CAJ | 학술논문

目的构建含活性真皮基质的组织工程皮肤. 方法将人成纤维细胞(Fb)与Ⅰ型牛胶原混合接种于猪脱细胞真皮基质(PADM)的表面,构建活性真皮替代物.其上接种人表皮细胞进行气-液面培养,获得组织工程皮肤,进行组织学观察. 结果 Fb在胶原内结构完整,与PADM形成复合真皮基质.所构建的组织工程皮肤表皮层结构与人正常皮肤相似,具备基底层、棘层、颗粒层和角质层,细胞之间有桥粒连接,细胞分化良好. 结论 Fb-胶原-PADM真皮替代物可作为较好的构建组织工程皮肤的真皮支架.
목적 구건함활성진피기질적조직공정피부. 방법장인성섬유세포(Fb)여Ⅰ형우효원혼합접충우저탈세포진피기질(PADM)적표면,구건활성진피체대물.기상접충인표피세포진행기-액면배양,획득조직공정피부,진행조직학관찰. 결과 Fb재효원내결구완정,여PADM형성복합진피기질.소구건적조직공정피부표피층결구여인정상피부상사,구비기저층、극층、과립층화각질층,세포지간유교립련접,세포분화량호. 결론 Fb-효원-PADM진피체대물가작위교호적구건조직공정피부적진피지가.
Objective To construct of tissue engineering skin including active composite dermal ma- trix. Methods The human fibroblasts and bovine collagen with type Ⅰ were inoculated on the surface of porcine acellular dermal matrix ( PADM ) for construction of active dermal substitute, then epidermal cells were inoculated on the dermal matrix for gas-liquid interface culture. The tissue-engineering skin was ob- served by histological examinations. Results The structure of fibroblasts in collagen was intact,which was used to construct composite dermal matrix with PADM . The epithelial structure of tissue-engineering skin was similar to that of normal skin with good cell differentiation. Some phenomena were showed in epidermis: basic layer, stratum spinosum , granular layer and stratum corneum, desmosomes. Conclusion Fibroblasts-Col- lagen-PADM can be an optimal dermal matrix for construction of tissue-engineering skin.