CAJ | 학술논문

目的研究骨髓间充质干细胞(Bone Marrow Mesenchymal Stem Cells,BMSCs)及其分化生成的神经样细胞分别复合聚乳酸-羟基乙酸(Polylactic Glycolic Acid,PLGA)支架材料移植治疗大鼠脊髓损伤的效果.方法分别将单纯的PLGA、BMSCs-PLGA复合物以及神经样细胞-PLGA复合物移植入3组半横切的SD大鼠脊髓损伤模型中,对照组为直接缝合脊髓组.术后观察4组动物60d.结果 BMSCs及其分化生成的神经样细胞能在PLGA上良好的黏附、增殖和生长,两者复合移植后能促进脊髓及膀胱排尿功能的恢复.结论 BMSCs及神经样细胞复合PLGA移植可作为组织工程治疗脊髓损伤后膀胱功能的一种方法.
목적 연구골수간충질간세포(Bone Marrow Mesenchymal Stem Cells,BMSCs)급기분화생성적신경양세포분별복합취유산-간기을산(Polylactic Glycolic Acid,PLGA)지가재료이식치료대서척수손상적효과.방법 분별장단순적PLGA、BMSCs-PLGA복합물이급신경양세포-PLGA복합물이식입3조반횡절적SD대서척수손상모형중,대조조위직접봉합척수조.술후관찰4조동물60d.결과 BMSCs급기분화생성적신경양세포능재PLGA상량호적점부、증식화생장,량자복합이식후능촉진척수급방광배뇨공능적회복.결론 BMSCs급신경양세포복합PLGA이식가작위조직공정치료척수손상후방광공능적일충방법.
Objectives To study separately the effect of bone marrow mesenchymal stem cells(BMSCs)and differentiated neural-like cells combined with polylactic glycolic acid(PLGA)scaffolds,both composite which restore to SD rat,s bladder function after spinal cord injury.Methods Bold scaffold,BMSCs-PLGA complex,and neural-like cells-PLGA complex were separately transplanted into SD rat models with the transection for right spinal cord,the control group which is a direct suture half crosscut spinal cord group.And obsvered SD rat models during 60 days after operation.Results BMSCs and neural-like cells can be exactly adhere to,proliferate and grow on the PLGA;BMSCs and neural-like cells combined with PLGA,which can restore to spinal cord and promote the recovery of bladder function of SD rat models.Conclusions BMSCs and differentiated neural-like cells combined with PLGA scaffolds,which can be used as a method of tissue engineering,to treat bladder function caused by spinal cord injury.