CAJ | 학술논문

运用国际法医学界推荐的"最小单倍型"共7个微卫星位点(DYS19,DYS389Ⅰ,DYS389Ⅱ,DYS390,DYS391,DYS392,DYS393)和1个高多态性微卫星位点DYS385,采用PCR结合银染显色技术,对采自临夏回族自治区东乡县的133个健康男性个体的DNA,进行了Y染色体8个微卫星的等位基因及单倍型分布状况遗传多态性分析.结果显示,东乡族保留着较高的Y-STR遗传多态性,在测出的66个等位基因中共构建了110种单倍型,单倍型多样性为98.9%,表明该系统有较强的个体识别能力.另外,我们的研究可以为东乡族这一穆斯林民族的起源提供有价值的遗传资料.
운용국제법의학계추천적"최소단배형"공7개미위성위점(DYS19,DYS389Ⅰ,DYS389Ⅱ,DYS390,DYS391,DYS392,DYS393)화1개고다태성미위성위점DYS385,채용PCR결합은염현색기술,대채자림하회족자치구동향현적133개건강남성개체적DNA,진행료Y염색체8개미위성적등위기인급단배형분포상황유전다태성분석.결과현시,동향족보류착교고적Y-STR유전다태성,재측출적66개등위기인중공구건료110충단배형,단배형다양성위98.9%,표명해계통유교강적개체식별능력.령외,아문적연구가이위동향족저일목사림민족적기원제공유개치적유전자료.
Eight Y-chromosomal microsatellites have been used to analyze DNA variation in 133 Dongxiang male subjects from the Muslim minority in Northwest Chinese, a set of seven Y-chromosomal STR loci (DYS19, DYS389 Ⅰ, DYS389 Ⅱ, DYS390, DYS391, DYS392, DYS393) with the addition of the biloci marker DYS385 was used to generate male-specific haplotype databases for the Chinese ethnic group samples and PCR products were separated by manual procedures. Allele distribution frequencies and gene diversity value at seven Y-Chromosome STR loci in the Dongxiang population were calculated. The distribution of Y-STR haplotypes is presented. A total of 110 different haplotypes were found, 94 of them being unique. The Y-STR polymorphism in the Northwest Chinese population with an estimated haplotype diversity of 98.9% and a discrimination capacity of 82.7% will be very useful for testing paternal lineages. This work has an important implication on forensic and anthropological studies.