CAJ | 학술논문

建立抗稻瘟病基因的分子标记对培育抗稻瘟病品种有重要意义.研究利用71个广西地方菌株检测地谷对稻瘟病菌的抗性,并利用地谷中Pi-d2基因与广恢998等位基因在基因组序列上一个碱基的差异,设计出以抗病基因本身序列为引物的基因标签M-Pid2,通过分析地谷与广恢998的F_2群体的基因型与对稻瘟病抗感分离的吻合度来验证该标签的准确性,用M-Pid2扩增62份水稻种质验证该标签的特异性.结果表明,地谷能抗71个地方稻瘟病菌株中的59个菌株,抗性频率达83.1 %.用M-Pid2扩增地谷与广恢998的F_2群体的基因型与其对稻瘟病的抗感分离完全吻合;用M-Pid2扩增62份水稻种质,仅在地谷中扩增出629 bp特异条带.从而确认抗稻瘟病基因Pi-d2为地谷的主效抗稻瘟病基因,且在广西有育种利用价值;标记M-Pid2能准确用于抗稻瘟病基因Pi-d2的辅助选择.
건립항도온병기인적분자표기대배육항도온병품충유중요의의.연구이용71개엄서지방균주검측지곡대도온병균적항성,병이용지곡중Pi-d2기인여엄회998등위기인재기인조서렬상일개감기적차이,설계출이항병기인본신서렬위인물적기인표첨M-Pid2,통과분석지곡여엄회998적F_2군체적기인형여대도온병항감분리적문합도래험증해표첨적준학성,용M-Pid2확증62빈수도충질험증해표첨적특이성.결과표명,지곡능항71개지방도온병균주중적59개균주,항성빈솔체83.1 %.용M-Pid2확증지곡여엄회998적F_2군체적기인형여기대도온병적항감분리완전문합;용M-Pid2확증62빈수도충질,부재지곡중확증출629 bp특이조대.종이학인항도온병기인Pi-d2위지곡적주효항도온병기인,차재엄서유육충이용개치;표기M-Pid2능준학용우항도온병기인Pi-d2적보조선택.
The establishment of molecular marker of rice blast resistance genes was a powerful strategy in resistance to rice blast breeding. In the present study, seventy-one strains in Digu variety was used to check the regional rice blast resistance in Guangxi. A gene tagging M-Pid2 was amplified by the primers designed with the sequence of resistance gene itself, using a base difference between Pi-d2 gene in Digu variety and Guanghui 998 allele. The validity of the gene tag was tested in a F_2 generation derived from Digu(a resistant variety including Pi-d2) and Guanghui 998(a susceptible restore line) and a group of germplasm containing 62 rice germplasm. The resistance spectrum was detected by inoculating Pi-d2 gene sponsor with 71 rice blast pathogen strains collected from Guangxi. The resistance spectrum of all materials were also detected in natural disease nursery located in Cenxi county. The results showed that the resistance indicated that Pi-d2 gene tag was highly consistent with that indicated under field detection. Furthermore, PCR analysis indicated that, among the 62 rice germplasm, a specific band of 629 bp was amplified only in Digu variety. It was concluded that rice blast resistance gene Pi-d2 was the main-effect blast resistance genes of Digu rice. and the gene tag could be credibly used in marker assisted selection and germplasm differentiation.