中国人兽共患病学报
中國人獸共患病學報
중국인수공환병학보
CHINESE JOURNAL OF ZOONOSES
2010年
2期
111-114
,共4页
康雁君%沈权%张文%华修国%冉雪琴%王嘉福
康雁君%瀋權%張文%華脩國%冉雪琴%王嘉福
강안군%침권%장문%화수국%염설금%왕가복
戊型肝炎病毒%作用蛋白%酵母双杂交
戊型肝炎病毒%作用蛋白%酵母雙雜交
무형간염병독%작용단백%효모쌍잡교
hepatitis E virus%interacting protein%yeast double hybridization system
目的 筛选人肝细胞文库戊肝病毒pORF3结合蛋白,为HEV ORF3蛋白的功能研究提供依据.方法 构建pSOS-baitORF3诱饵重组载体,诱饵蛋白的自激活检测以及表达验证后,筛选cDNA文库中HEV ORF3相互作用蛋白.结果 经验证pSOS-baitORF3诱饵重组载体构建成功,诱饵蛋白的自激活检测以及表达验证均符合预期结果,共筛选出八种相互作用蛋白.结论 筛选出的蛋白基本上为功能性蛋白,其中包括免疫信号通路激酶p110δ以及线粒体膜蛋白,和补体系统C3等,有进一步研究的价值.
目的 篩選人肝細胞文庫戊肝病毒pORF3結閤蛋白,為HEV ORF3蛋白的功能研究提供依據.方法 構建pSOS-baitORF3誘餌重組載體,誘餌蛋白的自激活檢測以及錶達驗證後,篩選cDNA文庫中HEV ORF3相互作用蛋白.結果 經驗證pSOS-baitORF3誘餌重組載體構建成功,誘餌蛋白的自激活檢測以及錶達驗證均符閤預期結果,共篩選齣八種相互作用蛋白.結論 篩選齣的蛋白基本上為功能性蛋白,其中包括免疫信號通路激酶p110δ以及線粒體膜蛋白,和補體繫統C3等,有進一步研究的價值.
목적 사선인간세포문고무간병독pORF3결합단백,위HEV ORF3단백적공능연구제공의거.방법 구건pSOS-baitORF3유이중조재체,유이단백적자격활검측이급표체험증후,사선cDNA문고중HEV ORF3상호작용단백.결과 경험증pSOS-baitORF3유이중조재체구건성공,유이단백적자격활검측이급표체험증균부합예기결과,공사선출팔충상호작용단백.결론 사선출적단백기본상위공능성단백,기중포괄면역신호통로격매p110δ이급선립체막단백,화보체계통C3등,유진일보연구적개치.
HEV is a positive strand RNA virus containing 3 open reading frames(ORFs), while the function of its binding protein pORF3 is poorly understood. In our study, the interactive protein of the pORF3 from human liver cDNA library was screened by the SOS recruitment system, and the construction of the pSOS bait-ORF3 plasmid was identified. The expression of the pORF3 was analyzed by Western blotting, and the detection result of the self activation of bait protein was obtained as expected. Totally, 8 putative interacting proteins had been successively screened. Some of these cellular binding proteins of pORF3, especially the important immunological pathway kinase p110δ, may advance our understanding of the role in ORF3 protein of HEV, and the possible implications of these candidate proteins for the further research are discussed.
