中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2011年
3期
211-213
,共3页
实验性自身免疫性脑脊髓炎%氟西汀%脑源性神经营养因子%大鼠
實驗性自身免疫性腦脊髓炎%氟西汀%腦源性神經營養因子%大鼠
실험성자신면역성뇌척수염%불서정%뇌원성신경영양인자%대서
Experimental autoimmune encephalomylitis%Fluoxetine%Brain-derived neurotrophic factor%Rats
目的 初步探讨盐酸氟西汀对实验性自身免疫性脑脊髓炎(EAE)大鼠的干预作用和发病过程中不同时间点血清脑源性神经营养因子(BDNF)的表达.方法 将Wistar大鼠随机分为3组:溶媒对照组(n=6)、模型对照组(n=10)和氟西汀组(10mg/kg,n=10);采用自制豚鼠脊髓匀浆足垫注射免疫动物制作EAE模型,每天观察各组大鼠神经功能缺损评分;采用双抗夹心酶联免疫吸附试验(ELISA)分别检测大鼠免疫前、免疫后第16天及免疫后第25天血清BDNF的表达.结果 1、模型对照组及氟西汀组大鼠在免疫后第4天开始出现毛发无光泽及脱毛,在免疫后第8天开始出现鼠尾下垂,并逐渐发展至不同程度的肢体无力,在免疫后第16天动物发病达到高峰;溶媒对照组大鼠无发病.2、氟西汀显著促进了大鼠神经功能恢复,在免疫后第18天开始氟西汀组[(3.27±0.33)分]与模型对照组[(4.66±0.55)分]的临床神经功能缺损评分开始出现显著性差异(P＜0.05),并持续至实验结束.3、氟西汀组与模型对照组大鼠相比血清中BDNF的表达差异无显著性[氟西汀组(62.27±0.43)ng/L;模型对照组(61.67±0.85)ng/L,P＞0.05].结论 氟西汀显著促进了EAE大鼠的神经功能恢复,提示其具有治疗EAE动物的作用;氟西汀并不能通过促进EAE大鼠血清中BDNF的表达参与保护作用.
目的 初步探討鹽痠氟西汀對實驗性自身免疫性腦脊髓炎(EAE)大鼠的榦預作用和髮病過程中不同時間點血清腦源性神經營養因子(BDNF)的錶達.方法 將Wistar大鼠隨機分為3組:溶媒對照組(n=6)、模型對照組(n=10)和氟西汀組(10mg/kg,n=10);採用自製豚鼠脊髓勻漿足墊註射免疫動物製作EAE模型,每天觀察各組大鼠神經功能缺損評分;採用雙抗夾心酶聯免疫吸附試驗(ELISA)分彆檢測大鼠免疫前、免疫後第16天及免疫後第25天血清BDNF的錶達.結果 1、模型對照組及氟西汀組大鼠在免疫後第4天開始齣現毛髮無光澤及脫毛,在免疫後第8天開始齣現鼠尾下垂,併逐漸髮展至不同程度的肢體無力,在免疫後第16天動物髮病達到高峰;溶媒對照組大鼠無髮病.2、氟西汀顯著促進瞭大鼠神經功能恢複,在免疫後第18天開始氟西汀組[(3.27±0.33)分]與模型對照組[(4.66±0.55)分]的臨床神經功能缺損評分開始齣現顯著性差異(P＜0.05),併持續至實驗結束.3、氟西汀組與模型對照組大鼠相比血清中BDNF的錶達差異無顯著性[氟西汀組(62.27±0.43)ng/L;模型對照組(61.67±0.85)ng/L,P＞0.05].結論 氟西汀顯著促進瞭EAE大鼠的神經功能恢複,提示其具有治療EAE動物的作用;氟西汀併不能通過促進EAE大鼠血清中BDNF的錶達參與保護作用.
목적 초보탐토염산불서정대실험성자신면역성뇌척수염(EAE)대서적간예작용화발병과정중불동시간점혈청뇌원성신경영양인자(BDNF)적표체.방법 장Wistar대서수궤분위3조:용매대조조(n=6)、모형대조조(n=10)화불서정조(10mg/kg,n=10);채용자제돈서척수균장족점주사면역동물제작EAE모형,매천관찰각조대서신경공능결손평분;채용쌍항협심매련면역흡부시험(ELISA)분별검측대서면역전、면역후제16천급면역후제25천혈청BDNF적표체.결과 1、모형대조조급불서정조대서재면역후제4천개시출현모발무광택급탈모,재면역후제8천개시출현서미하수,병축점발전지불동정도적지체무력,재면역후제16천동물발병체도고봉;용매대조조대서무발병.2、불서정현저촉진료대서신경공능회복,재면역후제18천개시불서정조[(3.27±0.33)분]여모형대조조[(4.66±0.55)분]적림상신경공능결손평분개시출현현저성차이(P＜0.05),병지속지실험결속.3、불서정조여모형대조조대서상비혈청중BDNF적표체차이무현저성[불서정조(62.27±0.43)ng/L;모형대조조(61.67±0.85)ng/L,P＞0.05].결론 불서정현저촉진료EAE대서적신경공능회복,제시기구유치료EAE동물적작용;불서정병불능통과촉진EAE대서혈청중BDNF적표체삼여보호작용.
Objective To investigate whether fluoxetine has therapeutic effect of clinical score and brain derived neurotrophic factor (BDNF) expression in serum of experimental autoimmune encephalomyelitis (EAE)model. Methods Rats were randomly divided into solvent control group (n=6) ,model control group ( n= 10)and fluoxetine group ( n= 10). The EAE model was prepared by injecting guinea pig spinal cord homogenate subcutaneously. The clinical score was daily measured according to the sign and symptoms of rats in the behavior examination. The serum BDNF level was measured by EL1SA. Results 1. Except for the solvent control group,the first sign of EAE(Piloerection) was detected on 4th day after immunization of rats from both model control group and fluoxetine group,then EAE rats had distal tail weakness on 8th day, and gradually developed into completely tail paralysis and limb paralysis. EAE rats' clinical score reached the peak on 16th day after immunization. 2. The clinical score of fluoxetine group became scientifically lower than model group since 18th day after immunization ( Fluoxetine group :3.27 ± 0. 33; Model control group :4.66 ± 0. 55, P ＜ 0. 05 ). 3. Compared with the model control group,fluoxetine did not significantly increase the expression of serum BDNF in EAE model ( Fluoxetine group:62.27 ± 0.43; Model control group :61.67 ± 0.85, P ＞ 0.05 ). Conclusion Fluoxetine reduced the clinical score of EAE since 18th day after immunization,which indicates fluoxetine could promote the recovery of neurological function in EAE rats. BDNF may not contribute to protective effect of fluoxetine in EAE animal.
