中国医药
中國醫藥
중국의약
CHINA MEDICINE
2012年
6期
660-661
,共2页
陈江%黄震浩%张琳琳%李皓雲%段俊丽
陳江%黃震浩%張琳琳%李皓雲%段俊麗
진강%황진호%장림림%리호운%단준려
新生血管化,生理性%老年%缺血%人抗原R%血管内皮生长因子类
新生血管化,生理性%老年%缺血%人抗原R%血管內皮生長因子類
신생혈관화,생이성%노년%결혈%인항원R%혈관내피생장인자류
Neovascularization,physiologic%Old age%Ischemic%Human antigen R%Vascular endothelial growth factors
目的 探讨年龄对大鼠后肢缺血后血管新生的影响及与人抗原R(HuR)的关系.方法 按鼠龄将雄性SD大鼠分为2组:老龄组(18月龄)和青年组(16周龄),每组6只.建立后肢缺血模型,于术后第14天取缺血后的后肢内收肌组织,免疫组织化学法检测CD31在缺血肌肉组织中表达及新生血管数量,用蛋白印迹法检测HuR蛋白表达及用酶联免疫吸附测定法测定血管内皮生长因子(VEGF)含量.结果 老龄组缺血肌肉组织中毛细血管计数明显低于青年组[(64±7)个/mm2比(144±21)个/mm2,P＜0.05],HuR 蛋白表达明显低于青年组[(1.1±0.1)比(1.5±0.4),P＜0.05],VEGF含量也明显低于青年组[(260±24) μg/L比(351±78) μg/L,P＜0.05].结论 年龄对缺血后血管新生有明显影响,年龄越大缺血后血管新生抑制作用越强,这一作用可能与年龄增加后HuR蛋白表达下降,导致VEGF表达下降有关.
目的 探討年齡對大鼠後肢缺血後血管新生的影響及與人抗原R(HuR)的關繫.方法 按鼠齡將雄性SD大鼠分為2組:老齡組(18月齡)和青年組(16週齡),每組6隻.建立後肢缺血模型,于術後第14天取缺血後的後肢內收肌組織,免疫組織化學法檢測CD31在缺血肌肉組織中錶達及新生血管數量,用蛋白印跡法檢測HuR蛋白錶達及用酶聯免疫吸附測定法測定血管內皮生長因子(VEGF)含量.結果 老齡組缺血肌肉組織中毛細血管計數明顯低于青年組[(64±7)箇/mm2比(144±21)箇/mm2,P＜0.05],HuR 蛋白錶達明顯低于青年組[(1.1±0.1)比(1.5±0.4),P＜0.05],VEGF含量也明顯低于青年組[(260±24) μg/L比(351±78) μg/L,P＜0.05].結論 年齡對缺血後血管新生有明顯影響,年齡越大缺血後血管新生抑製作用越彊,這一作用可能與年齡增加後HuR蛋白錶達下降,導緻VEGF錶達下降有關.
목적 탐토년령대대서후지결혈후혈관신생적영향급여인항원R(HuR)적관계.방법 안서령장웅성SD대서분위2조:노령조(18월령)화청년조(16주령),매조6지.건립후지결혈모형,우술후제14천취결혈후적후지내수기조직,면역조직화학법검측CD31재결혈기육조직중표체급신생혈관수량,용단백인적법검측HuR단백표체급용매련면역흡부측정법측정혈관내피생장인자(VEGF)함량.결과 노령조결혈기육조직중모세혈관계수명현저우청년조[(64±7)개/mm2비(144±21)개/mm2,P＜0.05],HuR 단백표체명현저우청년조[(1.1±0.1)비(1.5±0.4),P＜0.05],VEGF함량야명현저우청년조[(260±24) μg/L비(351±78) μg/L,P＜0.05].결론 년령대결혈후혈관신생유명현영향,년령월대결혈후혈관신생억제작용월강,저일작용가능여년령증가후HuR단백표체하강,도치VEGF표체하강유관.
Objective To observe the effects of age on angiogenesis in ischemic hindlimb of rat and its relationship with human antigen R.Methods Male SD rats were divided into two groups:the elderly group (18 months) and young group (16 weeks).Unilateral hindlimb ischemia was deliberately manipulated by excising the entire femoral art and vein in left femoral of animals.After 14 days of surgery,the ischemic and adductor muscle tissues from hindlimb were initiated with immunostain by the endothelial marker CD31.The western blot was used to detect the expression of human antigen R (HuR) protein; vascular endothelial growth factor (VEGF) levels was detected by enzyme-linked immunosorbent assay.Results The capillary counts showed that the angiogenesis situation of elderly group was significantly worse than that of the young group ( P ＜ 0.05 ).The HuR protein expression in elderly group was significantly lower than that in the young group ( P ＜ 0.05 ) ; VEGF levels in elderly group decreased significantly,compared with the young group (P ＜0.05).Conclusion The inhibition effect on angiogenesis may increase when one is getting old,which may be related to the decreasing of HuR protein and a decline in VEGF expression.
