CAJ | 학술논문

以乙二醇水溶液为溶剂,采用高沸醇溶剂法(high boiling solvent,HBS)从胡萝卜中提取木质素.结果表明,将胡萝卜干粉投入到质量分数为80%的乙二醇中,在210 ℃、料液质量比为1: 6的条件下蒸煮2 h,木质素收率最高(17.79%).采用红外光谱(FTIR)、紫外光谱(UV)和核磁共振(~1H NMR)等测试技术对其结构进行了表征.结果表明,高沸醇法提取的胡萝卜木质素具有木质素类化合物的典型结构特征.通过元素分析和甲氧基含量的测定,得到胡萝卜木质素的C_9结构模型为C_9H_(9.62)O_(1.99)(OCH_3)_(0.96).利用Fenton反应产生羟基自由基·OH,研究了胡萝卜木质素对羟基自由基的抑制效率.结果表明,胡萝卜木质素在较低的浓度(50 mg/L)下对羟基自由基就具有较强的抑制效果,抑制率最高可达64.9%.
이을이순수용액위용제,채용고비순용제법(high boiling solvent,HBS)종호라복중제취목질소.결과표명,장호라복간분투입도질량분수위80%적을이순중,재210 ℃、료액질량비위1: 6적조건하증자2 h,목질소수솔최고(17.79%).채용홍외광보(FTIR)、자외광보(UV)화핵자공진(~1H NMR)등측시기술대기결구진행료표정.결과표명,고비순법제취적호라복목질소구유목질소류화합물적전형결구특정.통과원소분석화갑양기함량적측정,득도호라복목질소적C_9결구모형위C_9H_(9.62)O_(1.99)(OCH_3)_(0.96).이용Fenton반응산생간기자유기·OH,연구료호라복목질소대간기자유기적억제효솔.결과표명,호라복목질소재교저적농도(50 mg/L)하대간기자유기취구유교강적억제효과,억제솔최고가체64.9%.
Lignin was extracted from carrot root with high boiling solvent(HBS) 80% ethylene glycol aqueous solution as solvent. Results show that the optimum conditions were as follows:the mass ratio of raw solid materials to solvent was 1: 6, temperature was 210 ℃, reaction time was 2 h, the maximum yield was 17.79%. The chemical structure of the carrot lignin has been characterized by FTIR, UV, and ~1H NMR. The chemical composition of HBS lignin from carrot root was determined by elemental analysis, and the content of methoxyl was tested with Vieb(o)ck's methods. The C_9 structure of lignin from carrot was estimated as C_9H_(9.62)O_(1.99)(OCH_3)_(0.96). The elimination efficiency of ·OH free radical by lignin from carrot was studied. The results show that carrot lignin can inhibit the activity of ·OH free radical at low concentrations（50 mg/L）with a maximum inhibition efficiency of 64.9%.