中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2011年
7期
533-536
,共4页
姚晶晶%王征%陈岚%何淑蓉%于会艳%方芳%杨丽%乔旭柏%张伟%杜俊%秦斌%刘冬戈
姚晶晶%王徵%陳嵐%何淑蓉%于會豔%方芳%楊麗%喬旭柏%張偉%杜俊%秦斌%劉鼕戈
요정정%왕정%진람%하숙용%우회염%방방%양려%교욱백%장위%두준%진빈%류동과
阿尔茨海默病%白细胞介素1%基因型%多态性,限制性片段长度
阿爾茨海默病%白細胞介素1%基因型%多態性,限製性片段長度
아이자해묵병%백세포개소1%기인형%다태성,한제성편단장도
Alzheimer disease%Interleukin-1%Genotype%Polymorphism,restriction fragment length
目的 探讨白细胞介素1(interleukin-1,IL-1)基因型与阿尔茨海默病 (Alzheimer′s disease,AD)的关系.方法 应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,对84例AD和139例对照组标本进行IL-1A(-889)和 IL-1B (+3953)基因型分析.结果 AD组中IL-1A(-889)C/C、C/T和T/T基因型的频率分别为72.6%、23.8%和3.6%,对照组中C/C、C/T和T/T基因型的频率分别为84.2%、14.4%和1.4%,AD组和对照组IL-1A(-889)各基因型的频率差异无统计学意义(χ2=4.53,P＞0.05),但AD组IL-1A(-889)T等位基因的分布频率明显高于对照组,频率分别为15.5%(AD组)和8.6%(对照组)(χ2=4.93,P＜0.05).AD组中IL-1B (+3953) C/T基因型和T等位基因的频率分别为16.7%和8.3%,均显著高于对照组6.5%和3.2%(χ2=5.88、5.56,均P＜0.05).结论 IL-1基因型与AD的发生有关,可能是AD的重要危险因素.
目的 探討白細胞介素1(interleukin-1,IL-1)基因型與阿爾茨海默病 (Alzheimer′s disease,AD)的關繫.方法 應用聚閤酶鏈反應-限製性片段長度多態性(PCR-RFLP)技術,對84例AD和139例對照組標本進行IL-1A(-889)和 IL-1B (+3953)基因型分析.結果 AD組中IL-1A(-889)C/C、C/T和T/T基因型的頻率分彆為72.6%、23.8%和3.6%,對照組中C/C、C/T和T/T基因型的頻率分彆為84.2%、14.4%和1.4%,AD組和對照組IL-1A(-889)各基因型的頻率差異無統計學意義(χ2=4.53,P＞0.05),但AD組IL-1A(-889)T等位基因的分佈頻率明顯高于對照組,頻率分彆為15.5%(AD組)和8.6%(對照組)(χ2=4.93,P＜0.05).AD組中IL-1B (+3953) C/T基因型和T等位基因的頻率分彆為16.7%和8.3%,均顯著高于對照組6.5%和3.2%(χ2=5.88、5.56,均P＜0.05).結論 IL-1基因型與AD的髮生有關,可能是AD的重要危險因素.
목적 탐토백세포개소1(interleukin-1,IL-1)기인형여아이자해묵병 (Alzheimer′s disease,AD)적관계.방법 응용취합매련반응-한제성편단장도다태성(PCR-RFLP)기술,대84례AD화139례대조조표본진행IL-1A(-889)화 IL-1B (+3953)기인형분석.결과 AD조중IL-1A(-889)C/C、C/T화T/T기인형적빈솔분별위72.6%、23.8%화3.6%,대조조중C/C、C/T화T/T기인형적빈솔분별위84.2%、14.4%화1.4%,AD조화대조조IL-1A(-889)각기인형적빈솔차이무통계학의의(χ2=4.53,P＞0.05),단AD조IL-1A(-889)T등위기인적분포빈솔명현고우대조조,빈솔분별위15.5%(AD조)화8.6%(대조조)(χ2=4.93,P＜0.05).AD조중IL-1B (+3953) C/T기인형화T등위기인적빈솔분별위16.7%화8.3%,균현저고우대조조6.5%화3.2%(χ2=5.88、5.56,균P＜0.05).결론 IL-1기인형여AD적발생유관,가능시AD적중요위험인소.
Objective To explore the association of interleukin (IL)-1 genotypes with Alzheimer′s disease (AD). Methods Using polymerase chain reaction and restriction fragment length polymorphism, the IL-1A (-889) and IL-1B (+3953) genotypes in 84 cases of AD and 139 controls were detected and analyzed. Results The frequencies of IL-1A(- 889) C/C, C/T and T/T genotypes were 72.6% and 84.2%, 23.8% and 14.4%, 3.6% and 1.4% in AD cases and controls respectively. The genotypes frequencies of IL-1A (-889) C/C, C/T and T/T in AD cases were similar to that of controls (χ2=4.53, P＞0.05), but the frequencies of IL-1A (-889) T allele were significantly higher in AD cases than in controls (15.5% vs. 8.6%, χ2=4.93, P＜0.05). The frequencies of IL-1B (+3953) C/T genotypes and T allele were also significantly higher in AD cases than in controls (16.7% vs. 6.5%, 8.3% vs. 3.2%, χ2=5.88,5.56, both P＜0.05). Conclusions IL-1 genotypes are associated with AD. IL-1 genotypes may play an important role in the development of AD.
