농도공제재50개/mL이하.미생물경격활후대사활약,산을산체346 mg/L,산출기체중갑완함량5%.통과변성제도응효전영급기조대측서,정성분석료격활후내원미생물군락중적15개불동내원인속,기중이기열염양인위주,부분충속대사산생적저분자유궤산、CO_2화H_2등,피기타세균혹산갑완고인작위대사저물이이용,저사미생물조성료물리모의실험조건하적완정대사체계.
Activating nutrients was designed for Zhongyi block in Shengli Oil Field according to the character of stratal water, which could stimulate indigenous microorganisms effectively, and keep Sulfate Reducing Bacteria under 50 cells·mL~(-1), where acetate and methane produced by microbe were 346 mg·L~(-1) and 5%, respectively. 15 different genuses of indigenous microorganisms were detected by Denaturing Gradient Gel Electrophoresis and sequencing, most of which were thermophilic anaerobes. Some bacteria could produce low molecular weight organic acids, CO_2 and H_2, which could be used as substrates by other bacteria and archaea, all of these microorganisms form an entire ecology system under physical simulation conditions.