中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2010年
1期
47-51
,共5页
黄振校%李文峰%林森%黄亚%杜季梅%谭映霞%方潇碧%张春鸿%方渭清%廖志苏
黃振校%李文峰%林森%黃亞%杜季梅%譚映霞%方瀟碧%張春鴻%方渭清%廖誌囌
황진교%리문봉%림삼%황아%두계매%담영하%방소벽%장춘홍%방위청%료지소
鼻咽肿瘤%肿瘤复发%局部%寡核苷酸序列分析%基因表达%染色体定位
鼻嚥腫瘤%腫瘤複髮%跼部%寡覈苷痠序列分析%基因錶達%染色體定位
비인종류%종류복발%국부%과핵감산서렬분석%기인표체%염색체정위
Nasopharyngeal neoplasmas%Neoplasm recurrence,local%Oligonucleotide array sequence analysis%Gene expression%Chromosome positioning
目的 通过基因芯片和生物信息学来筛选复发鼻咽癌(recurrent nasopharyngeal carcinoma,rNPC)差异基因及染色体定位和功能分析.方法 选择初发鼻咽癌(primary nasopharyngeal carcinoma,pNPC)和rNPC,应用Affymetrix Gene1.0 ST芯片筛选差异基因;应用生物信息学对差异基因进行染色体定位和功能分析.结果 差异基因共44个,其中下调基因36个,上调基因8个.基因功能分类显示,下调基因大部分属于酶活性基因(10个,27.8%),其次为钙黏合基因(7个,19.4%)、蛋白结合基因(5个,13.9%),这3类基因占了下调基因总数的61.1%,另外,受体活性基因(4个,11.1%),转录因子基因和三磷酸腺苷黏合基因各2个(各占5.6%),细胞外基质黏合基因和生长因子黏合基因各1个(各占2.8%),4个基因的功能未知(11.1%);上调基因中,3个基因的功能未知(37.5%),酶活性基因(2个,25.0%),受体活性、钙黏合、电压门压通道调控基因各1个,各占12.5%.这些差异基因分布在大多数染色体上,主要分布在1和17号染色体上.1号染色体上包含10个基因(22.7%),17号染色体包含5个基因(11.3%).结论 差异基因分布在大多数染色体上,但主要分布在1和17号染色体上,酶活性、钙黏合及蛋白结合基因这三类差异基因可能在rNPC中扮演着重要的角色,需要进一步深入研究.
目的 通過基因芯片和生物信息學來篩選複髮鼻嚥癌(recurrent nasopharyngeal carcinoma,rNPC)差異基因及染色體定位和功能分析.方法 選擇初髮鼻嚥癌(primary nasopharyngeal carcinoma,pNPC)和rNPC,應用Affymetrix Gene1.0 ST芯片篩選差異基因;應用生物信息學對差異基因進行染色體定位和功能分析.結果 差異基因共44箇,其中下調基因36箇,上調基因8箇.基因功能分類顯示,下調基因大部分屬于酶活性基因(10箇,27.8%),其次為鈣黏閤基因(7箇,19.4%)、蛋白結閤基因(5箇,13.9%),這3類基因佔瞭下調基因總數的61.1%,另外,受體活性基因(4箇,11.1%),轉錄因子基因和三燐痠腺苷黏閤基因各2箇(各佔5.6%),細胞外基質黏閤基因和生長因子黏閤基因各1箇(各佔2.8%),4箇基因的功能未知(11.1%);上調基因中,3箇基因的功能未知(37.5%),酶活性基因(2箇,25.0%),受體活性、鈣黏閤、電壓門壓通道調控基因各1箇,各佔12.5%.這些差異基因分佈在大多數染色體上,主要分佈在1和17號染色體上.1號染色體上包含10箇基因(22.7%),17號染色體包含5箇基因(11.3%).結論 差異基因分佈在大多數染色體上,但主要分佈在1和17號染色體上,酶活性、鈣黏閤及蛋白結閤基因這三類差異基因可能在rNPC中扮縯著重要的角色,需要進一步深入研究.
목적 통과기인심편화생물신식학래사선복발비인암(recurrent nasopharyngeal carcinoma,rNPC)차이기인급염색체정위화공능분석.방법 선택초발비인암(primary nasopharyngeal carcinoma,pNPC)화rNPC,응용Affymetrix Gene1.0 ST심편사선차이기인;응용생물신식학대차이기인진행염색체정위화공능분석.결과 차이기인공44개,기중하조기인36개,상조기인8개.기인공능분류현시,하조기인대부분속우매활성기인(10개,27.8%),기차위개점합기인(7개,19.4%)、단백결합기인(5개,13.9%),저3류기인점료하조기인총수적61.1%,령외,수체활성기인(4개,11.1%),전록인자기인화삼린산선감점합기인각2개(각점5.6%),세포외기질점합기인화생장인자점합기인각1개(각점2.8%),4개기인적공능미지(11.1%);상조기인중,3개기인적공능미지(37.5%),매활성기인(2개,25.0%),수체활성、개점합、전압문압통도조공기인각1개,각점12.5%.저사차이기인분포재대다수염색체상,주요분포재1화17호염색체상.1호염색체상포함10개기인(22.7%),17호염색체포함5개기인(11.3%).결론 차이기인분포재대다수염색체상,단주요분포재1화17호염색체상,매활성、개점합급단백결합기인저삼류차이기인가능재rNPC중분연착중요적각색,수요진일보심입연구.
Objective To indentify differentially expressed genes in recurrent nasopharyngeal carcinoma(rNPC) by DNA microarrays,and analyze chromosomal lecalizations and molecular function by bioinformatics. Methods The primary nasopharyngeal carcinoma (pNPC) tissue samples and rNPC tissue samples were selected, and Affymetrix Gene1.0 ST gene chips were used to indentify differential expressed genes in rNPC, and the bioinformatics was used to analyze their chromosomal localizations as well as molecular functions. Results A total of 44 genes were identified to be differential expressed in rNPC.Thirty-six genes were down regulated, 8 genes were up regulated. Functional classification of down-regulation genes showed that most genes (10 genes,27.8%) belonged to the enzyme activity genes, followed by calcium ion binding genes (7 genes, 19.4% ), protein binding genes (5 genes, 13.9% ), receptor activity genes (4 genes, 11.1% ), ATP binding genes (2 genes, 5.6%), transcription factor genes(2 genes,5.6%), extracellular matrix binding and growth factor binding have 1 gene respectively (each accounted for 2.8%). In addition, the functions of 4 genes (11.1%) were unknown.Functional classification of up-regulation genes showed most genes (3 genes, 37.5%) were unknown, followed enzyme activity genes (2 genes, 25.0%) ,receptor activity, calcium ion binding and voltage-gated ion channel activity genes have 1 genes respectively (each accounted for 12.5% ). These genes were localized randomly on the most the chromosomes, with a majority of them localized on chromosomes 1,17. Chromosome 1 contained the most differentially expressed genes (10, 22.7% ), followed by chromosomes 17(5,11.3%). Conclusions The differential expressed genes in rNPC were supposed to be randomly distributed on most chromosomes, but the majorities were found on chromosomes 1, 17. Abnormality in three groups of genes, including in enzyme activity, calcium ion binding and protein binding associate genes, might play important roles in rNPC. Those genes need to be further studied.
