CAJ | 학술논문

目的:对不同地区千金子药材及其炮制品千金子霜中泻下成分续随二萜酯和千金二萜醇二乙酸苯甲酸酯的含量进行测定.方法:采用高效液相色谱法,流动相为甲醇-水(68:32),kromasil C_(18)柱(150 mm×4.6 mm,5 μm),柱温:40℃,检测波长:280 nm.结果:续随二萜酯和千金二萜醇二乙酸苯甲酸酯分别在0.200 μg～1.200 μg、0.206 μg～1.651 μg范围内,线性关系良好,r=0.999 7和r=0.999 9.不同产地千金子中所含两种成分的量略有差别,续随二萜酯的质量分数在0.450 8%～0.625 1%,多数在0.450 0%以上.千金二萜醇二乙酸苯甲酸酯的质量分数在0.692 3%～0.752 9%,多数在0.720 0%以上.千金子霜中两种成分的质量分数分别在0.156 8%～0.221 1%(多数在0.200 0%以下)和0.232 2%～0.283 0%(多数在0.300 0%以下).结论:建立了高效液相色谱法测定千金子脂肪油中两种泻下成分含量的方法,该方法准确、可靠,可用于毒性中药千金子及其炮制品的质量控制.
목적:대불동지구천금자약재급기포제품천금자상중사하성분속수이첩지화천금이첩순이을산분갑산지적함량진행측정.방법:채용고효액상색보법,류동상위갑순-수(68:32),kromasil C_(18)주(150 mm×4.6 mm,5 μm),주온:40℃,검측파장:280 nm.결과:속수이첩지화천금이첩순이을산분갑산지분별재0.200 μg～1.200 μg、0.206 μg～1.651 μg범위내,선성관계량호,r=0.999 7화r=0.999 9.불동산지천금자중소함량충성분적량략유차별,속수이첩지적질량분수재0.450 8%～0.625 1%,다수재0.450 0%이상.천금이첩순이을산분갑산지적질량분수재0.692 3%～0.752 9%,다수재0.720 0%이상.천금자상중량충성분적질량분수분별재0.156 8%～0.221 1%(다수재0.200 0%이하)화0.232 2%～0.283 0%(다수재0.300 0%이하).결론:건립료고효액상색보법측정천금자지방유중량충사하성분함량적방법,해방법준학、가고,가용우독성중약천금자급기포제품적질량공제.
AIM:To determine the contents of euphorbiasteroid and lathyrol diadetate benzozte in Semen Euphorbiae and Semen Euphorbiae Pulveratun from different habitats.METHODS:A kromasil C_(18) column(150 mm×4.6 mm,5 μm)was used with methanol-water(68:32)as the mobile phase and UV detection was at 280 nm.RESULTS:The linear ranges of euphorbiasteroid and lathyrol diadetate benzozte were from 0.200 μg to 1.200 μg and 0.206 μg to 1.651 μg(r = 0.999 7,r = 0.999 9).The contents of euphorbiasteroid and lathyrol diadetate benzozte in Semen Euphorbiae from different habitats ranged from 0.450 8% to 0.625 1 % and 0.692 3% to 0.752 9%.The contents of two kinds of compositions in Semen Euphorbiae Pulveratun ranged from 0.156 8% to 0.221 1% and 0.232 2% to 0.283 0%.CONCLUSION:The method for quantitating euphorbiasteroid and lathyrol diadetate benzozte in Semen Euphorbiae is accurate and reliable,and can be used to control the quality of toxicity Semen Euphorbiae and Semen Euphorbiae Pulveratun.