中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2009年
6期
565-567
,共3页
陈洁%徐旋里%钟泰迪%王平
陳潔%徐鏇裏%鐘泰迪%王平
진길%서선리%종태적%왕평
虫草素%多糖类%呼吸窘迫综合征,成人%内毒素类
蟲草素%多糖類%呼吸窘迫綜閤徵,成人%內毒素類
충초소%다당류%호흡군박종합정,성인%내독소류
CORDYCEPIN%Polysaccharides%Respiratory distress syndrome,adult%Endotoxins
目的 评价虫草多糖预先给药对大鼠内毒素性急性肺损伤的影响.方法 雄性成年SD大鼠40只,体重190～220 g,随机分为5组(n=8):虫草多糖预先给药组(CP1-3,组)采用灌胃法分别给予虫草多糖1、2、3 g/ks,1次/12 h,连续6次,于最后1次给药后2 h时经股静脉注射内毒素5 mg/kg;急性肺损伤组(ALI组)以生理盐水1ml/100 g代替虫草多糖,其余方法同CP组;对照组(C组)以生理盐水1 ml/100 g代替虫草多糖和内毒素,其余方法同CP组.静脉注射内毒索后6 h时处死大鼠,计算肺湿干重比(W/D)、肺渗透指数(PPI);行支气管肺泡灌洗,对支气管肺泡灌洗液(BALF)行白细胞(WBC)和多形核白细胞(PMN)计数;测定肺组织及BALF肿瘤坏死因子α(TNF-α)水平,并行肺组织病理学评分.结果 与C组比较,ALI组和CP1～3组肺W/D、PPI、WBC和PMN计数、肺组织病理学评分、肺组织和BALF TNNF-α水平均升高(P<0.05或0.01);与ALI组比较,CP1组上述指标差异无统计学意义(P>0.05),CP2,3组上述指标降低(P<0.05);与CP2组比较,CP3组PMN计数、肺组织病理学评分、肺组织和BALFTNF-α水平降低(P<0.05).结论 虫草多糖预先给药可减轻大鼠内毒素性急性肺损伤,且呈剂量依赖性,其机制可能与虫草多糖降低肺组织炎性反应有关.
目的 評價蟲草多糖預先給藥對大鼠內毒素性急性肺損傷的影響.方法 雄性成年SD大鼠40隻,體重190～220 g,隨機分為5組(n=8):蟲草多糖預先給藥組(CP1-3,組)採用灌胃法分彆給予蟲草多糖1、2、3 g/ks,1次/12 h,連續6次,于最後1次給藥後2 h時經股靜脈註射內毒素5 mg/kg;急性肺損傷組(ALI組)以生理鹽水1ml/100 g代替蟲草多糖,其餘方法同CP組;對照組(C組)以生理鹽水1 ml/100 g代替蟲草多糖和內毒素,其餘方法同CP組.靜脈註射內毒索後6 h時處死大鼠,計算肺濕榦重比(W/D)、肺滲透指數(PPI);行支氣管肺泡灌洗,對支氣管肺泡灌洗液(BALF)行白細胞(WBC)和多形覈白細胞(PMN)計數;測定肺組織及BALF腫瘤壞死因子α(TNF-α)水平,併行肺組織病理學評分.結果 與C組比較,ALI組和CP1～3組肺W/D、PPI、WBC和PMN計數、肺組織病理學評分、肺組織和BALF TNNF-α水平均升高(P<0.05或0.01);與ALI組比較,CP1組上述指標差異無統計學意義(P>0.05),CP2,3組上述指標降低(P<0.05);與CP2組比較,CP3組PMN計數、肺組織病理學評分、肺組織和BALFTNF-α水平降低(P<0.05).結論 蟲草多糖預先給藥可減輕大鼠內毒素性急性肺損傷,且呈劑量依賴性,其機製可能與蟲草多糖降低肺組織炎性反應有關.
목적 평개충초다당예선급약대대서내독소성급성폐손상적영향.방법 웅성성년SD대서40지,체중190～220 g,수궤분위5조(n=8):충초다당예선급약조(CP1-3,조)채용관위법분별급여충초다당1、2、3 g/ks,1차/12 h,련속6차,우최후1차급약후2 h시경고정맥주사내독소5 mg/kg;급성폐손상조(ALI조)이생리염수1ml/100 g대체충초다당,기여방법동CP조;대조조(C조)이생리염수1 ml/100 g대체충초다당화내독소,기여방법동CP조.정맥주사내독색후6 h시처사대서,계산폐습간중비(W/D)、폐삼투지수(PPI);행지기관폐포관세,대지기관폐포관세액(BALF)행백세포(WBC)화다형핵백세포(PMN)계수;측정폐조직급BALF종류배사인자α(TNF-α)수평,병행폐조직병이학평분.결과 여C조비교,ALI조화CP1～3조폐W/D、PPI、WBC화PMN계수、폐조직병이학평분、폐조직화BALF TNNF-α수평균승고(P<0.05혹0.01);여ALI조비교,CP1조상술지표차이무통계학의의(P>0.05),CP2,3조상술지표강저(P<0.05);여CP2조비교,CP3조PMN계수、폐조직병이학평분、폐조직화BALFTNF-α수평강저(P<0.05).결론 충초다당예선급약가감경대서내독소성급성폐손상,차정제량의뢰성,기궤제가능여충초다당강저폐조직염성반응유관.
Objective To evaluate the effects of Cordyceps polysaccharide (CP) pretreatment on endotoxin-induced acute lung injury (ALI) in rats.Methods Forty adult male SD rats were randomly divided into 5 groups ( n = 8 each) : CP1-3 group, ALI group and control group (group C). Group CP1-3 received CP 1, 2 and 3 g/kg by intragastric gavage every 12 h for 6 times respectively, and then received LPS 5 mg/kg iv through the femoral vein 2 h after the last administration. Croup ALI received normal saline 1 ml/100 g instead of CP, and the rest methods were the same as those described in group CP. Group C received normal saline 1 ml/100 g instead of CP and LPS, and the rest methods were the same as those described in group CP. The animals were killed 6 h after injection of LPS. The lungs were removed for determination of W/D lung weight ratio, pulmonary permeability index (PPI), WBC and PMN counts in broncho-alveolar lavage fluid (BALF) , and levels of TNF-α in lung tissues and BALF, and microscopic examination.Results Compared with group C, W/D lung weight ratio, PPI, WBC and PMN counts in BALF, lung pathological scores and levels of TNF-α in lung tissues and BALF were significantly increased in group ALI and CP1-3 ( P < 0.05 or 0.01). No significant differences were found in the indices mentioned above between group CP1 and group ALI ( P > 0.05) . The indices mentioned above were significantly lower in group CP2,3 than in group ALI ( P < 0.05) . PMN counts in BALF, lung pathological scores and levels of TNF-α in lung tissues and BALF were significantly lower in group CP3 than in group CP2 ( P < 0.05) . Conclusion Cordyceps polysaccharide pretreatment can attenuate LPS-induced ALI in a dose-dependent manner through inhibition of inflammatory response in rats.
