中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2011年
5期
363-366
,共4页
盂辉%李文才%王留兴%李文斌%张岚%樊青霞%王瑞林%路太英
盂輝%李文纔%王留興%李文斌%張嵐%樊青霞%王瑞林%路太英
우휘%리문재%왕류흥%리문빈%장람%번청하%왕서림%로태영
乳腺肿瘤%拓扑异构酶Ⅱα%荧光原位杂交%实时定量PCR%基因扩增%预后
乳腺腫瘤%拓撲異構酶Ⅱα%熒光原位雜交%實時定量PCR%基因擴增%預後
유선종류%탁복이구매Ⅱα%형광원위잡교%실시정량PCR%기인확증%예후
Breast neoplasms%Topoisomerase Ⅱ Alpha%Fluorescence in situ hybridization%Quantitative RT-PCR%Gene amplification%Prognosis
目的 探讨拓扑异构酶Ⅱα(TOP2A)的表达与乳腺癌患者生存的相关性.方法 通过实时定量逆转录聚合酶链反应(quantitative RT-PCR)方法检测86例乳腺癌组织中的TOP2A基因表达量,应用荧光原位杂交(FISH)检测乳腺癌组织中TOP2A基因扩增,应用免疫组化(IHC)法检测TOP2A蛋白表达,并分析TOP2A基因表达量、TOP2A基因扩增与TOP2A蛋白表达的相关性.结果 86例乳腺癌患者中,TOP2A基因扩增11例,TOP2A基因缺失11例,TOP2A基因变异率为25.6%(22/86);TOP2A蛋白表达的阳性率为66.3%(57/86).TOP2A基因表达水平与蛋白表达水平显著相关(P=0.001),TOP2A基因表达水平与乳腺癌患者的无进展生存期显著相关(P<0.001).TOP2A基因扩增与TOP2A蛋白阳件表达无显著相关性(P=0.211).结论 TOP2A的基因定量表达水平为乳腺癌客观可靠的临床预后指标.
目的 探討拓撲異構酶Ⅱα(TOP2A)的錶達與乳腺癌患者生存的相關性.方法 通過實時定量逆轉錄聚閤酶鏈反應(quantitative RT-PCR)方法檢測86例乳腺癌組織中的TOP2A基因錶達量,應用熒光原位雜交(FISH)檢測乳腺癌組織中TOP2A基因擴增,應用免疫組化(IHC)法檢測TOP2A蛋白錶達,併分析TOP2A基因錶達量、TOP2A基因擴增與TOP2A蛋白錶達的相關性.結果 86例乳腺癌患者中,TOP2A基因擴增11例,TOP2A基因缺失11例,TOP2A基因變異率為25.6%(22/86);TOP2A蛋白錶達的暘性率為66.3%(57/86).TOP2A基因錶達水平與蛋白錶達水平顯著相關(P=0.001),TOP2A基因錶達水平與乳腺癌患者的無進展生存期顯著相關(P<0.001).TOP2A基因擴增與TOP2A蛋白暘件錶達無顯著相關性(P=0.211).結論 TOP2A的基因定量錶達水平為乳腺癌客觀可靠的臨床預後指標.
목적 탐토탁복이구매Ⅱα(TOP2A)적표체여유선암환자생존적상관성.방법 통과실시정량역전록취합매련반응(quantitative RT-PCR)방법검측86례유선암조직중적TOP2A기인표체량,응용형광원위잡교(FISH)검측유선암조직중TOP2A기인확증,응용면역조화(IHC)법검측TOP2A단백표체,병분석TOP2A기인표체량、TOP2A기인확증여TOP2A단백표체적상관성.결과 86례유선암환자중,TOP2A기인확증11례,TOP2A기인결실11례,TOP2A기인변이솔위25.6%(22/86);TOP2A단백표체적양성솔위66.3%(57/86).TOP2A기인표체수평여단백표체수평현저상관(P=0.001),TOP2A기인표체수평여유선암환자적무진전생존기현저상관(P<0.001).TOP2A기인확증여TOP2A단백양건표체무현저상관성(P=0.211).결론 TOP2A적기인정량표체수평위유선암객관가고적림상예후지표.
Objective The aim of this study was to assess the TOP2A RNA expression and die relationship of TOP2A protein expression with metastasis-free interval in breast cancer patients. Methods TOP2A expression was analyzed prior to surgery in 86 patients. The level of TOP2A gene amplification was analyzed by fluorescence in situ hybridization (FISH) , its RNA expression level with RT-PCR, and their correlation with TOP2A protein expression was assessed by immunohistochemistry (IHC). The correlation between RNA expression level and metastasis-free interval in breast cancer patients was also analyzed. Results Aberrations (amplification or deletion) of TOP2A copy number was observed in 25.6% (22/86) of the cases. TOP2A protein expression was detected in 66. 3% (57/86) of the samples. There was a significant correlation between the TOP2A RNA expression and protein expression (P<0.001). TOP2A gene expression was significantly associated with the metastasis-free interval in the breast cancer patients (P =0.001). There was no significant correlation between TOP2A gene amplification and TOP2A protein expression (P=0.211). Conclusions TOP2A RNA level is an objective and reliable prognostic indicator in breast cancer.
