国际生物医学工程杂志
國際生物醫學工程雜誌
국제생물의학공정잡지
INTERNATIONAL JOURNAL OF BIOMEDICAL ENGINEERING
2010年
6期
321-325,330
,共6页
1型、2型糖尿病%大鼠%Morris水迷宫%空间记忆能力%空间关联记忆能力
1型、2型糖尿病%大鼠%Morris水迷宮%空間記憶能力%空間關聯記憶能力
1형、2형당뇨병%대서%Morris수미궁%공간기억능력%공간관련기억능력
Type1 and type2 diabetes%Rats%Morris water maze%Spatial memory ability%Spatial associative memory ability
目的 研究大鼠患糖尿病后,空间记忆能力和空间关联记忆能力受到的影响.方法 将70只SD大鼠(体质量180 g±20 g)随机分成3组,对照组、1型糖尿病组和2型糖尿病组.采用STZ一次性腹腔注射和联合高脂饲料喂养的方法分别制备1型和2型糖尿病大鼠模型,检测血糖水平.在造模成功的1个月和3个月后,分别进行水迷宫实验,包括4 d的定位航行和1 d的空间探索实验.结果 在糖尿病造模成功的1个月后,大鼠没有发生明显的空间记忆能力或空间关联记忆能力障碍.造模3个月后,在定位航行实验中,2组糖尿病大鼠的逃避潜伏期都比对照组时间长,具有统计学差异(P＜0.05).实验第2天开始,对照组逃避潜伏期的下降趋势明显,而糖尿病组下降缓慢.1型和2型糖尿病组之间的逃避潜伏期没有显著差异(P＞0.05).造模3个月后,在空间探索实验中,从训练过的Ⅰ象限入水时,2个糖尿病模型组平台象限游泳时间短,第1环点数和总得分等参数低,与对照组的差异都具有显著意义(P＜0.05),2型糖尿病组的参数值比1型糖尿病组稍低.从未训练的Ⅳ象限入水时,糖尿病模型组的参数值也均低于正常对照组,具有统计学差异(P＜0.05),且1型糖尿病组的总得分比2型糖尿病组更低一些.结论 1型或2型的糖尿病大鼠会发生空间记忆能力和空间关联记忆能力的下降.本实验中2型糖尿病组的空间记忆能力比1型糖尿病组受到了更明显的影响,而1型糖尿病组的空间关联记忆能力受到的影响比2型糖尿病组更大.
目的 研究大鼠患糖尿病後,空間記憶能力和空間關聯記憶能力受到的影響.方法 將70隻SD大鼠(體質量180 g±20 g)隨機分成3組,對照組、1型糖尿病組和2型糖尿病組.採用STZ一次性腹腔註射和聯閤高脂飼料餵養的方法分彆製備1型和2型糖尿病大鼠模型,檢測血糖水平.在造模成功的1箇月和3箇月後,分彆進行水迷宮實驗,包括4 d的定位航行和1 d的空間探索實驗.結果 在糖尿病造模成功的1箇月後,大鼠沒有髮生明顯的空間記憶能力或空間關聯記憶能力障礙.造模3箇月後,在定位航行實驗中,2組糖尿病大鼠的逃避潛伏期都比對照組時間長,具有統計學差異(P＜0.05).實驗第2天開始,對照組逃避潛伏期的下降趨勢明顯,而糖尿病組下降緩慢.1型和2型糖尿病組之間的逃避潛伏期沒有顯著差異(P＞0.05).造模3箇月後,在空間探索實驗中,從訓練過的Ⅰ象限入水時,2箇糖尿病模型組平檯象限遊泳時間短,第1環點數和總得分等參數低,與對照組的差異都具有顯著意義(P＜0.05),2型糖尿病組的參數值比1型糖尿病組稍低.從未訓練的Ⅳ象限入水時,糖尿病模型組的參數值也均低于正常對照組,具有統計學差異(P＜0.05),且1型糖尿病組的總得分比2型糖尿病組更低一些.結論 1型或2型的糖尿病大鼠會髮生空間記憶能力和空間關聯記憶能力的下降.本實驗中2型糖尿病組的空間記憶能力比1型糖尿病組受到瞭更明顯的影響,而1型糖尿病組的空間關聯記憶能力受到的影響比2型糖尿病組更大.
목적 연구대서환당뇨병후,공간기억능력화공간관련기억능력수도적영향.방법 장70지SD대서(체질량180 g±20 g)수궤분성3조,대조조、1형당뇨병조화2형당뇨병조.채용STZ일차성복강주사화연합고지사료위양적방법분별제비1형화2형당뇨병대서모형,검측혈당수평.재조모성공적1개월화3개월후,분별진행수미궁실험,포괄4 d적정위항행화1 d적공간탐색실험.결과 재당뇨병조모성공적1개월후,대서몰유발생명현적공간기억능력혹공간관련기억능력장애.조모3개월후,재정위항행실험중,2조당뇨병대서적도피잠복기도비대조조시간장,구유통계학차이(P＜0.05).실험제2천개시,대조조도피잠복기적하강추세명현,이당뇨병조하강완만.1형화2형당뇨병조지간적도피잠복기몰유현저차이(P＞0.05).조모3개월후,재공간탐색실험중,종훈련과적Ⅰ상한입수시,2개당뇨병모형조평태상한유영시간단,제1배점수화총득분등삼수저,여대조조적차이도구유현저의의(P＜0.05),2형당뇨병조적삼수치비1형당뇨병조초저.종미훈련적Ⅳ상한입수시,당뇨병모형조적삼수치야균저우정상대조조,구유통계학차이(P＜0.05),차1형당뇨병조적총득분비2형당뇨병조경저일사.결론 1형혹2형적당뇨병대서회발생공간기억능력화공간관련기억능력적하강.본실험중2형당뇨병조적공간기억능력비1형당뇨병조수도료경명현적영향,이1형당뇨병조적공간관련기억능력수도적영향비2형당뇨병조경대.
Objective To study the effect of diabetes on spatial memory ability and spatial associative memory ability in rats. Methods 70 SD rats( 180±20 g) were randomly divided into 3 groups: control group,type1 diabetes group and type2 diabetes group. Type1 and type2 diabetic rat models were set up by streptozocin (STZ) intraperitoneal injection and high fat forage raise. The blood glucose was determined. After rat diabetic model established 1 month and 3 months, respectively, the Morris water maze experiments were implemented,including 4 days' place swimming and 1 day's space exploration. Results After 1 month, diabetic rats' spatial memory ability and spatial associative memory ability were not affected. After 3 months, in place swimming test,the escape latency of two diabetic rat groups was obviously longer than that of the control group (P＜0.05). From the second day of the experiment, escape latency of the control descended sharply, while that of the diabetic group descended slowly. There was no difference between type 1 and type2 diabetic groups in escape latency (P＞0.05). After 3 months, in the space exploration test, when rats were put into the maze from I quadrant which already trained, swimming time in the platform quadrant was shorter, the other parameter scores were lower of the two diabetic model groups, contrast to the control group (P＜0.05). The parameter scores of type2 diabetic group were lower slightly than type1 diabetic group. When rats were put into the maze from IV quadrant for which never trained, the parameter scores of two diabetic groups were lower than that of the control group (P＜0.05) and the total score of type 1 group was lower than that of type2 group. Conclusion The spatial memory ability and spatial associative memory ability of type 1 and type2 diabetic rats descended. In the experiment, the spatial memory ability of type2 diabetic rats was more significantly affected than that of type1 diabetic rats. By contrast,type 1 diabetic rats' spatial associative memory ability descended greatly than that of type2 diabetic rats.
