CAJ | 학술논문

目的观察经静脉移植骨髓基质细胞(BMSCs)及血管内皮祖细胞(EPCs)后血管内皮生长因子(VEGF)、基质细胞衍生因子-1(SDF-1)、碱性成纤维生长因子(bFGF)、胰岛素样生长因子-1(IGF-1)、转化生长因子β(TGF-β)、血小板源性生长因子(PDGF)、脑源性生长因子(BDNF)、胶质源性生长因子(GDNF)和神经生长因子(NGF)这9种细胞因子的表达. 方法 80只健康成年Wistar 大鼠按照随机数字表法分为模型对照组、BMSCs移植组、EPCs移植组及联合移植组,每组20只.线栓法建立大鼠大脑中动脉闭塞模型.造模24h后,分别取浓度为3x106个/mL的BMSCs、EPCs悬液及两者混合液1mL经鼠尾静脉移植入BMSCs移植组、EPCs移植组及联合移植组;模型对照组鼠尾静脉注射1 mL生理盐水.移植后7d,实时荧光PCR、Western boltting检测各组大鼠脑内生长因子的表达. 结果联合移植组中bFGF、VEGF、BDNF mRNA表达水平最高,与其他3组比较差异均有统计学意义(P＜0.05).BMSCs移植组中NGF、GDNF、TGF-β mRNA表达水平最高,与其他3组比较差异均有统计学意义(P＜0.05),联合移植组次之.EPCs组中PDGF-BB、IGF-1、SDF-1 mRNA表达水平最高,与其他3组比较差异均有统计学意义(P＜0.05),联合移植组次之. 结论 BMSCs及EPCs联合静脉移植可上调脑内生长因子表达水平,为提高细胞移植效率提供了新的尝试途径.
목적 관찰경정맥이식골수기질세포(BMSCs)급혈관내피조세포(EPCs)후혈관내피생장인자(VEGF)、기질세포연생인자-1(SDF-1)、감성성섬유생장인자(bFGF)、이도소양생장인자-1(IGF-1)、전화생장인자β(TGF-β)、혈소판원성생장인자(PDGF)、뇌원성생장인자(BDNF)、효질원성생장인자(GDNF)화신경생장인자(NGF)저9충세포인자적표체. 방법 80지건강성년Wistar 대서안조수궤수자표법분위모형대조조、BMSCs이식조、EPCs이식조급연합이식조,매조20지.선전법건립대서대뇌중동맥폐새모형.조모24h후,분별취농도위3x106개/mL적BMSCs、EPCs현액급량자혼합액1mL경서미정맥이식입BMSCs이식조、EPCs이식조급연합이식조;모형대조조서미정맥주사1 mL생리염수.이식후7d,실시형광PCR、Western boltting검측각조대서뇌내생장인자적표체. 결과 연합이식조중bFGF、VEGF、BDNF mRNA표체수평최고,여기타3조비교차이균유통계학의의(P＜0.05).BMSCs이식조중NGF、GDNF、TGF-β mRNA표체수평최고,여기타3조비교차이균유통계학의의(P＜0.05),연합이식조차지.EPCs조중PDGF-BB、IGF-1、SDF-1 mRNA표체수평최고,여기타3조비교차이균유통계학의의(P＜0.05),연합이식조차지. 결론 BMSCs급EPCs연합정맥이식가상조뇌내생장인자표체수평,위제고세포이식효솔제공료신적상시도경.
Objective To observe the expression of growth factors (vascular endothelial growth factor [VEGF],stromal cell-derived factor-1 [SDF-1 ],basic fibroblast growth factor [bFGF],insulin-like growth factor [IGF-1],transforming growth factor-β [TGF-β],platelet-derived growth factor [PDGF],brain derived neurotrophic factor [BDNF],glial cell line-derived neurotrophic factor [GDNF] and nerve growth factor [NGF]) in rat ischemic brain tissues after intravenous implantation of bone marrow stromal cells (BMSCs) and/or endothelial progenitor cells (EPCs). Methods Healthy adult Wistar rats were randomly divided into 4 groups:vehicle group,BMSCs transplantation group,EPCs transplantation group and BMSCs combined with EPCs transplantation group (n=20). The rats were subjected to middle cerebral artery occlusion (MCAO),and 24 h after that,they were intravenously transplanted with either 3×106 BMSCs,EPCs,BMSCs/EPCs or 1 mL physiological saline.Seven d after transplantation,real time-PCR and Western blotting were employed to detect the expressions of VEGF,SDF-1,bFGF,IGF-1,TGF-β,PDGF-BB,BDNF,GDNF and NGF. Results The mRNA expressions of bFGF,VEGF and BNDF in the BMSCs/EPCs transplantation group were significantly higher as compared with those in the other groups (P＜0.05).BMSCs transplantation group enjoyed the highest mRNA levels of NGF,GDNF and TGF-β among all the groups, significantly higher as compared with those in the other groups (P＜0.05),followed by BMSCs/EPCs transplantation group.EPCs transplantation group enjoyed the highest mRNA levels of PDGF,IGF-1 and SDF-1,significantly higher as compared with those in the other groups (P＜ 0.05), followed by BMSCs/EPCs transplantation group. Conclusion BMSCs combined with EPCs implantation can promote the functional rehabilitation in rats after focal cerebral ischemia, which provides new way for improving the transplantation success rate.