CAJ | 학술논문

背景:单纯的聚乙烯醇材料对细胞的黏附能力有限,用胶原蛋白对其进行表向修饰后能否改善其对细胞的黏附和增殖作用尚无公认.目的:探讨聚乙烯醇纺丝纤维编织用Ⅰ型胶原胶表面修饰后作为构建组织工程前交叉韧带支架材料的可行性.设计、时间和地点:对比观察实验,于2006-08/2007-10在暨南大学医学院第四附属医院,广州市红十字会医院,广州市创伤外科研究所完成.材料:Ⅰ型胶原蛋白胶,由广州市创伤外科研究所研制的胶原胶制剂.方法:用聚乙烯醇纺丝编织成条束状支架材料,用NIH-3T3细胞株和人前交叉韧带细胞体外培养、扩增后,分别种植到聚乙烯醇和经Ⅰ型胶原胶修饰过的聚乙烯醇纺丝纤维编织(PVA/COL)支架材料上,立体培养.主要观察指标:通过扫描电子显微镜观察NIH-3T3细胞株和人前交叉韧带细胞在聚乙烯醇与PVA/COL纺丝编织材料上生长及细胞外基质的分泌情况,对比评价编织构建的聚乙烯醇和经Ⅰ型胶原胶修饰过的聚乙烯醇纺丝纤维编织支架材料细胞相容性的优劣.在电子拉力机测试聚乙烯醇纺丝纤维编织支架材料力学性质,用SPSS 11.5软件包分析材料的力学性能.结果:NIH-3T3细胞株和人前交叉韧带细胞在聚乙烯醇和经Ⅰ型胶原胶修饰过的聚乙烯醇纺丝纤维编织支架材料表面和孔隙内黏附增殖并能分泌细胞外基质.NIH-3T3细胞株比人前交叉韧带细胞生长旺盛.NIH-3T3细胞株和人前交叉韧带细胞在经Ⅰ型胶原胶修饰过的聚乙烯醇纺丝纤维编织支架材料上细胞黏附数量明显增多,在聚乙烯醇支架材料上细胞生长形态较好.Ⅰ型胶原胶可促进NIH-3T3细胞株分泌细胞外基质但对人前交叉韧带细胞作用不明显.拉力测试该编织材料负荷-拉伸曲线与人及兔前交叉韧带的相似,柔韧性好,最大负荷、极限应力和弹性模量分别为52.61 N,14.96 MPa和202.08 MPa.结论:Ⅰ型胶原可促进NIH-3T3细胞株和人前交叉韧带细胞在聚乙烯醇纺丝纤维编织支架材料表面和孔隙内黏附、增殖,可促进NIH-3T3细胞株分泌细胞外基质,聚乙烯醇纺丝纤维编织材料具有一定的力学性能和良好的细胞相容性. 揹景:單純的聚乙烯醇材料對細胞的黏附能力有限,用膠原蛋白對其進行錶嚮脩飾後能否改善其對細胞的黏附和增殖作用尚無公認.目的:探討聚乙烯醇紡絲纖維編織用Ⅰ型膠原膠錶麵脩飾後作為構建組織工程前交扠韌帶支架材料的可行性.設計、時間和地點:對比觀察實驗,于2006-08/2007-10在暨南大學醫學院第四附屬醫院,廣州市紅十字會醫院,廣州市創傷外科研究所完成.材料:Ⅰ型膠原蛋白膠,由廣州市創傷外科研究所研製的膠原膠製劑.方法:用聚乙烯醇紡絲編織成條束狀支架材料,用NIH-3T3細胞株和人前交扠韌帶細胞體外培養、擴增後,分彆種植到聚乙烯醇和經Ⅰ型膠原膠脩飾過的聚乙烯醇紡絲纖維編織(PVA/COL)支架材料上,立體培養.主要觀察指標:通過掃描電子顯微鏡觀察NIH-3T3細胞株和人前交扠韌帶細胞在聚乙烯醇與PVA/COL紡絲編織材料上生長及細胞外基質的分泌情況,對比評價編織構建的聚乙烯醇和經Ⅰ型膠原膠脩飾過的聚乙烯醇紡絲纖維編織支架材料細胞相容性的優劣.在電子拉力機測試聚乙烯醇紡絲纖維編織支架材料力學性質,用SPSS 11.5軟件包分析材料的力學性能.結果:NIH-3T3細胞株和人前交扠韌帶細胞在聚乙烯醇和經Ⅰ型膠原膠脩飾過的聚乙烯醇紡絲纖維編織支架材料錶麵和孔隙內黏附增殖併能分泌細胞外基質.NIH-3T3細胞株比人前交扠韌帶細胞生長旺盛.NIH-3T3細胞株和人前交扠韌帶細胞在經Ⅰ型膠原膠脩飾過的聚乙烯醇紡絲纖維編織支架材料上細胞黏附數量明顯增多,在聚乙烯醇支架材料上細胞生長形態較好.Ⅰ型膠原膠可促進NIH-3T3細胞株分泌細胞外基質但對人前交扠韌帶細胞作用不明顯.拉力測試該編織材料負荷-拉伸麯線與人及兔前交扠韌帶的相似,柔韌性好,最大負荷、極限應力和彈性模量分彆為52.61 N,14.96 MPa和202.08 MPa.結論:Ⅰ型膠原可促進NIH-3T3細胞株和人前交扠韌帶細胞在聚乙烯醇紡絲纖維編織支架材料錶麵和孔隙內黏附、增殖,可促進NIH-3T3細胞株分泌細胞外基質,聚乙烯醇紡絲纖維編織材料具有一定的力學性能和良好的細胞相容性.
