CAJ | 학술논문

目的观察重组腺病毒载体介导色素上皮衍生因子(PEDF)基因对视网膜新生血管(RNV)发生的影响.方法将20只鼠龄7 d的Spraque-Dawley大鼠建立模型后随机分为2组,分别于出生后14 d行玻璃体内注射空白腺病毒载体(AV-Blank组)及编码PEDF基因的重组腺病毒载体(AV-PEDF组),采用RNV内皮细胞计数、运用逆转录聚合酶链反应(RT-PCR)和免疫组织化学法检测玻璃体、视网膜中PEDF基因及其蛋白表达的变化.结果注射药物后AV-PEDF组视网膜的新生血管数较AV-Blank组明显减少(t=42.009.P＜0.001);视网膜PEDF蛋白的表达较AV-Blank组明显增加(t=36.638,P＜0.001);玻璃体组织中的PEDF mRNA表达量也较AV-Blank组明显增加(t=9.128,P＜0.001).结论重组腺病毒载体介导的PEDF基因可上调大鼠RNV眼玻璃体及视网膜组织中PEDF的表达;推测PEDF的表达可能与RNV的抑制与消退相关.
목적 관찰중조선병독재체개도색소상피연생인자(PEDF)기인대시망막신생혈관(RNV)발생적영향.방법 장20지서령7 d적Spraque-Dawley대서건립모형후수궤분위2조,분별우출생후14 d행파리체내주사공백선병독재체(AV-Blank조)급편마PEDF기인적중조선병독재체(AV-PEDF조),채용RNV내피세포계수、운용역전록취합매련반응(RT-PCR)화면역조직화학법검측파리체、시망막중PEDF기인급기단백표체적변화.결과 주사약물후AV-PEDF조시망막적신생혈관수교AV-Blank조명현감소(t=42.009.P＜0.001);시망막PEDF단백적표체교AV-Blank조명현증가(t=36.638,P＜0.001);파리체조직중적PEDF mRNA표체량야교AV-Blank조명현증가(t=9.128,P＜0.001).결론 중조선병독재체개도적PEDF기인가상조대서RNV안파리체급시망막조직중PEDF적표체;추측PEDF적표체가능여RNV적억제여소퇴상관.
Objective To invesitgate the influence of recombinant adenovirus vector of humanpigment epithelium-derived factor(AV-hPEDF)on retinal new vessels mediated by recombinant adenovirusvector. Methods Twenty 7-days-old Sprague-Dawley(SD)rat were divided into two groups randomlyafter the establishment of retinal neovascularization model.At postnatal 14 day,they were acceptedintravitreal injection with blank-adenovirus-vector(AV-Blank group)and adenovirus-vector PEDF(AV-PEDF group)respectively.The retinal vascular endothelial ceils were counted,the PEDF mRNA andprotein expression in retina and vitreous were determined by reverse transcription-polymerase chainreaction(RT-PCR)and immunohistochemistry. Results After injection with medicine,the number ofRNV was decreased obviously in AV-PEDF group(t=42.009,P＜0.001);the protein expression of retinalPEDF was increased obviously in AV-PEDF group(f=36.638.P＜0.001);the PEDF mRNA expressionin vitreous was also increased obviously in AV.PEDF group(t=9.128,P＜0.001). ConclusionRecombinant Adenovirus vector mediated PEDF can raise the PEDF expression in the retinal and vitreousneovascularized tissues in rat,which suggested that the expression of PEDF may be related to inhibitionand reduction of RNV.