CAJ | 학술논문

目的探讨肿瘤浸润淋巴细胞(TIL) CD3和人类白细胞抗原DR基因(HLA-DR)在大肠癌组织中的表达与患者生存预后的关系.方法应用免疫组织化学及流式细胞术测定60例大肠癌组织中CD3与HLA-DR表达,分析检测结果与临床病理特点及患者预后的关系.结果免疫组织化学检查显示肿瘤浸润淋巴细胞CD3和HLA-DR在60例大肠癌组织中阳性细胞表达率分别为23/60(38.33％)及25/60(41.67％),其表达与患者性别、年龄及淋巴结转移无明显相关,与大肠癌组织病理分化程度、肿瘤Dukes分期及患者预后相关.CD3和HLA-DR表达率与肿瘤的分化程度呈正相关,同时随大肠癌Dukes分期显示,Dukes分期越高其CD3和HLA-DR表达率越低；而≥5年生存率患者组CD3和HLA-DR表达率明显高于＜3年死亡患者组,差异有统计学意义(P＜0.05).经流式细胞术测定CD3、HLA-DR均呈低表达；CD3、HLA-DR在≥5年生存患者组表达率显著高于＜3年死亡组患者,差异有统计学意义(P＜ 0.05),与患者年龄、性别、淋巴结转移及组织病理分化程度无关.结论肿瘤浸润淋巴细胞CD3和HLA-DR可以作为判断大肠癌患者预后的重要参考指标之一.
목적 탐토종류침윤림파세포(TIL) CD3화인류백세포항원DR기인(HLA-DR)재대장암조직중적표체여환자생존예후적관계.방법 응용면역조직화학급류식세포술측정60례대장암조직중CD3여HLA-DR표체,분석검측결과여림상병리특점급환자예후적관계.결과 면역조직화학검사현시종류침윤림파세포CD3화HLA-DR재60례대장암조직중양성세포표체솔분별위23/60(38.33％)급25/60(41.67％),기표체여환자성별、년령급림파결전이무명현상관,여대장암조직병리분화정도、종류Dukes분기급환자예후상관.CD3화HLA-DR표체솔여종류적분화정도정정상관,동시수대장암Dukes분기현시,Dukes분기월고기CD3화HLA-DR표체솔월저；이≥5년생존솔환자조CD3화HLA-DR표체솔명현고우＜3년사망환자조,차이유통계학의의(P＜0.05).경류식세포술측정CD3、HLA-DR균정저표체；CD3、HLA-DR재≥5년생존환자조표체솔현저고우＜3년사망조환자,차이유통계학의의(P＜ 0.05),여환자년령、성별、림파결전이급조직병리분화정도무관.결론 종류침윤림파세포CD3화HLA-DR가이작위판단대장암환자예후적중요삼고지표지일.
Objective To explore the expression of tumor-infiltrating lymphocyte CD3 and human leukocyte antigen-DR (HLA-DR) in colorectal carcinoma and their prognostic significance.Methods Immunohistochemical technique (IHCA) and flow cytometry (FCM) were used to detect the expression of tumor-infiltrating lymphocyte CD3 and HLA-DR in 60 cases of colorectal carcinoma.The relationship of their expression with clinico-pathological features and prognosis of colorectal carcinoma was analyzed.Results The expression rate of CD3 and HLA-DR was respectively 38.33％ (23/60) and 41.67％ (25/60).The expression rate of CD3 and HLA-DR was correlated with tumor Dukes stage,differentiation degree of tumor and prognosis of patients,but not with patients' gender,age and lymph node metastasis.The expression of CD3 and HLA-DR was significantly increased in well-differentiated tumor group as compared with low-differentiated tumor group,and decreased with increases in Dukes staging.The expression levels of CD3 and HLA-DR were significantly higher in ≥ 5-year survival group than ＜ 3-year denth group ( P ＜0.05).Flow cytometry revealed the expression of CD3 and HLA-DR was significantly higher in ≥ 5-year survival group than in ＜ 3-year denth group ( P ＜ 0.05 ).The expression of CD3 and HLA-DR had no correlation with patients' gender,age,lymph node metastasis and differentiation degree of tumor.Conclusion TIL CD3 and HLA-DR are the important characteristic markers in colorectal carcinoma,and can be used to prodict the prognosis.