배경:단순적취을희순재료대세포적점부능력유한,용효원단백대기진행표향수식후능부개선기대세포적점부화증식작용상무공인.목적:탐토취을희순방사섬유편직용Ⅰ형효원효표면수식후작위구건조직공정전교차인대지가재료적가행성.설계、시간화지점:대비관찰실험,우2006-08/2007-10재기남대학의학원제사부속의원,엄주시홍십자회의원,엄주시창상외과연구소완성.재료:Ⅰ형효원단백효,유엄주시창상외과연구소연제적효원효제제.방법:용취을희순방사편직성조속상지가재료,용NIH-3T3세포주화인전교차인대세포체외배양、확증후,분별충식도취을희순화경Ⅰ형효원효수식과적취을희순방사섬유편직(PVA/COL)지가재료상,입체배양.주요관찰지표:통과소묘전자현미경관찰NIH-3T3세포주화인전교차인대세포재취을희순여PVA/COL방사편직재료상생장급세포외기질적분비정황,대비평개편직구건적취을희순화경Ⅰ형효원효수식과적취을희순방사섬유편직지가재료세포상용성적우렬.재전자랍력궤측시취을희순방사섬유편직지가재료역학성질,용SPSS 11.5연건포분석재료적역학성능.결과:NIH-3T3세포주화인전교차인대세포재취을희순화경Ⅰ형효원효수식과적취을희순방사섬유편직지가재료표면화공극내점부증식병능분비세포외기질.NIH-3T3세포주비인전교차인대세포생장왕성.NIH-3T3세포주화인전교차인대세포재경Ⅰ형효원효수식과적취을희순방사섬유편직지가재료상세포점부수량명현증다,재취을희순지가재료상세포생장형태교호.Ⅰ형효원효가촉진NIH-3T3세포주분비세포외기질단대인전교차인대세포작용불명현.랍력측시해편직재료부하-랍신곡선여인급토전교차인대적상사,유인성호,최대부하、겁한응력화탄성모량분별위52.61 N,14.96 MPa화202.08 MPa.결론:Ⅰ형효원가촉진NIH-3T3세포주화인전교차인대세포재취을희순방사섬유편직지가재료표면화공극내점부、증식,가촉진NIH-3T3세포주분비세포외기질,취을희순방사섬유편직재료구유일정적역학성능화량호적세포상용성.
BACKGROUND: Simple polyvinyl alcohol (PVA) has limited ability to cell adhesion. There are not generally accepted studies on improved effects of collagen protein modified polyvinyl alcohol on cell adhesion and proliferation.OBJECTIVE: To investigate the feasibility of PVA/type Ⅰ college (COL-Ⅰ) as anterior cruciate ligament (ACL) scaffolds in tissue engineering.DESIGN, TIME AND SETTING: The controlled observation experiment was performed at the Fourth Affiliated Hospital, Medical College. Ji'nan University, Guangzhou Red Cross Hospital, Guangzhou Institute of Trauma Surgery from August 2006 to October 2007.MATERIALS: COL-Ⅰ gel was produced by Guangzhou Institute of Trauma Surgery.METHODS: PVA filature was used to weave fascicular scaffolds. NIH-3T3 cell line and human ACL cells were in vitro incubated, amplified, and then implanted on the PVA/COL scaffolds.MAIN OUTCOME MEASURES: The growth of NIH-3T3 cell line and human ACL cells on the PVA/COL scaffolds and the secretion of extracellular matrix were observed using scanning electron microscope. Cell compatibility of PVA/COL scaffolds was assessed. Mechanics characteristic of PVA/COL scaffolds was measured by using the electric. tensile force apparatus. Mechanical property of PVA/COL scaffolds was analyzed using the SPSS 11.5 software package.RESULTS: NIH-3T3 cell line and human ACL cells on the PVA/COL scaffolds adhered, proliferated, and secreted extracellular matrix. NIH-3T3 cell line highly grew compared with human ACL cells on the PVA/COL scaffolds. The adhered number of NIH-3T3 cell line and human ACL cells was significantly increased on the PVA/COL scaffolds. NIH-3T3 cell line and human ACL cells presented well morphology on the PVA/COL scaffolds. COL-Ⅰ could promote the secretion of extracellular matrix from NIH-3T3 cells, but its effects on human ACL cells were not significant. Tensile force test showed that load-extension curve of the materials was identical to ACL of human and rabbits, and the scaffolds possessed strong flexibility. The maximal load, ultimate stress and elastic modulus were respectively 52.61 N, 14.96 MPa and 202.08 MPa.CONCLUSION: COL-Ⅰ accelerates the adhesion and proliferation of NIH-3T3 cell line and human ACL cells on the surface and in the pore of the PVA/COL scaffolds, promotes the secretion of extracellular matrix from NIH-3T3, and PVA filature material has mechanical property and good cell compatibility.