CAJ | 학술논문

"我院于1992午研制成长征-1多器官保存液(简称CZ-1液).同时对心肌组织0、18、24、36、48小时冷冻保存后的形态学作了观察,并与UW液作了比较.结果表明:(1)从光镜观察来看,24小时后心肌细胞浊肿,部分横纹不清.肌纤维紊乱,有断裂.肌纤维问隙增宽.48小时后心肌细胞散在性坏死.其CZ-1液组与UW液组无明显区别.(2)从电镜观察来看,18小时后心肌细胞受到轻一中度损伤.线粒体肿胀,部分嵴膜消失,出现""气球样"".24小时后心肌细胞受到严重的损伤.而UW液保存组其心肌组织保存18小时以后电镜下超微结构的损伤似乎重于CZ-1液.我们认为,自制的CZ-1液能较为有效地保存兔心脏存18小时以内."
"아원우1992오연제성장정-1다기관보존액(간칭CZ-1액).동시대심기조직0、18、24、36、48소시냉동보존후적형태학작료관찰,병여UW액작료비교.결과표명:(1)종광경관찰래간,24소시후심기세포탁종,부분횡문불청.기섬유문란,유단렬.기섬유문극증관.48소시후심기세포산재성배사.기CZ-1액조여UW액조무명현구별.(2)종전경관찰래간,18소시후심기세포수도경일중도손상.선립체종창,부분척막소실,출현""기구양"".24소시후심기세포수도엄중적손상.이UW액보존조기심기조직보존18소시이후전경하초미결구적손상사호중우CZ-1액.아문인위,자제적CZ-1액능교위유효지보존토심장존18소시이내."
Rabbit heart tissues cold stored with CZ-1 solution were histologically investigated and were compared with those stored with UW solution.Under light microscope,24h after cold storage,the cardiac muscle cells were found to have cloudy swelling with nluscle fiber disarranged or broken.The spacing between fibers were widened.48h after storage,cardiac muscle cells showed scattered necrosis.No significant histological difference was found between CZ-1 and UW groups.Under electron microscope,18h after storage,the cardiac muscle cells revealed mild to moderate injury.The mitochondria were swelling with the ballooning degeneration or crest disappearing.24h later,the myofibrils were broken and lysed;the mitochondria became swelling and ruptured with liquefaetive degeneration and pyknosis.The morphological changes in CZ-1 solution group were slighter to UW solution group within 18h of cold storage.This showed that within 8h of cold storage,CZ-1 solution is effective in the preservation of rabbit heart.