CAJ | 학술논문

采用水培实验方法研究了万寿菊体内镉积累和解毒与巯基化合物含黾的关系.万寿菊植株分别在镉浓度为0、0.1、0.5、2和8 mg/L的营养液中暴露7 d,测定了根、茎、叶中镉、非蛋白巯基(NPT)、半胱氨酸(Cys)、γ-谷氨酰半胱氨酸(γ-EC)、谷胱甘肽(GSH)和植物络合素(PCs)的含量.植物根、茎、叶中镉含量都随着镉暴露浓度的增加而增加.当溶液中镉浓度较低(0.1～2 mg/L)时,茎叶中NPT、PCs、Cys和γ-Ec含量随着镉浓度增加而增大;当镉浓度较高(8 mg/L)时,茎叶中PCs含量迅速降低,GSH含量大幅度增高.在根部,这些巯基化合物的含量几乎不受镉处理影响,且含量较低.以上研究结果表明:PCs在万寿菊镉的解毒机制中发挥一定的作用,暴露于高浓度的镉,GSH比PCs起着更为重要的解毒作用.
채용수배실험방법연구료만수국체내력적루화해독여구기화합물함민적관계.만수국식주분별재력농도위0、0.1、0.5、2화8 mg/L적영양액중폭로7 d,측정료근、경、협중력、비단백구기(NPT)、반광안산(Cys)、γ-곡안선반광안산(γ-EC)、곡광감태(GSH)화식물락합소(PCs)적함량.식물근、경、협중력함량도수착력폭로농도적증가이증가.당용액중력농도교저(0.1～2 mg/L)시,경협중NPT、PCs、Cys화γ-Ec함량수착력농도증가이증대;당력농도교고(8 mg/L)시,경협중PCs함량신속강저,GSH함량대폭도증고.재근부,저사구기화합물적함량궤호불수력처리영향,차함량교저.이상연구결과표명:PCs재만수국력적해독궤제중발휘일정적작용,폭로우고농도적력,GSH비PCs기착경위중요적해독작용.
The relationship between thiol-containing compounds accumulation and cadmium (Cd) detoxification was studied. Studies were carried out with marigold ( Tagetes erecta L. ) plants grown in the presence of 0.1,0.5, 2, 8 mg/L Cd for 7 days. Contents of cysteine (Cys), γ-glutamyl-cysteine (γ-EC ) , glutathione (GSH) , phytochelatins (PCs) and non-protein thiols ( NPT) , and Cd accumulation in the root, stem and leaf were determined. Cd contents increased in the plant with the increase of Cd exposure concentration. From 0.1 to 2 mg/L Cd, contents of NPT, PCs, Cys and γ-EC in stems and leaves significantly increased with increasing Cd doses. However, at the dose of 8 mg/L, the synthesis of PCs in the stem and leaf decreased drastically, and the GSH pool increased significantly. In the root, contents of these thiol-containing compounds were lower and less affected by Cd treatments. These results indicate that PCs play some roles in Cd detoxification, but PCs-mediated chelation are not the most effective strategy to cope with an exposure to toxic levels of this metal.