中华肾脏病杂志
中華腎髒病雜誌
중화신장병잡지
2009年
3期
191-197
,共7页
陈丹%易著文%刘喜红%何庆南%黄丹琳%吴小川%莫双红
陳丹%易著文%劉喜紅%何慶南%黃丹琳%吳小川%莫雙紅
진단%역저문%류희홍%하경남%황단림%오소천%막쌍홍
肾小管坏死,急性%细胞增殖%细胞凋亡%间质干细胞移植
腎小管壞死,急性%細胞增殖%細胞凋亡%間質榦細胞移植
신소관배사,급성%세포증식%세포조망%간질간세포이식
Kidney tubular necrosis,acute%Cell proliferation%Apoptosis%Mesenchymal stem cell transplantation
目的 将胚胎后肾间充质细胞移植到大鼠右肾包膜下,探讨移植细胞对宿主急性肾小管坏死(ATN)的保护作用.方法 体质量相近的SD大鼠按随机数宁表法分为正常对照(n=24)、ATN(n=35)、ATN加假手术(n=37)、ATN加培养基(n=36)、ATN加细胞移植(n=29)共5组.首剂庆大霉素注射后第5、8、11、14天每组分别取6只大鼠处死,留取血和肾组织标本.4,6-联脒-2-苯基吲哚(DAPI)示踪法观察移植细胞的分布.全自动生化分析仪检测Scr和尿NAG酶水平.HE染色法进行肾组织病理评分.免疫组化染色检测肾皮质Ki-67和Bcl-2阳性表达细胞,分别计算增殖指数和Bcl-2蛋白的相对表达量.TUNEL法检测肾皮质细胞的凋亡指数.结果 胚胎后肾间充质细胞被扩增,波形蛋白染色阳性,角蛋白染色阴性,符合未分化的间充质细胞的特点.与ATN组、ATN加假手术、ATN加培养基组大鼠比较,ATN加细胞移植组大鼠Scr和尿NAG酶较低[第14天Scr:(101.38±20.46)μmol/L比(248.78±23.15)、(252.98±33.52)、(229.08±18.18)μmol/L;NAG酶:(14.83±7.74)U/L比(33.33±14.88)、(29.62±10.54)、(30.22±10.94)U/L,均P<0.05];右肾皮质病理积分降低(P<0.05);肾小管上皮细胞增殖增加(P<0.05),凋亡减少(P<0.05);Bcl-2蛋白表达水平上调(P<0.05).结论 肾包膜下移植胚胎后肾问充质细胞能够修复损伤的肾脏,改善肾功能.
目的 將胚胎後腎間充質細胞移植到大鼠右腎包膜下,探討移植細胞對宿主急性腎小管壞死(ATN)的保護作用.方法 體質量相近的SD大鼠按隨機數寧錶法分為正常對照(n=24)、ATN(n=35)、ATN加假手術(n=37)、ATN加培養基(n=36)、ATN加細胞移植(n=29)共5組.首劑慶大黴素註射後第5、8、11、14天每組分彆取6隻大鼠處死,留取血和腎組織標本.4,6-聯脒-2-苯基吲哚(DAPI)示蹤法觀察移植細胞的分佈.全自動生化分析儀檢測Scr和尿NAG酶水平.HE染色法進行腎組織病理評分.免疫組化染色檢測腎皮質Ki-67和Bcl-2暘性錶達細胞,分彆計算增殖指數和Bcl-2蛋白的相對錶達量.TUNEL法檢測腎皮質細胞的凋亡指數.結果 胚胎後腎間充質細胞被擴增,波形蛋白染色暘性,角蛋白染色陰性,符閤未分化的間充質細胞的特點.與ATN組、ATN加假手術、ATN加培養基組大鼠比較,ATN加細胞移植組大鼠Scr和尿NAG酶較低[第14天Scr:(101.38±20.46)μmol/L比(248.78±23.15)、(252.98±33.52)、(229.08±18.18)μmol/L;NAG酶:(14.83±7.74)U/L比(33.33±14.88)、(29.62±10.54)、(30.22±10.94)U/L,均P<0.05];右腎皮質病理積分降低(P<0.05);腎小管上皮細胞增殖增加(P<0.05),凋亡減少(P<0.05);Bcl-2蛋白錶達水平上調(P<0.05).結論 腎包膜下移植胚胎後腎問充質細胞能夠脩複損傷的腎髒,改善腎功能.
목적 장배태후신간충질세포이식도대서우신포막하,탐토이식세포대숙주급성신소관배사(ATN)적보호작용.방법 체질량상근적SD대서안수궤수저표법분위정상대조(n=24)、ATN(n=35)、ATN가가수술(n=37)、ATN가배양기(n=36)、ATN가세포이식(n=29)공5조.수제경대매소주사후제5、8、11、14천매조분별취6지대서처사,류취혈화신조직표본.4,6-련미-2-분기신타(DAPI)시종법관찰이식세포적분포.전자동생화분석의검측Scr화뇨NAG매수평.HE염색법진행신조직병리평분.면역조화염색검측신피질Ki-67화Bcl-2양성표체세포,분별계산증식지수화Bcl-2단백적상대표체량.TUNEL법검측신피질세포적조망지수.결과 배태후신간충질세포피확증,파형단백염색양성,각단백염색음성,부합미분화적간충질세포적특점.여ATN조、ATN가가수술、ATN가배양기조대서비교,ATN가세포이식조대서Scr화뇨NAG매교저[제14천Scr:(101.38±20.46)μmol/L비(248.78±23.15)、(252.98±33.52)、(229.08±18.18)μmol/L;NAG매:(14.83±7.74)U/L비(33.33±14.88)、(29.62±10.54)、(30.22±10.94)U/L,균P<0.05];우신피질병리적분강저(P<0.05);신소관상피세포증식증가(P<0.05),조망감소(P<0.05);Bcl-2단백표체수평상조(P<0.05).결론 신포막하이식배태후신문충질세포능구수복손상적신장,개선신공능.
Objective To evaluate the nephroprotective effects of transplanting metanephric mesenchymal cells (MMCs) into the renal subcaspsule of rats with acute tubular necrosis (ATN) induced by gentamicin. Methods MMCs were expanded in culture and immunocytochemistry was used to characterize the cells. After gentamicin-induced ATN, fluorescence-labeled cells were transplanted and traced in kidney tissues by fluorescence microscopy. Serum creatinine (Scr) and N-acetyl-b-D-glucosaminidase (NAG) were tested. Kidney pathology was studied by hematoxylin-eosin staining. Apoptosis was examined by the TUNEL assay. Ki-67 and Bcl-2 expression was examined by immunohistochemistry. Results MMCs were expanded in culture and the phenotype of the cells was vimentin-positive and keratin-negative. Compared with other ATN groups, in the MMCs-treated group, Scr and NAG clearly decreased[14d Scr: (101.38±20.46) μmol/L vs (248.78±23.15), (252.98±33.52), (229.08±18.18) μmol/L;NAG: (14.83±7.74) U/L vs (33.33±14.88), (29.62±10.54), (30.22±10.94) U/L, P<0.05, respectively];the histopathoiogic lesion scores were lower (P<0.05);the Ki-67 antibody and apoptosis of renal tubular epithelial cells were improved or reduced respectively;the expression of Bcl-2 protein was up-regulated (P<0.05). Conclusion The subcapsular transplantation of MMCs can ameliorate renal function and repair kidney injury.
