眼科研究
眼科研究
안과연구
CHINESE OPHTHALMIC RESEARCH
2010年
2期
135-139
,共5页
碱烧伤%角膜新生血管%基质细胞衍生因子%CXCR4
堿燒傷%角膜新生血管%基質細胞衍生因子%CXCR4
감소상%각막신생혈관%기질세포연생인자%CXCR4
alkali burn%corneal neovascularization%stromal cell-derived factor-1%CXCR4
目的 探讨基质细胞衍生因子-1(SDF-1)和CXCR4在小鼠角膜碱烧伤新生血管形成中的作用.方法 应用在角膜中央放置NaOH滤纸片的方法 构建C57/BL小鼠角膜碱烧伤角膜新生血管动物模型,通过免疫组织化学、RT-PCR、Western blot法检测碱烧伤后不同时间点角膜组织中SDF-1和CXCR4 mRNA及蛋白的表达情况.结果 免疫组织化学检测显示,正常小鼠角膜基质层无明显SDF-1的阳性表达,CXCR4仅在角膜上皮层呈弱阳性表达.RT-PCR检测显示,与正常对照组相比,碱烧伤后各时间点SDF-1和CXCR4 mRNA表达均明显升高(P＜0.05);Western blot检测显示SDF-1和CXCR4蛋白的表达也可见相似的变化趋势(P＜0.05).SDF-1和CXCR4在角膜中的表达于第7天达高峰,第14天开始下降,但仍高于正常.结论 SDF-1/CXCR4在碱烧伤后小鼠角膜组织中的表达增加,在角膜碱烧伤的炎症和新生血管的形成和发展过程中可能发挥重要作用.
目的 探討基質細胞衍生因子-1(SDF-1)和CXCR4在小鼠角膜堿燒傷新生血管形成中的作用.方法 應用在角膜中央放置NaOH濾紙片的方法 構建C57/BL小鼠角膜堿燒傷角膜新生血管動物模型,通過免疫組織化學、RT-PCR、Western blot法檢測堿燒傷後不同時間點角膜組織中SDF-1和CXCR4 mRNA及蛋白的錶達情況.結果 免疫組織化學檢測顯示,正常小鼠角膜基質層無明顯SDF-1的暘性錶達,CXCR4僅在角膜上皮層呈弱暘性錶達.RT-PCR檢測顯示,與正常對照組相比,堿燒傷後各時間點SDF-1和CXCR4 mRNA錶達均明顯升高(P＜0.05);Western blot檢測顯示SDF-1和CXCR4蛋白的錶達也可見相似的變化趨勢(P＜0.05).SDF-1和CXCR4在角膜中的錶達于第7天達高峰,第14天開始下降,但仍高于正常.結論 SDF-1/CXCR4在堿燒傷後小鼠角膜組織中的錶達增加,在角膜堿燒傷的炎癥和新生血管的形成和髮展過程中可能髮揮重要作用.
목적 탐토기질세포연생인자-1(SDF-1)화CXCR4재소서각막감소상신생혈관형성중적작용.방법 응용재각막중앙방치NaOH려지편적방법 구건C57/BL소서각막감소상각막신생혈관동물모형,통과면역조직화학、RT-PCR、Western blot법검측감소상후불동시간점각막조직중SDF-1화CXCR4 mRNA급단백적표체정황.결과 면역조직화학검측현시,정상소서각막기질층무명현SDF-1적양성표체,CXCR4부재각막상피층정약양성표체.RT-PCR검측현시,여정상대조조상비,감소상후각시간점SDF-1화CXCR4 mRNA표체균명현승고(P＜0.05);Western blot검측현시SDF-1화CXCR4단백적표체야가견상사적변화추세(P＜0.05).SDF-1화CXCR4재각막중적표체우제7천체고봉,제14천개시하강,단잉고우정상.결론 SDF-1/CXCR4재감소상후소서각막조직중적표체증가,재각막감소상적염증화신생혈관적형성화발전과정중가능발휘중요작용.
Background Recent research showed that stromal cell-derived factor-1 (SDF-1) plays critical role in mediating inflammation,metastasis of tumour and neovascularization of tumour.However,There is still no report about the research of the effects of SDF-1 on alkali-burn-induced corneal neovascularization (CNV).Objective The aim of this study attempts to investigate the role of SDF-1 and chemokine receptor CXCR4 in alkali-induced corneal neovascularization in mice.Methods Alkali-induced-corneal neovascularization animal models were constructed of in 15 eyes of 15 clean C57/BL mice by placing the filter paper with 1 mol/L NaOH to the central cornea for 10 seconds.The animals were sacrificed and specimens of cornea were obtained in 3,7,14 days after alkali burn of cornea.The expression of SDF-1/CXCR4 protein in the corneal tissues was detected by immunohistochemistry and Western blot,and expression of SDF-1/CXCR4 mRNA was detected by reverse transcription PCR (RT-PCR).Results SDF-1 was absent expressed and CXCR4 was faintly stained in normal cornea.The expression level of SDF-1 and CXCR4 was significantly enhanced in different time points after alkali burn in comparison with normal control mice by RT-PCR(P＜0.05),and the same trend was seen in the expression level of SDF-1 and CXCR4 proteins by Western blot (P＜0.05).The expression level of SDF-1 and CXCR4 in cornea peaked in the seventh day and began to decline in the fourteenth day but was still higher than normal level.Conclusion The study indicates that SDF-1/CXCR4 plays an important role in the formation of corneal new vessel in alkali-burn-induced animal model.
