CAJ | 학술논문

NEAT1是一个长的非编码RNA,参与了免疫反应、paraspeckle形成以及mRNA的输出等生物学过程.本研究利用PCR-RFLP方法检测了猪(Sus scrofa)NEAT1一处Cl149G单核苷酸位点的多态性,在检测的猪品种中等位基因频率变异较大.关联分析结果表明,在长白猪群体中CC,基因型个体,0日龄和17日龄猪瘟病毒(CSFV)抗体水平,0日龄平均红细胞体积和32日龄红细胞平均血红蛋白浓度显著高于GG或CG基因型；而32日龄的血小板分布宽度、血小板平均容积和大血小板比率则显著低于GG或CG基因型.实验结果可以为猪的分子标记和辅助育种提供参考.
NEAT1시일개장적비편마RNA,삼여료면역반응、paraspeckle형성이급mRNA적수출등생물학과정.본연구이용PCR-RFLP방법검측료저(Sus scrofa)NEAT1일처Cl149G단핵감산위점적다태성,재검측적저품충중등위기인빈솔변이교대.관련분석결과표명,재장백저군체중CC,기인형개체,0일령화17일령저온병독(CSFV)항체수평,0일령평균홍세포체적화32일령홍세포평균혈홍단백농도현저고우GG혹CG기인형；이32일령적혈소판분포관도、혈소판평균용적화대혈소판비솔칙현저저우GG혹CG기인형.실험결과가이위저적분자표기화보조육충제공삼고.
Nuclear enriched abundant transcript 1 (NEAT1 ) is a long non-coding RNA that is involved in immune response,paraspeckle formation and mRNA export.In this study,a single nucleotide polymorphism C1149G in porcine (Sus scrofa) NEAT1 was detected by PCR-RFLP.Allele frequencies varied greatly among different pig breeds examined,and the association results indicated that piglets with the genotype CC had significantly higher levels of Classical swine fever virus (CSFV) antibody (0 and 17 days),mean corpuscular volume(O days) and mean corpuscular hemoglobin content (32 days),but lower platelet distribution width (32 days),mean platelet volume (32 days) and platelet larger cell ratio (32 days) than those with genotype GG or CG in a Landrace population.The results can be provided as references to molecular markers assisted breeding for pigs.