CAJ | 학술논문

过氧化物酶在植物的损伤愈合中起着重要作用.我们采用生物化学分析和组织定位技术,确定了杜仲(Eucommia ulmoides Oliv.)剥皮再生过程中形成层区域过氧化物酶的分布和活性变化.剥皮后第4天至第21天,剥皮植株过氧化物酶含量大幅度增加(与对照比,增加了30～40倍),在整个剥皮再生过程中(从第0天至第63天),过氧化物酶在细胞分裂活跃的形成层和木栓形成层细胞中分布较少.而在愈伤组织、类皮层、成熟韧皮部和成熟木质部中分布较多.过氧化物酶在形成层及其两侧呈梯度分布,形成层中最少,在两侧的韧皮部和木质部中依次递增.其等电聚焦电泳显示两条酶带:POD Ⅰ和PODⅡ.前者只在木质部中分布,可能与木质部的分化有关;后者只在再生的组织中分布,可能与树皮再生过程有关.
과양화물매재식물적손상유합중기착중요작용.아문채용생물화학분석화조직정위기술,학정료두중(Eucommia ulmoides Oliv.)박피재생과정중형성층구역과양화물매적분포화활성변화.박피후제4천지제21천,박피식주과양화물매함량대폭도증가(여대조비,증가료30～40배),재정개박피재생과정중(종제0천지제63천),과양화물매재세포분렬활약적형성층화목전형성층세포중분포교소.이재유상조직、류피층、성숙인피부화성숙목질부중분포교다.과양화물매재형성층급기량측정제도분포,형성층중최소,재량측적인피부화목질부중의차체증.기등전취초전영현시량조매대:POD Ⅰ화PODⅡ.전자지재목질부중분포,가능여목질부적분화유관;후자지재재생적조직중분포,가능여수피재생과정유관.
Peroxidases are known to play important roles in plant wound healing. Biochemical analysis and histochemical localization techniques were used to assess changes and distribution of peroxidases in the recovering bark after girdling in Eucommia ulmoides Oliy. Between 4 and 21 days after girdling (DAG),peroxidases activity in the girdled trees significantly increased by 30-40 times over that in ungirdled trees.During the whole bark recovery process (from 0 to 63 DAG), the peroxidase signal was not found in the tissue regions subjected to intense cell division activity (regenerating cambial zone and phellogen). However,high peroxidase activity was detected in the callus, cortex-like, mature phloem and xylem. Interestingly, it was shown that, in maturing xylem and phloem cells, there was respectively an inward and outward peroxidase activity gradient on both sides of the cambium zone. An isoelectric-focusing electrophoresis of the extracted protein displayed two isozyme bands of peroxidase: POD Ⅰ and POD Ⅱ. POD Ⅰ was only detected in the xylem fraction and could play a role in xylem differentiation. POD Ⅱ was only identified in the recovering bark portion and could be more engaged in bark regeneration process. A relationship between IAA and peroxidase is also discussed.